mSin3A (SIN3A) (NM_001145358) Human Tagged ORF Clone

CAT#: RC227622

SIN3A (Myc-DDK-tagged)-Human SIN3 homolog A, transcription regulator (yeast) (SIN3A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIN3A
• Synonyms: WITKOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227622 representing NM_001145358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGCGTTTGGATGACCAGGAGTCACCGGTGTATGCAGCCCAGCAGCGTCGGATCCCTGGCAGCA
  CAGAGGCTTTTCCTCACCAGCACCGGGTGCTTGCCCCTGCCCCTCCTGTGTATGAAGCAGTGTCTGAGAC
  CATGCAGTCAGCTACGGGAATTCAGTACTCTGTAACACCCAGCTACCAGGTTTCAGCCATGCCACAGAGC
  TCCGGCAGTCATGGGCCCGCTATAGCAGCAGTTCATAGCAGCCATCATCACCCAACAGCGGTGCAGCCCC
  ACGGAGGCCAGGTGGTCCAGAGTCATGCTCATCCAGCCCCACCAGTTGCACCAGTGCAGGGACAGCAGCA
  ATTTCAGAGGCTGAAGGTGGAGGATGCGCTATCTTATCTTGACCAGGTGAAGCTGCAGTTTGGTAGTCAG
  CCTCAGGTCTACAATGATTTCCTTGACATCATGAAGGAATTTAAATCTCAGAGCATCGACACCCCAGGAG
  TGATTAGTCGTGTGTCCCAGCTATTCAAAGGCCACCCCGATCTGATAATGGGATTCAACACCTTCTTGCC
  CCCTGGCTACAAAATTGAGGTGCAAACCAATGACATGGTGAATGTGACAACTCCTGGCCAGGTTCATCAG
  ATTCCCACCCATGGCATCCAGCCACAGCCTCAACCACCACCCCAACATCCTTCCCAGCCTTCAGCCCAGT
  CAGCCCCAGCTCCTGCCCAGCCAGCTCCTCAGCCCCCACCTGCCAAAGTCAGCAAGCCCTCCCAACTGCA
  AGCACATACTCCGGCCAGTCAGCAGACTCCCCCACTTCCACCGTATGCATCCCCACGTTCTCCGCCGGTC
  CAGCCTCACACACCAGTGACAATCTCGTTGGGAACGGCCCCATCCTTGCAGAACAATCAACCTGTGGAGT
  TTAATCATGCCATCAACTATGTTAATAAGATCAAGAACAGATTTCAGGGCCAACCAGACATCTACAAAGC
  ATTCCTGGAGATTTTGCACACATATCAGAAAGAGCAGAGAAATGCCAAGGAAGCTGGAGGAAACTACACT
  CCAGCATTGACAGAGCAGGAGGTGTATGCCCAGGTTGCTCGTCTCTTTAAAAACCAGGAAGATTTGTTGT
  CAGAGTTTGGACAATTCCTACCAGATGCCAACAGCTCCGTGCTTTTAAGCAAAACAACTGCTGAGAAGGT
  TGATTCTGTGAGAAATGATCATGGAGGCACTGTCAAGAAGCCCCAACTGAACAACAAGCCGCAGAGGCCC
  AGCCAGAATGGCTGCCAGATCCGCAGACATCCTACAGGAACCACCCCTCCAGTTAAGAAGAAACCCAAAC
  TGCTCAATCTGAAGGATTCTTCTATGGCAGATGCCAGCAAACATAGTGGTGGAACAGAATCGTTATTTTT
  TGATAAGGTCCGAAAGGCTCTTCGGAGTGCAGAAGCCTACGAAAATTTCCTACGCTGTCTTGTTATTTTT
  AACCAGGAGGTGATCTCTCGTGCTGAGCTTGTGCAACTAGTCTCTCCTTTCCTGGGGAAATTCCCTGAGT
  TGTTTAATTGGTTTAAAAACTTTCTGGGCTATAAGGAGTCTGTACATCTGGAAACTTATCCAAAGGAGCG
