CASKIN2 (NM_001142643) Human Tagged ORF Clone

CAT#: RC227932

CASKIN2 (Myc-DDK-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428227) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227932 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CASKIN2
• Synonyms: ANKS5B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227932 representing NM_001142643
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCCGCTGCACTACGCAGCCTGGCAGGGCCGGCTGGAGCCTGTGAGGCTGCTGCTGCGCGCCTCTG
  CGGCTGTCAATGCCGCCTCGCTGGACGGACAGATCCCCCTGCACCTGGCTGCACAGTATGGACATTATGA
  GGTGTCAGAAATGCTCCTCCAGCATCAGTCCAACCCATGCCTGGTCAACAAGGCCAAGAAGACGCCCCTG
  GACCTGGCCTGTGAATTTGGCCGACTCAAGGTGGCCCAGCTGCTTCTGAACAGCCACTTATGTGTGGCAC
  TGCTGGAGGGTGAGGCCAAAGACCCGTGTGACCCCAACTACACCACGCCCCTGCACTTGGCTGCCAAGAA
  TGGCCACAGAGAAGTCATCAGGCAGCTCCTGAGAGCTGGGATCGAGATCAACCGCCAGACCAAGACGGGT
  ACGGCGCTCCACGAGGCCGCACTGTATGGCAAGACCGAGGTGGTGCGGCTGCTTCTGGAGGGAGGTGTGG
  ACGTGAACATCCGGAATACGTATAACCAGACGGCGCTGGACATAGTGAATCAGTTCACCACCTCCCAGGC
  CAGCCGGGAAATCAAGCAGCTACTGCGGGAGGCCTCAGGGATCCTGAAGGTCCGAGCGCTCAAGGATTTC
  TGGAACCTCCACGATCCCACTGCTCTCAATGTCCGGGCAGGGGATGTCATCACGGTGCTAGAACAGCATC
  CCGACGGCCGCTGGAAGGGCCACATCCACGAGAGCCAGAGGGGCACAGACCGCATAGGCTACTTCCCCCC
  GGGCATTGTCGAGGTGGTCAGCAAGCGGGTGGGCATCCCTGCAGCCCGCCTCCCCTCCGCACCCACCCCC
  CTGCGCCCAGGCTTCTCCCGGACACCGCAGCCTCCTGCCGAAGAACCCCCGCACCCTCTTACCTACAGCC
  AGCTTCCTCGGGTGGGCCTCAGCCCAGACAGCCCAGCAGGTGACAGGAATAGTGTGGGCAGTGAGGGCAG
  CGTGGGCAGCATCCGCAGTGCCGGCAGCGGGCAGAGCTCTGAGGGCACTAATGGCCATGGCCCTGGCCTC
  CTGATTGAGAACGCCCAGCCACTGCCCTCTGCTGGAGAGGACCAGGTGCTGCCAGGACTCCACCCGCCGT
  CCCTGGCAGACAACCTGAGCCACCGCCCTCTGGCCAACTGCCGCTCTGGGGAGCAGATCTTCACCCAGGA
  CGTGCGGCCAGAACAGCTGCTGGAGGGGAAGGACGCGCAGGCCATTCATAACTGGCTAAGCGAGTTCCAG
  CTGGAGGGCTACACTGCCCACTTTCTGCAGGCCGGCTATGATGTGCCTACCATCAGCCGCATGACACCTG
  AGGACCTGACGGCCATCGGGGTGACCAAGCCTGGGCACAGGAAGAAGATCGCCTCAGAGATCGCTCAGCT
  CAGCATCGCCGAGTGGCTGCCCAGCTACATCCCAACGGACCTGCTGGAGTGGCTGTGTGCACTGGGGCTG
