RNF156 (MGRN1) (NM_001142290) Human Tagged ORF Clone

CAT#: RC227366

MGRN1 (Myc-DDK-tagged)-Human mahogunin, ring finger 1 (MGRN1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY428013) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227366 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGRN1
• Synonyms: RNF156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227366 representing NM_001142290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTATC
  GCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACCCC
  CCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAGTTT
  CCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAAGACT
  CCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGGGTGCT
  CTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCATCGGAG
  GAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTCCACTACA
  AGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAGGATGACGA
  GCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGAGATGTG
  GTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGCAGCTTCTCTG
  TGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATCTATGGCATTGA
  GAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAACGAGTGTGTGGTG
  TGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGTACCTCCTGCGCCG
  ACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCCCTCCTGCAGATCCG
  GGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTGGCCCAGAGCCTGGAG
  CATGATGAGCACTCTTGTCCCTTTAAAAAATCAAAGCCGCACCCCGCCTCCCTGGCCAGCAAGAAACCTA
  AAAGGGAAACAAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGCTCAACGG
  CCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGGCATCTCG
  GACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATCCTGGACAGCAGCCGCCAGAAGG
  GCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCTTCCCCCATCCACGAAGAGGATGA
  GGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGCGCAGAGCTGGCCCTGCGGGAAAGC
  AGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCGTCACCACAGCAAGGGACCCGAGCAG
  CTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGTGGCCGAGGCCCACCTGCTGACATCTA
  CCTGCCAGCCCTGGGGCCCGACTCCTGCTCTGTTGGTATAGACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227366 representing NM_001142290
  Red=Cloning site Green=Tags(s)
  
  MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF
  PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASE
  EFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDV
  VEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECVV
  CLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLE
  HDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGIS
  DGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRES
  SSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPALGPDSCSVGIDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142290
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142290.1, NM_001142290.2, NP_001135762.1
• RefSeq ORF: 1659
• Locus ID: 23295
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 60.6 kDa
• Gene Summary: Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro. [supplied by OMIM, Apr 2004]