  AGCCACAGAGGGCATTGCTATGGAGATAGATTATGCTTCTTGTAAACGATTGGGCTCCAGCTATCGAGCC
  TTACCAAAGAGTTACCAGCAGCCCAAGTGTACAGGACGGACTCCTCTCTGTAAAGAGGTTTTAAATGATA
  CCTGGGTTTCCTTCCCTTCGTGGTCTGAGGACTCTACCTTTGTGAGTTCCAAGAAGACTCAATATGAAGA
  ACATATTTATCGTTGTGAAGATGAACGCTTTGAGCTTGATGTAGTTTTAGAGACCAATCTGGCAACAATC
  CGGGTTCTGGAAGCAATACAGAAGAAGCTTTCCCGCTTGTCTGCTGAAGAACAAGCCAAATTTCGCTTGG
  ACAACACCCTTGGGGGCACATCAGAAGTCATCCATAGAAAAGCACTCCAGAGGATATATGCTGATAAAGC
  AGCTGACATCATTGATGGTCTGAGAAAGAATCCCTCCATTGCTGTTCCAATTGTCCTTAAAAGGTTGAAG
  ATGAAAGAGGAAGAATGGCGAGAAGCTCAGAGAGGCTTTAACAAAGTATGGCGAGAACAAAATGAGAAAT
  ACTACTTGAAGTCTCTGGACCACCAGGGGATCAACTTTAAACAGAATGACACCAAGGTCCTGAGGTCTAA
  GAGCTTACTCAATGAGATTGAGAGTATCTATGATGAGAGGCAAGAGCAGGCTACGGAGGAGAATGCTGGT
  GTACCTGTTGGCCCACACCTCTCACTTGCGTATGAAGACAAACAAATACTGGAAGATGCTGCTGCTCTGA
  TTATCCACCATGTGAAGAGGCAGACAGGCATTCAGAAGGAGGACAAATATAAGATAAAACAAATCATGCA
  TCATTTTATTCCAGATTTGCTCTTTGCCCAAAGAGGTGATCTCTCAGATGTGGAGGAAGAGGAAGAAGAA
  GAGATGGATGTAGATGAAGCCACAGGGGCAGTTAAGAAGCACAATGGTGTTGGGGGCAGTCCCCCTAAGT
  CCAAGTTACTGTTTAGTAACACAGCAGCTCAAAAATTAAGAGGAATGGATGAAGTATACAACCTCTTCTA
  TGTCAACAACAACTGGTATATTTTTATGCGACTGCACCAGATTCTCTGCCTGAGGCTGCTACGGATTTGT
  TCCCAAGCCGAACGGCAAATTGAAGAAGAAAACCGAGAGAGAGAATGGGAACGGGAAGTGCTGGGCATAA
  AGCGAGACAAGAGTGACAGCCCTGCCATTCAGCTACGTCTCAAAGAACCTATGGATGTTGATGTAGAAGA
  TTATTACCCAGCTTTCCTGGACATGGTGCGGAGCCTGCTGGATGGCAACATAGACTCATCACAGTATGAA
  GATTCACTGAGAGAGATGTTCACCATTCATGCCTACATTGCCTTTACCATGGACAAACTGATCCAGAGCA
  TTGTCAGACAGCTGCAGCATATCGTGAGTGATGAGATCTGTGTGCAGGTGACTGACCTTTACCTGGCAGA
  AAATAATAATGGGGCCACCGGAGGCCAGCTGAACACACAGAACTCAAGGAGCCTCCTGGAGTCAACGTAT
  CAGCGGAAAGCTGAGCAGCTAATGTCAGATGAGAATTGCTTTAAGCTTATGTTTATTCAGAGCCAAGGCC
  AGGTCCAGCTGACTATTGAGCTTCTGGACACAGAAGAGGAGAATTCGGATGACCCTGTGGAAGCAGAGCG
  CTGGTCAGACTACGTGGAGCGATACATGAATTCAGATACTACCTCGCCTGAGCTTCGTGAACATCTAGCA
  CAGAAACCAGTATTTCTCCCCAGGAATCTACGGCGGATCCGGAAGTGTCAACGTGGTCGAGAGCAGCAGG
  AAAAGGAAGGGAAGGAAGGAAACAGCAAGAAGACCATGGAGAATGTGGATAGTCTGGATAAGCTGGAGTG
  TAGATTCAAGCTGAATTCCTACAAGATGGTGTATGTGATCAAATCAGAGGACTATATGTATCGGAGGACC
