LIPT2 (NM_001144869) Human Tagged ORF Clone

CAT#: RC227410

LIPT2 (Myc-DDK-tagged)-Human lipoyl(octanoyl) transferase 2 (putative) (LIPT2), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY428532) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227410 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227410 representing NM_001144869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCAACCCGCCGTTCGGTTGGTGCGCCTGGGTCGGGTGCCGTACGCCGAGCTACTGGGGCTGCAGG
  ACCGCTGGCTGCGGCGGCTGCAGGCCGAGCCAGGCATTGAGGCCCCGTCGGGGACTGAGGCGGGCGCGCT
  CCTGCTCTGCGAGCCCGCGGGGCCCGTGTATACGGCCGGGCTGCGCGGCGGCCTGACGCCCGAGGAAACT
  GCGCGGCTACGGGCCTTGGGCGCCGAGGTGCGCGTCACAGGCCGCGGTGGCCTGGCCACCTTCCACGGCC
  CGGGCCAGCTGCTTTGCCACCCGGTACTCGACCTGCGGCGTCTCGGCCTGCGCTTGCGCATGCACGTAGC
  GTCGCTGGAGGCGTGCGCCGTGCGCCTGTGCGAGCTCCAGGGCCTGCAGGACGCCCGCGCGCGGCCCCCG
  CCCTACACTGGCGTCTGGCTAGACGATCGCAAGATCTGCGCGATCGGAGTCCGCTGTGGAAGGCACATCA
  CATCCCACGGCCTGGCTCTCAACTGCTCTACCGACCTCACGTGGTTTGAGCACATCGTGCCCTGTGGACT
  GGTTGGGACAGGCGTCACTTCCTTGAGTAAGGAGCTCCAGAGGCACGTCACCGTGGAAGAAGTAATGCCA
  CCTTTCCTTGTGGCCTTTAAGGAGATCTACAAGTGCACACTGATCTCAGAGGACAGCCCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227410 representing NM_001144869
  Red=Cloning site Green=Tags(s)
  
  MRQPAVRLVRLGRVPYAELLGLQDRWLRRLQAEPGIEAPSGTEAGALLLCEPAGPVYTAGLRGGLTPEET
  ARLRALGAEVRVTGRGGLATFHGPGQLLCHPVLDLRRLGLRLRMHVASLEACAVRLCELQGLQDARARPP
  PYTGVWLDDRKICAIGVRCGRHITSHGLALNCSTDLTWFEHIVPCGLVGTGVTSLSKELQRHVTVEEVMP
  PFLVAFKEIYKCTLISEDSPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144869
• ORF Size: 693 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001144869.1, NM_001144869.2, NP_001138341.1
• RefSeq ORF: 696
• Locus ID: 387787
• Protein Pathways: Lipoic acid metabolism, Metabolic pathways
• MW: 25 kDa
• Gene Summary: This gene encodes a mitochondrial protein that catalyzes the transfer of octanoic acid to lipoate-dependent enzymes such as octanoyl-ACP. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]