POLR1B (NM_001137604) Human Tagged ORF Clone

CAT#: RC227307

POLR1B (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR1B
• Synonyms: RPA2; RPA135; Rpo1-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227307 representing NM_001137604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCTT
  ATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAA
  CTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCTGATATCAACTGGGCAGTGAATGGAATCTCA
  AAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATGGTGAAATCCAAGCTTTGCAACTTACGTA
  ACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAA
  TGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAGACCA
  AAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGGGAAGAACATT
  CCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTTATTTACCGAAAAGA
  ACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTCAGCTTTTCTGATTATCAGATCTTTCAG
  GAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAACTCTGTTTCTCAGATGTTAAGGATTGTAA
  TGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAA
  TGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTG
  AAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGGAG
  AGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTCTTCCTTAT
  GTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATTAAAATAGCTTTTGATAAGAAGGCTCAGAAG
  ACCAGTGTTTCCATGAACACTGACAATTTGATGAGGATTTTTACAATGGGCATAGACCTTACAAAACCAT
  TTGAATACCTTTTTGCTACTGGGAATCTGCGTTCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGACT
  TTGTGTTGTGGCTGACAAGCTGAACTTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCT
  GATTTTGCCAAGATGAGGACCACCACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCG
  TGCATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCCGTATGTGAGGTTGTCACACA
  GTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAACTTGGGGGTCACTCCCATTGATGGAGCTCCC
  CACCGATCATACAGTGAGTGCTACCCTGTCCTGCTGGACGGTGTCATGGTTGGCTGGGTGGATAAGGATC
  TTGCTCCAGGCATCGCAGATTCTCTTCGTCATTTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGAT
  GGAAGTGGTCCTTATACCCATGACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCT
  TGTAGACTGGTACGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGA
  TCTTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCC
  ACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATG
  TACCAATGCCAGATGGGTAAGCAAACTATGGGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACA
  AACTGTATCGTCTTCAGACTCCTCAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATGGA
  TAACTATCCAATTGGGACCAATGCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCC
  ATGATTGTGAATAAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAG
  ACCTCTCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGT
  TCTGCAGAAGTTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTAC
  AGCTACCTCAACCTCAACACCGGGGAAAGTTTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGG
  ATAACATCAAAGTGTGCAGTAATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGT
  GCCTCGGAACCCAACTATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTG
  TGGCCGGCTGAGGACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTT
  TTCCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCT
  CTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTA
  AAGGCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAG
  CAGACATCTTCATAGGAGTGGTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAG
  GACAACTGGAGCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGT
  TTTGGGGAGATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCA
  ACTGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAA
  GCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACACT
  ATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAG
  TGAAACTGGATGTTGTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227307 representing NM_001137604
  Red=Cloning site Green=Tags(s)
  
  MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQADINWAVNGIS
  KGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRP
  KWKTRGPGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQ
  ELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHL
  KSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQK
  TSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGA
  DFAKMRTTTVRRLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAP
  HRSYSECYPVLLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTP
  CRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNM
  YQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDA
  MIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYY
  SYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRL
  WPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEML
  KAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIR
  FGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDT
  IDTVSVPYVFRYFVAELAAMNIKVKLDVV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001137604
• ORF Size: 3237 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001137604.1, NM_001137604.2, NP_001131076.1
• RefSeq ORF: 3240
• Locus ID: 84172
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 121.8 kDa
• Gene Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]). [supplied by OMIM, Mar 2008]