SMAD3 (NM_001145104) Human Tagged ORF Clone
CAT#: RC227712L3
- LentiORF®
Lenti-ORF clone of SMAD3 (Myc-DDK-tagged)-Human SMAD family member 3 (SMAD3), transcript variant 4
"NM_001145104" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SMAD3 |
Synonyms | HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC227712).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001145104 |
ORF Size | 1278 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145104.1, NP_001138576.1 |
RefSeq Size | 5441 bp |
RefSeq ORF | 693 bp |
Locus ID | 4088 |
Cytogenetics | 15q22.33 |
Protein Families | Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors |
Protein Pathways | Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
MW | 48.1 kDa |
Gene Summary | 'The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. It also functions as a tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, Nov 2019]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC324817 | SMAD3 (untagged)-Human SMAD family member 3 (SMAD3), transcript variant 4 |
USD 420.00 |
|
RC227712 | SMAD3 (Myc-DDK-tagged)-Human SMAD family member 3 (SMAD3), transcript variant 4 |
USD 450.00 |
|
RG227712 | SMAD3 (GFP-tagged) - Human SMAD family member 3 (SMAD3), transcript variant 4 |
USD 500.00 |
|
RC227712L4 | Lenti-ORF clone of SMAD3 (mGFP-tagged)-Human SMAD family member 3 (SMAD3), transcript variant 4 |
USD 650.00 |
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