Metabotropic Glutamate Receptor 5 (GRM5) (NM_001143831) Human Tagged ORF Clone

CAT#: RC227736

GRM5 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 5 (GRM5), transcript variant a

Product Images

Western blot validation of overexpression lysate (Cat# LY428374) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227736 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRM5
• Synonyms: GPRC1E; mGlu5; MGLUR5; PPP1R86
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227736 representing NM_001143831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTTCTGTTGATCCTGTCAGTCTTACTTTTGAAAGAAGATGTCCGTGGGAGTGCACAGTCCAGTG
  AGAGGAGGGTGGTGGCTCACATGCCGGGTGACATCATTATTGGAGCTCTCTTTTCTGTTCATCACCAGCC
  TACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCGGTCCGTGAACAGTATGGCATTCAGAGAGTGGAG
  GCCATGCTGCATACCCTGGAAAGGATCAATTCAGACCCCACACTCTTGCCCAACATCACACTGGGCTGTG
  AGATAAGGGACTCCTGCTGGCATTCGGCTGTGGCCCTAGAGCAGAGCATTGAGTTCATAAGAGATTCCCT
  CATTTCTTCAGAAGAGGAAGAAGGCTTGGTACGCTGTGTGGATGGCTCCTCCTCTTCCTTCCGCTCCAAG
  AAGCCCATAGTAGGGGTCATTGGGCCTGGCTCCAGTTCTGTAGCCATTCAGGTCCAGAATTTGCTCCAGC
  TTTTCAACATACCTCAGATTGCTTACTCAGCAACCAGCATGGATCTGAGTGACAAGACTCTGTTCAAATA
  TTTCATGAGGGTTGTGCCTTCAGATGCTCAGCAGGCAAGGGCCATGGTGGACATAGTGAAGAGGTACAAC
  TGGACCTATGTATCAGCCGTGCACACAGAAGGCAACTATGGAGAAAGTGGGATGGAAGCCTTCAAAGATA
  TGTCAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAAATCTACAGTAATGCAGGGGAGCAGAGCTT
  TGATAAGCTGCTGAAGAAGCTCACAAGTCACTTGCCCAAGGCCCGGGTGGTGGCCTGCTTCTGTGAGGGC
  ATGACGGTGAGAGGTCTGCTGATGGCCATGAGGCGCCTGGGTCTAGCGGGAGAATTTCTGCTTCTGGGCA
  GTGATGGCTGGGCTGACAGGTATGATGTGACAGATGGATATCAGCGAGAAGCTGTTGGTGGCATCACAAT
  CAAGCTCCAATCTCCCGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCAGAAACAAACCAC
  CGAAACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGACTGGAAGGGTTTCCACAGGAGA
  ACAGCAAATACAACAAGACTTGCAATAGTTCTCTGACTCTGAAAACACATCATGTTCAGGATTCCAAAAT
  GGGATTTGTGATCAACGCCATCTATTCGATGGCCTATGGGCTCCACAACATGCAGATGTCCCTCTGCCCA
  GGCTATGCAGGACTCTGTGATGCCATGAAGCCAATTGATGGACGGAAACTTTTGGAGTCCCTGATGAAAA
  CCAATTTTACTGGGGTTTCTGGAGATACGATCCTATTCGATGAGAATGGAGACTCTCCAGGAAGGTATGA
  AATAATGAATTTCAAGGAAATGGGAAAAGATTACTTTGATTATATCAACGTTGGAAGTTGGGACAATGGA
  GAATTAAAAATGGATGATGATGAAGTATGGTCCAAGAAAAGCAACATCATCAGATCTGTGTGCAGTGAAC
  CATGTGAGAAAGGCCAGATCAAGGTGATCCGAAAGGGAGAAGTCAGCTGTTGTTGGACCTGTACACCTTG
  TAAGGAGAATGAGTATGTCTTTGATGAGTACACATGCAAGGCATGCCAACTGGGGTCTTGGCCCACTGAT
  GATCTCACAGGTTGTGACTTGATCCCAGTACAGTATCTTCGATGGGGTGACCCTGAACCCATTGCAGCTG
  TGGTGTTTGCCTGCCTTGGCCTCCTGGCCACCCTGTTTGTTACTGTAGTCTTCATCATTTACCGTGATAC
  ACCAGTAGTCAAGTCCTCAAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGCCTGGGCTACTTA
  TGTACCTTCTGCCTCATTGCGAAGCCCAAACAGATTTACTGCTACCTTCAGAGAATTGGCATTGGTCTCT
  CCCCAGCCATGAGCTACTCAGCCCTTGTAACAAAGACCAACCGTATTGCAAGGATCCTGGCTGGCAGCAA
  GAAGAAGATCTGTACCAAAAAGCCCAGATTCATGAGTGCCTGTGCCCAGCTAGTGATTGCTTTCATTCTC
  ATATGCATCCAGTTGGGCATCATCGTTGCCCTCTTTATAATGGAGCCTCCTGACATAATGCATGACTACC
  CAAGCATTCGAGAAGTCTACCTGATCTGTAACACCACCAACCTAGGAGTTGTCACTCCACTTGGATACAA
  TGGATTGTTGATTTTGAGCTGCACCTTCTATGCGTTCAAGACCAGAAATGTTCCAGCTAACTTCAACGAG
  GCCAAGTATATCGCCTTCACAATGTACACGACCTGCATTATATGGCTAGCTTTTGTGCCAATCTACTTTG
  GCAGCAACTACAAAATCATCACCATGTGTTTCTCGGTCAGCCTCAGTGCCACAGTGGCCCTAGGCTGCAT
  GTTTGTGCCGAAGGTGTACATCATCCTGGCCAAACCAGAGAGAAACGTGCGCAGCGCCTTCACCACATCT
  ACCGTGGTGCGCATGCATGTAGGGGATGGCAAGTCATCCTCCGCAGCCAGCAGATCCAGCAGCCTAGTCA
  ACCTGTGGAAGAGAAGGGGCTCCTCTGGGGAAACCTTAAGGTACAAAGACAGGAGACTGGCCCAGCACAA
  GTCGGAAATAGAGTGTTTCACCCCCAAAGGGAGTATGGGGAATGGTGGGAGAGCAACAATGAGCAGTTCC
  AATGGAAAATCCGTCACGTGGGCCCAGAATGAGAAGAGCAGCCGGGGGCAGCACCTGTGGCAGCGCCTGT
  CCATCCACATCAACAAGAAAGAAAACCCCAACCAAACGGCCGTCATCAAGCCCTTCCCCAAGAGCACGGA
  GAGCCGTGGCCTGGGCGCTGGCGCTGGCGCAGGCGGGAGCGCTGGGGGCGTGGGGGCCACGGGCGGTGCG
  GGCTGCGCAGGCGCCGGCCCAGGCGGGCCCGAGTCCCCAGACGCCGGCCCCAAGGCGCTGTATGATGTGG
  CCGAGGCTGAGGAGCACTTCCCGGCGCCCGCGCGGCCGCGCTCACCGTCGCCCATCAGCACGCTGAGCCA
  CCGCGCGGGCTCGGCCAGCCGCACGGACGACGATGTGCCGTCGCTGCACTCGGAGCCTGTGGCGCGCAGC
  AGCTCCTCGCAGGGCTCCCTCATGGAGCAGATCAGCAGTGTGGTCACCCGCTTCACGGCCAACATCAGCG
  AGCTCAACTCCATGATGCTGTCCACCGCGGCCCCCAGCCCCGGCGTCGGCGCCCCGCTCTGCTCGTCCTA
  CCTGATCCCCAAAGAGATCCAGTTGCCCACGACCATGACGACCTTTGCCGAAATCCAGCCTCTGCCGGCC
  ATCGAAGTCACGGGCGGCGCGCAGCCCGCGGCAGGGGCGCAGGCGGCTGGGGACGCGGCCCGGGAGAGCC
  CCGCGGCCGGTCCCGAGGCTGCGGCCGCCAAGCCAGACCTGGAGGAGCTGGTGGCTCTCACCCCGCCGTC
  CCCCTTCAGAGACTCGGTGGACTCGGGGAGCACAACCCCCAACTCGCCAGTGTCCGAGTCGGCCCTCTGT
  ATCCCGTCGTCTCCCAAATATGACACTCTTATCATAAGAGATTACACTCAGAGCTCCTCGTCGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227736 representing NM_001143831
  Red=Cloning site Green=Tags(s)
  
  MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVE
  AMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSSFRSK
  KPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYN
  WTYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEG
  MTVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNH
  RNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCP
  GYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNG
  ELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTD
  DLTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVVFIIYRDTPVVKSSSRELCYIILAGICLGYL
  CTFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFIL
  ICIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNE
  AKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTS
  TVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSS
  NGKSVTWAQNEKSSRGQHLWQRLSIHINKKENPNQTAVIKPFPKSTESRGLGAGAGAGGSAGGVGATGGA
  GCAGAGPGGPESPDAGPKALYDVAEAEEHFPAPARPRSPSPISTLSHRAGSASRTDDDVPSLHSEPVARS
  SSSQGSLMEQISSVVTRFTANISELNSMMLSTAAPSPGVGAPLCSSYLIPKEIQLPTTMTTFAEIQPLPA
  IEVTGGAQPAAGAQAAGDAARESPAAGPEAAAAKPDLEELVALTPPSPFRDSVDSGSTTPNSPVSESALC
  IPSSPKYDTLIIRDYTQSSSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143831
• ORF Size: 3636 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143831.1, NM_001143831.2, NP_001137303.1
• RefSeq ORF: 3639 bp
• Locus ID: 2915
• Cytogenetics: 11q14.2-q14.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Huntington's disease, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• MW: 132.47 kDa
• Gene Summary: 'This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'