TLE2 (NM_001144761) Human Tagged ORF Clone

CAT#: RC227876

TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227876 representing NM_001144761
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAATCACGGCTCACTGCAACCTCCGCCTCCCAAGATTCTCCTGCTTCAGGATTACAGACCCCGC
  TCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGATCTGCGACCGCATCAAAGAAGAATTCCAGTT
  TCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGAGAAGCTGGCCAGCGAGAAGACGGAAATGCAG
  CGACATTATGTCATGTATTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGGAGATTG
  TGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCAGGTGCT
  CCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGGGCAGCAGCAGCTC
  CAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCCCGCCCAGCCGGGCTGGTGGGCGGCAGTG
  CTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCCAGGCTCAGCTGGCGGCGGCTGTCAAGGAGGA
  CCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAGAGCCCCGAGCAGGAGTGCATCTCCCTCGCCC
  CCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGTGGTGGCGGGAAGCAGAGAGCAGATGAGA
  AGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTGATTACAATCTGGTGGTGGACGAGGACCA
  ACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAAGGTACCCATCTGCATTCCTGCCCGTCGG
  GACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGCTCACCGCTGCCTAGAGCCAAGGAGCTCA
  TCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCTGTGACTCCTCCCCGCCCCAGGACGCTTC
  CACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGCTGCCAAGCCAGCACCTTCCACGGACAGC
  GTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACCACGTCCTTCAGCCTGGGCTCCCACAGCA
  CTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCCTCCACCTGTCCCCCCAGGTCAGCAGCTC
  TGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCATCCCCATCTCCGAGGGTCATCCGTCTCT
  TCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCCTTCCACGTGTCTGCGGACGGGCAGATGC
  AGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCATCCCGCGGCACGCCCGGCAGCTGCACAC
  GCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGGCTCCACACAGCATGTGTACACGGGCGGC
  AAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCCAAGACGCCCGTGGCCCAGCTCGACTGCC
  TGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGGATGGCCGGAGTCTGATCGTGGGCGGTGA
  GGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCCCCGTATCAAGGCCGAGCTGACTTCCTCA
  GCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAGGTTTGCTTCTCCTGCTGCAGCGATGGCA
  ACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGCAGTTCCAGGGCCACACGGACGGCGCCAG
  CTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGGGGGCCTGGACAACACGGTGCGCTGCTGG
  GACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGCTCCCAGATTTTCTCCCTGGGCCACTGCC
  CTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACGTGGAGATCCTGCACGTCCGCAAGCCGGA
  GAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCTGAAGTTTGCCTCCTGCGTCCAAGGAGTC
  GTCCTCAGTCCTGAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227876 representing NM_001144761
  Red=Cloning site Green=Tags(s)
  
  MVQSRLTATSASQDSPASGLQTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQ
  RHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQL
  QPLSHHAPPVPLTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSP
  PESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARR
  DLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDS
  VALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVS
  SSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGG
  KGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSS
  APACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCW
  DLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCVQGV
  VLSPEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144761
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144761.1, NP_001138233.1
• RefSeq ORF: 2121 bp
• Locus ID: 7089
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 75.2 kDa
• Gene Summary: ''