HBS1L (NM_001145207) Human Tagged ORF Clone

CAT#: RC227618

HBS1L (Myc-DDK-tagged)-Human HBS1-like (S. cerevisiae) (HBS1L), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HBS1L
• Synonyms: EF-1a; eRF3c; ERFS; HBS1; HSPC276
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227618 representing NM_001145207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCATCGGAATGTTCGAGGCTATAACTACGATGAAGATTTTGAAGATGATGATCTCTACGGCC
  AGTCTGTAGAGGATGATTATTGTATTTCGCCGTCAACAGCTGCTCAGTTTATTTATTCACGGCGTGACAA
  ACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGATCTGAAAGAATCTTCCAATTCTGTTTCAAAC
  CATCAGCTCAGTGGATTTGATCAAGCTCGTCTTTATTCATGCCTTGATCACATGAGAGAGGTACTTGGAG
  ATGCTGTGCCAGATGAAATATTAATTGAAGCAGTTCTGAAGAACAAGTTTGATGTGCAGAAGGCTTTGTC
  AGGGGTTCTGGAACAAGATAGAGTGCAGAGTTTGAAGGACAAGAATGAGGCAACAGTATCTACAGGAAAG
  ATAGCAAAAGGAGTCTTATTTTCTTCTTCTGAAGTTTCAGCTGATAATGTTCAAAGTTCTTATCCTCAGT
  CAGCAAATCATTTGGATTATAGTAGCAAACCCTTTGATTTTGCTAGCTCAGTAGGAAAATACGGATTATC
  TCATAATTCTTCAGTTCCAACTCACTGTTTACTCCATAGAAAAAAGAAACTTGACACACGTAAAAGTGAA
  AAGAAACTAGAATCATGTAAGTTAACAAAAGAACTTTCACTAGCTAACCTAATTCATGATATGTCAAGAG
  ATTCCTGTGAAAGTCAGCCATCAGTCAGATTATCATCTACAGACAGTCTGGAAAGTCTGCTTTCAAAGAA
  CCTAGATGCCGATTTGTTGAGACCTCATGCATCTGAATGCATTTCCAAAGATGATTCTGCATTCAAAGAA
  ATACCAGATTTAAAGACCATAATCATAAAGGGCACAACACCGAATAATTCTTTATATATTCAAAATAATT
  CACTGTCTGATTTTCAAAACATTCCAGTACAGGACAGTTTAGGAAGTTCAAATAATCCTTTGTACTTAAC
  TAGCTCGTTGGAAAATATGACTGTTGATAATTTAAATGCTAGTAAAGAAACTGAAGTTGGAAATGTTTCT
  TTAGTTGAACAAAGTGCCAAGAATCACACTTTTAAAAATGATAATTTGCAGTTTTCTCAGTGTGAAAGTC
  CATCCCTAACTGAACTGTTTCAGGAACACAAAGAAAACAATATAAGCCAGTGCTTTACTTTATCTGACCT
  TTGTAACCAGTCATCTGCCAGTTTCACAGATCTAAGTTTGGGATCCTTTCCCCTGTCACAATTAGCAAAT
  CGCTGTCAGTCTTCACCCGGAATATCAGAATTAACAGGATCTCTGTCGTCATTGGCATTTCATAAAGCTT
  CTCCCACAAGAGACCTTGAGAATTTGTCACTTTCTGAATTGATTGCAGAAACAATTGATGTAGACAACTC
  TCAGATTAAGAAAGAGTCCTTTGAGGTCAGTTTATCTGAAGTGAGGTCTCCTGGAATAGATTCAAATATT
  GATCTTAGTGTCCTTATAAAAAACCCAGATTTTGTTCCAAAGCCTGTAGTAGACCCATCCATTGCTCCAT
  CATCACGAACTAAAGTTCTAAGTTCAAAGCTAGGGAAGAATTCCAATTTTGCTAAGGATAATAAGAAAAA
  CAACAAAGGCTCTCTGACTAGGAAACCACCTTTTTCTCTCTCTTGGACCAAGGCCCTTGCTGCTAGACCT
  TCAGCTTTTGCCTCAACACTGTGTCTTCGTTACCCACTGAAAAGCTGCAAGCGACGCACCCTTGACCTCT
  ATAAGACTTTTCTTTATAGTAGACAAGTTCAAGATGTAAAGGACAAAGAAATAAGTCCACTTGTAGCAAT
  AACACCATTTGACTTCAAATCAGCATCACCTGATGACATTGTAAAAGCTAATCAAAAAAAAGCATTTACT
  AGAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227618 representing NM_001145207
  Red=Cloning site Green=Tags(s)
  
  MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSN
  HQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGK
  IAKGVLFSSSEVSADNVQSSYPQSANHLDYSSKPFDFASSVGKYGLSHNSSVPTHCLLHRKKKLDTRKSE
  KKLESCKLTKELSLANLIHDMSRDSCESQPSVRLSSTDSLESLLSKNLDADLLRPHASECISKDDSAFKE
  IPDLKTIIIKGTTPNNSLYIQNNSLSDFQNIPVQDSLGSSNNPLYLTSSLENMTVDNLNASKETEVGNVS
  LVEQSAKNHTFKNDNLQFSQCESPSLTELFQEHKENNISQCFTLSDLCNQSSASFTDLSLGSFPLSQLAN
  RCQSSPGISELTGSLSSLAFHKASPTRDLENLSLSELIAETIDVDNSQIKKESFEVSLSEVRSPGIDSNI
  DLSVLIKNPDFVPKPVVDPSIAPSSRTKVLSSKLGKNSNFAKDNKKNNKGSLTRKPPFSLSWTKALAARP
  SAFASTLCLRYPLKSCKRRTLDLYKTFLYSRQVQDVKDKEISPLVAITPFDFKSASPDDIVKANQKKAFT
  RE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145207
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145207.1, NP_001138679.1
• RefSeq ORF: 1899
• Locus ID: 10767
• MW: 69.9 kDa
• Gene Summary: This gene encodes a member of the GTP-binding elongation factor family. It is expressed in multiple tissues with the highest expression in heart and skeletal muscle. The intergenic region of this gene and the MYB gene has been identified to be a quantitative trait locus (QTL) controlling fetal hemoglobin level, and this region influnces erythrocyte, platelet, and monocyte counts as well as erythrocyte volume and hemoglobin content. DNA polymorphisms at this region associate with fetal hemoglobin levels and pain crises in sickle cell disease. A single nucleotide polymorphism in exon 1 of this gene is significantly associated with severity in beta-thalassemia/Hemoglobin E. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, May 2009]