  CCACAGTACCACAAGCAGCTGGTGAGCAGCGGCTACGACTCCATGGGGCTGGTGGCCGACCTCACCTGGG
  AGGAGCTGCAGGAGATTGGGGTCAACAAGCTCGGGCATCAGAAGAAGCTCATGCTGGGGGTGAAGCGGCT
  GGCGGAGCTTCGGCGGGGCCTGCTGCAGGGGGAGGCCCTCAGCGAAGGCGGGCGCCGGCTGGCCAAGGGT
  CCGGAGCTGATGGCCATCGAGGGACTGGAGAACGGAGAAGGCCCAGCTACAGCTGGCCCACGGCTCCTCA
  CCTTCCAGGGCAGCGAACTAAGCCCAGAGCTACAGGCGGCCATGGCAGGGGGTGGCCCTGAACCACTCCC
  CCTCCCACCTGCCCGCTCTCCCAGCCAGGAGAGCATCGGGGCACGCTCACGGGGGTCTGGCCACTCACAG
  GAACAGCCTGCCCCACAGCCCAGCGGTGGAGATCCCAGCCCCCCCCAGGAGAGGAACCTCCCAGAGGGCA
  CAGAGCGGCCCCCTAAGCTTTGTTCTTCACTTCCTGGCCAAGGACCCCCACCCTATGTTTTTATGTACCC
  CCAGGGCTCACCCTCTAGCCCGGCCCCAGGGCCACCTCCTGGCGCACCCTGGGCCTTCTCCTACTTGGCC
  GGGCCCCCTGCCACTCCCCCAGACCCGCCTCGACCTAAGCGCCGGTCCCACAGCCTAAGCCGCCCTGGCC
  CCACAGAGGGGGATGCTGAGGGGGAGGCCGAAGGGCCAGTGGGCAGCACCCTAGGCAGTTATGCTACCCT
  TACCCGGCGGCCAGGACGCAGTGCCCTTGTCCGGACCAGTCCTAGTGTGACCCCAACCCCAGCTCGGGGG
  ACTCCTCGCAGCCAGTCCTTTGCCCTGCGGGCCCGGCGCAAAGGCCCCCCGCCCCCGCCCCCCAAGCGCC
  TCAGCTCCGTCTCTGGCCCCAGCCCGGAGCCACCTCCACTAGATGAGAGCCCAGGGCCCAAGGAAGGGGC
  CACAGGGCCCCGAAGGCGAACACTGAGTGAACCTGCTGGCCCCTCAGAGCCCCCTGGCCCACCTGCCCCG
  GCTGGGCCCGCGTCAGACACGGAGGAGGAGGAGCCAGGCCCTGAGGGGACGCCCCCATCTCGGGGCAGCT
  CTGGGGAAGGGCTGCCGTTTGCAGAGGAAGGGAACCTGACCATCAAACAGCGCCCGAAGCCCGCTGGCCC
  CCCGCCCCGAGAGACACCCGTGCCCCCCGGCCTCGATTTCAACCTCACGGAATCAGACACTGTTAAGCGG
  AGGCCCAAGTGCCGGGAGAGAGAGCCACTGCAGACCGCACTGCTGGCCTTCGGAGTGGCCAGTGCCACGC
  CTGGCCCCGCTGCCCCACTGCCTTCCCCAACTCCTGGCGAGTCTCCTCCAGCTTCTAGCCTTCCCCAGCC
  CGAGCCCAGCAGCCTTCCAGCCCAAGGAGTTCCAACCCCCCTTGCTCCCAGCCCCGCCATGCAGCCTCCA
  GTGCCGCCCTGCCCAGGGCCAGGTCTGGAAAGCTCAGCAGCTAGTCGGTGGAATGGGGAGACAGAACCCC
  CGGCCGCCCCTGCTGCCCTCCTCAAGGTGCCCGGAGCAGGAACAGCCCCCAAGCCTGTGTCGGTGGCCTG
  CACCCAGCTGGCATTTTCTGGCCCTAAGCTAGCGCCCCGGCTCGGCCCCCGCCCAGTGCCTCCTCCACGG
  CCTGAGAGCACTGGGACTGTGGGCCCAGGCCAGGCCCAGCAGAGACTGGAGCAGACCAGCTCGTCCCTGG
  CAGCTGCACTGAGAGCCGCAGAGAAGAGCATTGGCACCAAGGAGCAAGAGGGCACCCCCAGCGCCTCCAC
  CAAGCACATTCTGGATGACATCAGCACCATGTTCGACGCCCTGGCTGACCAGCTGGACGCCATGCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227932 representing NM_001142643
