EFTUD2 (NM_001142605) Human Tagged ORF Clone

CAT#: RC227789

EFTUD2 (Myc-DDK-tagged)-Human elongation factor Tu GTP binding domain containing 2 (EFTUD2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFTUD2
• Synonyms: MFDGA; MFDM; SNRNP116; Snrp116; Snu114; U5-116KD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227789 representing NM_001142605
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATGATGACGACGACGATGACGTAGGAGATCATGACGATGACCACCCTGGGATGGAGGTGGTGC
  TGCATGAGGACAAGAAGTACTACCCAACAGCCGAGGAGGTGTATGGTCCTGAGGTGGAGACCATAGTTCA
  AGAGGAAGACACTCAGCCTCTCACAGAACCCATTATTAAGCCAGTGAAAACCAAGAAATTCACTCTGATG
  GAGCAGACATTACCTGTTACGGTGTATGAGATGGATTTCTTGGCGGATCTGATGGATAACTCAGAGCTCA
  TCAGAAATGTGACCCTTTGTGGACATCTCCACCATGGCAAGACATGTTTTGTGGATTGTTTAATTGAACA
  GACTCACCCGGAAATCAGAAAGCGCTATGACCAAGATCTGTGCTATACTGACATCCTCTTCACAGAGCAA
  GAGAGAGGTGTAGGCATCAAAAGCACTCCTGTGACAGTGGTCTTGCCAGACACCAAAGGAAAATCTTATC
  TCTTCAATATCATGGACACTCCAGGACATGTGAATTTCTCTGATGAGGTCACAGCTGGCTTGCGCATCTC
  AGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGGGGGTGATGCTGAACACAGAGCGGCTGATCAAGCAT
  GCGGTGCAGGAGAGGCTGGCAGTCACTGTGTGCATCAACAAGATTGACCGGCTGATCCTGGAGCTGAAGC
  TGCCTCCAACTGATGCTTATTACAAGCTGCGCCACATTGTGGATGAGGTCAATGGATTAATAAGCATGTA
  TTCCACTGATGAGAACCTGATCCTTTCCCCACTCCTGGGTAACGTCTGCTTCTCCAGCTCCCAGTACAGC
  ATCTGCTTCACGCTGGGCTCCTTTGCCAAGATCTATGCCGACACCTTTGGTGACATTAATTACCAAGAAT
  TTGCTAAAAGACTCTGGGGTGACATCTACTTCAACCCTAAGACGCGAAAGTTCACCAAAAAGGCCCCAAC
  TAGCAGCTCCCAGAGAAGTTTCGTGGAGTTTATCTTGGAGCCTCTTTATAAGATCCTCGCCCAGGTTGTA
  GGTGACGTGGACACCAGCCTCCCACGGACCCTAGACGAGCTTGGCATCCACCTGACGAAGGAGGAGCTGA
  AGCTGAACATCCGCCCCTTGCTCAGGCTGGTCTGCAAAAAGTTCTTTGGCGAGTTCACAGGCTTTGTGGA
  CATGTGTGTGCAGCATATCCCTTCTCCAAAGGTGGGCGCCAAGCCCAAGATTGAGCACACCTACACCGGT
  GGTGTGGACTCCGACCTCGGCGAGGCTATGAGTGACTGTGACCCTGATGGCCCCCTGATGTGCCACACTA
  CTAAGATGTACAGCACAGATGATGGAGTCCAGTTTCACGCCTTTGGCCGGGTGCTGAGTGGCACCATTCA
  TGCTGGGCAGCCTGTGAAGGTACTGGGGGAGAACTACACCCTGGAGGATGAGGAAGACTCCCAGATATGC
  ACCGTGGGCCGCCTTTGGATCTCTGTGGCCAGGTACCACATCGAGGTGAACCGTGTTCCTGCTGGCAACT
  GGGTTCTGATTGAAGGTGTTGATCAACCAATTGTGAAGACAGCAACCATAACCGAACCCCGAGGCAATGA
  GGAGGCTCAGATTTTCCGACCCTTGAAGTTCAATACCACATCTGTTATCAAGATTGCTGTGGAGCCAGTC
  AACCCCTCAGAGCTGCCCAAGATGCTTGATGGCCTGCGCAAGGTCAACAAGAGCTATCCATCCCTCACCA
  CCAAGGTGGAGGAGTCTGGCGAGCATGTGATCCTGGGCACTGGGGAGCTCTACCTGGACTGTGTGATGCA
  TGATTTGCGGAAGATGTACTCAGAGATAGACATCAAGGTGGCTGACCCAGTTGTCACGTTTTGTGAGACG
  GTGGTGGAAACATCCTCCCTCAAGTGCTTTGCTGAAACGCCTAATAAGAAGAACAAGATCACCATGATTG
  CTGAGCCTCTTGAGAAGGGCCTGGCAGAGGACATAGAGAATGAGGTGGTCCAGATTACGTGGAACAGGAA
  GAAGCTGGGAGAGTTCTTCCAGACCAAGTACGATTGGGATCTGCTGGCTGCCCGTTCCATCTGGGCTTTT
  GGCCCTGATGCGACTGGCCCCAACATTCTGGTGGATGATACTCTGCCCTCTGAGGTGGACAAGGCTCTTC
  TTGGTTCAGTGAAGGACAGCATCGTTCAAGGTTTCCAGTGGGGAACCAGGGAGGGCCCCCTCTGTGATGA
  ATTGATTCGGAATGTCAAGTTTAAGATCCTGGATGCGGTGGTTGCCCAGGAGCCCCTGCACCGGGGCGGG
  GGCCAGATCATCCCCACAGCCAGGAGAGTCGTCTACTCTGCCTTCCTCATGGCTACTCCTCGTCTGATGG
  AGCCTTACTACTTTGTAGAGGTCCAGGCCCCTGCAGATTGCGTCTCTGCAGTTTATACCGTCCTGGCCAG
  GCGCAGGGGGCACGTGACTCAGGATGCACCCATCCCAGGCTCCCCTCTGTACACCATCAAAGCTTTTATC
  CCGGCCATCGACTCTTTTGGCTTTGAGACTGATCTCCGGACTCACACCCAGGGACAAGCCTTTTCTCTGT
  CTGTCTTCCACCACTGGCAGATTGTGCCTGGTGATCCCCTGGACAAGAGCATTGTCATCCGCCCCTTGGA
  GCCACAGCCAGCTCCTCACCTGGCCCGGGAATTCATGATCAAAACCCGCCGTAGGAAGGGCCTCAGTGAA
  GATGTGAGCATCAGCAAATTCTTCGATGATCCTATGTTGCTGGAACTTGCCAAACAGGATGTTGTGCTCA
  ATTACCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227789 representing NM_001142605
  Red=Cloning site Green=Tags(s)
  
  MDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLM
  EQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQ
  ERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKH
  AVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYS
  ICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVV
  GDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTG
  GVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQIC
  TVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPV
  NPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCET
  VVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAF
  GPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGG
  GQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFI
  PAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSE
  DVSISKFFDDPMLLELAKQDVVLNYPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142605
• ORF Size: 2811 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142605.1, NP_001136077.1
• RefSeq Size: 4058
• RefSeq ORF: 2814
• Locus ID: 9343
• Protein Pathways: Spliceosome
• MW: 105.4 kDa
• Gene Summary: This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]