  GCCCTGCTCCGGGCTCATCAGTCCCATGAGCGTGTAAGCAAGCGTCTACATCAGAGATTCCAGGCCTGGG
  TAGATAAATGGACCAAGGAGCATGTGCCCCGTGAAATGGCAGCAGAGACCAGCAAGTGGCTCATGGGTGA
  GGGGCTGGAGGGCCTGGTGCCCTGTACCACCACCTGTGATACAGAGACCCTGCATTTTGTGAGCATTAAC
  AAGTATCGTGTCAAATACGGCACAGTATTCAAAGCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227622 representing NM_001145358
  Red=Cloning site Green=Tags(s)
  
  MKRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSATGIQYSVTPSYQVSAMPQS
  SGSHGPAIAAVHSSHHHPTAVQPHGGQVVQSHAHPAPPVAPVQGQQQFQRLKVEDALSYLDQVKLQFGSQ
  PQVYNDFLDIMKEFKSQSIDTPGVISRVSQLFKGHPDLIMGFNTFLPPGYKIEVQTNDMVNVTTPGQVHQ
  IPTHGIQPQPQPPPQHPSQPSAQSAPAPAQPAPQPPPAKVSKPSQLQAHTPASQQTPPLPPYASPRSPPV
  QPHTPVTISLGTAPSLQNNQPVEFNHAINYVNKIKNRFQGQPDIYKAFLEILHTYQKEQRNAKEAGGNYT
  PALTEQEVYAQVARLFKNQEDLLSEFGQFLPDANSSVLLSKTTAEKVDSVRNDHGGTVKKPQLNNKPQRP
  SQNGCQIRRHPTGTTPPVKKKPKLLNLKDSSMADASKHSGGTESLFFDKVRKALRSAEAYENFLRCLVIF
  NQEVISRAELVQLVSPFLGKFPELFNWFKNFLGYKESVHLETYPKERATEGIAMEIDYASCKRLGSSYRA
  LPKSYQQPKCTGRTPLCKEVLNDTWVSFPSWSEDSTFVSSKKTQYEEHIYRCEDERFELDVVLETNLATI
  RVLEAIQKKLSRLSAEEQAKFRLDNTLGGTSEVIHRKALQRIYADKAADIIDGLRKNPSIAVPIVLKRLK
  MKEEEWREAQRGFNKVWREQNEKYYLKSLDHQGINFKQNDTKVLRSKSLLNEIESIYDERQEQATEENAG
  VPVGPHLSLAYEDKQILEDAAALIIHHVKRQTGIQKEDKYKIKQIMHHFIPDLLFAQRGDLSDVEEEEEE
  EMDVDEATGAVKKHNGVGGSPPKSKLLFSNTAAQKLRGMDEVYNLFYVNNNWYIFMRLHQILCLRLLRIC
  SQAERQIEEENREREWEREVLGIKRDKSDSPAIQLRLKEPMDVDVEDYYPAFLDMVRSLLDGNIDSSQYE
  DSLREMFTIHAYIAFTMDKLIQSIVRQLQHIVSDEICVQVTDLYLAENNNGATGGQLNTQNSRSLLESTY
  QRKAEQLMSDENCFKLMFIQSQGQVQLTIELLDTEEENSDDPVEAERWSDYVERYMNSDTTSPELREHLA
  QKPVFLPRNLRRIRKCQRGREQQEKEGKEGNSKKTMENVDSLDKLECRFKLNSYKMVYVIKSEDYMYRRT
  ALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSIN
  KYRVKYGTVFKAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145358
• ORF Size: 3819 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145358.1, NP_001138830.1
• RefSeq ORF: 3822 bp
• Locus ID: 25942
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Huntington's disease
• MW: 145 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]