  Red=Cloning site Green=Tags(s)
  
  MRPLHYAAWQGRLEPVRLLLRASAAVNAASLDGQIPLHLAAQYGHYEVSEMLLQHQSNPCLVNKAKKTPL
  DLACEFGRLKVAQLLLNSHLCVALLEGEAKDPCDPNYTTPLHLAAKNGHREVIRQLLRAGIEINRQTKTG
  TALHEAALYGKTEVVRLLLEGGVDVNIRNTYNQTALDIVNQFTTSQASREIKQLLREASGILKVRALKDF
  WNLHDPTALNVRAGDVITVLEQHPDGRWKGHIHESQRGTDRIGYFPPGIVEVVSKRVGIPAARLPSAPTP
  LRPGFSRTPQPPAEEPPHPLTYSQLPRVGLSPDSPAGDRNSVGSEGSVGSIRSAGSGQSSEGTNGHGPGL
  LIENAQPLPSAGEDQVLPGLHPPSLADNLSHRPLANCRSGEQIFTQDVRPEQLLEGKDAQAIHNWLSEFQ
  LEGYTAHFLQAGYDVPTISRMTPEDLTAIGVTKPGHRKKIASEIAQLSIAEWLPSYIPTDLLEWLCALGL
  PQYHKQLVSSGYDSMGLVADLTWEELQEIGVNKLGHQKKLMLGVKRLAELRRGLLQGEALSEGGRRLAKG
  PELMAIEGLENGEGPATAGPRLLTFQGSELSPELQAAMAGGGPEPLPLPPARSPSQESIGARSRGSGHSQ
  EQPAPQPSGGDPSPPQERNLPEGTERPPKLCSSLPGQGPPPYVFMYPQGSPSSPAPGPPPGAPWAFSYLA
  GPPATPPDPPRPKRRSHSLSRPGPTEGDAEGEAEGPVGSTLGSYATLTRRPGRSALVRTSPSVTPTPARG
  TPRSQSFALRARRKGPPPPPPKRLSSVSGPSPEPPPLDESPGPKEGATGPRRRTLSEPAGPSEPPGPPAP
  AGPASDTEEEEPGPEGTPPSRGSSGEGLPFAEEGNLTIKQRPKPAGPPPRETPVPPGLDFNLTESDTVKR
  RPKCREREPLQTALLAFGVASATPGPAAPLPSPTPGESPPASSLPQPEPSSLPAQGVPTPLAPSPAMQPP
  VPPCPGPGLESSAASRWNGETEPPAAPAALLKVPGAGTAPKPVSVACTQLAFSGPKLAPRLGPRPVPPPR
  PESTGTVGPGQAQQRLEQTSSSLAAALRAAEKSIGTKEQEGTPSASTKHILDDISTMFDALADQLDAMLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142643
• ORF Size: 3360 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142643.1, NM_001142643.2, NP_001136115.1
• RefSeq Size: 4514
• RefSeq ORF: 3363
• Locus ID: 57513
• MW: 118.2 kDa
• Gene Summary: This gene encodes a large protein that contains six ankyrin repeats, as well as a Src homology 3 (SH3) domain and two sterile alpha motif (SAM) domains, which may be involved in protein-protein interactions. The C-terminal portion of this protein is proline-rich and contains a conserved region. A related protein interacts with calcium/calmodulin-dependent serine protein kinase (CASK). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]