TCHP (NM_001143852) Human Tagged ORF Clone
CAT#: RC227291
TCHP (Myc-DDK-tagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2
"NM_001143852" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TCHP |
Synonyms | TpMs |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227291 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGACAGC GAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGGATGTCTGACATCTG CAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGGAGCATGCATGCCTATCAGCGGGAG AAGATGAAGGAGGAGAAGAGGAGGAGTCTGGAGGCCCGACGGGAAAAGCTCAGGCAGCTCATGCAGGAGG AGCAGGACCTGCTGGCCAGAGAACTGGAGGAGCTGAGGCTGAGCATGAACTTGCAGGAAAGAAGAATCCG GGAGCAGCACGGGAAGCTGAAATCAGCCAAAGAAGAGCAGAGGAAACTGATTGCTGAACAACTTTTGTAC GAACACTGGAAAAAGAACAACCCGAAACTTCGAGAGATGGAGCTGGACCTTCACCAGAAGCATGTCGTAA ACTCTTGGGAAATGCAGAAAGAAGAAAAAAAACAGCAAGAAGCCACCGCAGAGCAAGAGAACAAACGGTA TGAAAATGAATATGAAAGGGCCCGAAGGGAGGCGCTAGAAAGGATGAAAGCTGAAGAGGAGAGGAGGCAG CTGGAGGACAAGCTCCAGGCCGAGGCACTGCTGCAACAGATGGAGGAGCTGAAGCTGAAGGAGGTGGAGG CGACCAAACTAAAGAAGGAGCAGGAGAATCTGTTGAAGCAGCGGTGGGAGCTAGAGAGGCTGGAGGAAGA GCGAAAGCAGATGGAAGCCTTCCGGCAGAAGGCAGAGCTGGGGCGTTTCTTGAGACATCAGTATAACGCT CAACTCAGCAGACGCACACAGCAGATCCAAGAGGAGCTGGAGGCAGACAGGCGGATCCTGCAGGCCCTCC TCGAGAAGGAGGACGAGAGCCAGCGCCTCCACCTGGCCAGGCGGGAGCAGGTCATGGCCGATGTGGCCTG GATGAAGCAGGCCATTGAGGAGCAGCTGCAGCTGGAGCGGGCGCGGGAGGCAGAGCTGCAGATGCTGCTG AGGGAGGAGGCCAAGGAGATGTGGGAAAAGAGAGAGGCAGAGTGGGCCCGAGAGCGCAGCGCACGGGACA GACTGATGAGCGAGGTTCTGACAGGGAGACAACAGCAAATACAAGAGAAGATTGAGCAGAACCGACGGGC ACAAGAGGAATCCCTGAAACACAGGGAGCAACTTATTCGAAATCTTGAGGAGGTGAGAGAGTTGGCTCGT CGCGAGAAAGAGGAGAGTGAAAAGCTGAAATCGGCCAGGAAGCAGGAGCTGGAAGCCCAGGTTGCAGAGC GCCGGCTGCAGGCATGGGAAGCAGACCAGCAGGAGGAGGAGGAAGAGGAGGAGGCCCGGCGGGTCGAGCA GCTCTCAGATGCCCTGCTGCAGCAGGAGGCGGAGACTATGGCTGAGCAGGGCTACCGGCCTAAGCCTTAC GGACATCCAAAAATTGCTTGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227291 protein sequence
Red=Cloning site Green=Tags(s) MALPTLPSYWCSQQRLNQQLARQREQEARLRQQWEQNSRYFRMSDICSSKQAEWSSKTSYQRSMHAYQRE KMKEEKRRSLEARREKLRQLMQEEQDLLARELEELRLSMNLQERRIREQHGKLKSAKEEQRKLIAEQLLY EHWKKNNPKLREMELDLHQKHVVNSWEMQKEEKKQQEATAEQENKRYENEYERARREALERMKAEEERRQ LEDKLQAEALLQQMEELKLKEVEATKLKKEQENLLKQRWELERLEEERKQMEAFRQKAELGRFLRHQYNA QLSRRTQQIQEELEADRRILQALLEKEDESQRLHLARREQVMADVAWMKQAIEEQLQLERAREAELQMLL REEAKEMWEKREAEWARERSARDRLMSEVLTGRQQQIQEKIEQNRRAQEESLKHREQLIRNLEEVRELAR REKEESEKLKSARKQELEAQVAERRLQAWEADQQEEEEEEEARRVEQLSDALLQQEAETMAEQGYRPKPY GHPKIAWN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001143852 |
ORF Size | 1494 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001143852.1, NP_001137324.1 |
RefSeq Size | 3111 bp |
RefSeq ORF | 1497 bp |
Locus ID | 84260 |
MW | 61.1 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325135 | TCHP (untagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
USD 660.00 |
|
RG227291 | TCHP (GFP-tagged) - Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
USD 460.00 |
|
RC227291L3 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC227291L4 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, mGFP tagged |
USD 620.00 |
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