SLC30A7 (NM_001144884) Human Tagged ORF Clone

CAT#: RC227524

SLC30A7 (Myc-DDK-tagged)-Human solute carrier family 30 (zinc transporter), member 7 (SLC30A7), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428544) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227524 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC30A7 protein (Cat# TP327524). The protein was produced from HEK293T cells transfected with SLC30A7 cDNA clone (Cat# RC227524) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC30A7
• Synonyms: ZnT-7; ZNT7; ZnTL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227524 representing NM_001144884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCCTGTCCATCAAAGACGATGAATACAAACCACCCAAGTTCAATTTGTTCGGCAAGATCTCGG
  GCTGGTTTAGGTCTATACTGTCCGACAAGACTTCCCGGAACCTGTTTTTCTTCCTGTGCCTGAACCTCTC
  TTTCGCTTTTGTGGAACTACTCTACGGCATCTGGAGCAACTGCTTAGGCTTGATTTCCGACTCTTTTCAC
  ATGTTTTTCGATAGCACTGCCATTTTGGCTGGACTGGCAGCTTCTGTTATTTCAAAATGGAGAGATAATG
  ATGCTTTCTCCTATGGGTATGTTAGAGCGGAAGTTCTGGCTGGCTTTGTCAATGGCCTATTTTTGATCTT
  CACTGCTTTTTTTATTTTCTCAGAAGGAGTTGAGAGAGCATTAGCCCCTCCAGATGTCCACCATGAGAGA
  CTGCTTCTTGTTTCCATTCTTGGGTTTGTGGTAAACCTAATAGGAATATTTGTTTTCAAACATGGAGGTC
  ATGGACATTCTCATGGCTCTGGCCACGGACACAGTCATTCCCTCTTTAATGGTGCTCTAGATCAGGCACA
  TGGCCATGTCGATCATTGCCATAGCCATGAAGTGAAACATGGTGCTGCACATAGCCATGATCATGCTCAT
  GGACATGGACACTTTCATTCTCATGATGGCCCGTCCTTAAAAGAAACAACAGGACCCAGCAGACAGATTT
  TACAAGGTGTATTTTTACATATCCTAGCAGATACACTTGGAAGTATTGGTGTAATTGCTTCTGCCATCAT
  GATGCAAAATTTTGGTCTGATGATAGCAGATCCTATCTGTTCAATTCTTATAGCCATTCTTATAGTTGTA
  AGTGTTATTCCTCTTTTAAGAGAATCTGTTGGAATATTAATGCAGAGAACTCCTCCCCTATTAGAAAATA
  GTCTGCCTCAGTGCTATCAGAGGGTACAGCAGTTGCAAGGAGTTTACAGTTTACAGGAACAGCACTTCTG
  GACTTTATGTTCTGACGTTTATGTTGGGACCTTGAAATTAATAGTAGCACCTGATGCTGATGCTAGGTGG
  ATTTTAAGCCAAACACATAATATTTTTACTCAGGCTGGAGTGAGACAGCTCTACGTACAGATTGACTTTG
  CAGCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227524 representing NM_001144884
  Red=Cloning site Green=Tags(s)
  
  MLPLSIKDDEYKPPKFNLFGKISGWFRSILSDKTSRNLFFFLCLNLSFAFVELLYGIWSNCLGLISDSFH
  MFFDSTAILAGLAASVISKWRDNDAFSYGYVRAEVLAGFVNGLFLIFTAFFIFSEGVERALAPPDVHHER
  LLLVSILGFVVNLIGIFVFKHGGHGHSHGSGHGHSHSLFNGALDQAHGHVDHCHSHEVKHGAAHSHDHAH
  GHGHFHSHDGPSLKETTGPSRQILQGVFLHILADTLGSIGVIASAIMMQNFGLMIADPICSILIAILIVV
  SVIPLLRESVGILMQRTPPLLENSLPQCYQRVQQLQGVYSLQEQHFWTLCSDVYVGTLKLIVAPDADARW
  ILSQTHNIFTQAGVRQLYVQIDFAAM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144884
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144884.1, NP_001138356.1
• RefSeq ORF: 1131 bp
• Locus ID: 148867
• Protein Families: Transmembrane
• MW: 41.4 kDa
• Gene Summary: Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc (Seve et al., 2004 [PubMed 15154973]). [supplied by OMIM, Mar 2008]