CoCoA (CALCOCO1) (NM_001143682) Human Tagged ORF Clone

CAT#: RC227956

CALCOCO1 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY428318) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227956 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CALCOCO1
• Synonyms: calphoglin; Cocoa; PP13275
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227956 representing NM_001143682
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAATCACCACTAAGCCGGGCACCATCCCGTGGTGGAGTCAACTTTCTCAATGTAGCCCGGACCT
  ACATCCCCAACACCAAGGTGGAATGTCACTACACCCTTCCCCCAGGCACCATGCCCAGTGCCAGTGACTG
  GATTGGCATCTTCAAGGTGGAGGCTGCCTGTGTTCGGGATTACCACACATTTGTGTGGTCTTCCGTGCCT
  GAAAGTACAACTGATGGTTCCCCCATTCACACCAGTGTCCAGTTCCAAGAGCCAAGGCCCATGGATGAAC
  TGGTGACCCTGGAGGAGGCTGATGGGGGCTCTGACATCCTGCTGGTTGTCCCCAAGGCAACTGTGTTACA
  GAACCAGCTCGATGAGAGCCAGCAAGAACGGAATGACCTGATGCAGCTGAAGCTACAGCTGGAGGGACAG
  GTGACAGAGCTGAGGAGCCGAGTGCAGGAGCTCGAGAGGGCTCTGGCAACTGCCAGGCAGGAGCACACGG
  AGCTGATGGAACAGTACAAGGGGATTTCCCGGTCCCATGGGGAGATCACAGAAGAGAGGGACATCCTGAG
  CCGGCAACAGGGAGACCATGTGGCACGCATCCTGGAGCTAGAGGATGACATCCAGACCATCAGTGAGAAA
  GTGCTGACGAAGGAAGTGGAGCTGGACAGGCTTAGAGACACAGTGAAGGCCCTGACTCGGGAACAAGAGA
  AGCTCCTTGGGCAACTGAAAGAAGTACAAGCAGACAAGGAGCAAAGTGAGGCCGAGCTGGAGCCCTTGAA
  GGAGCAGCTTCGAGGGGCCCAGGAGCTTGCAGCCTCAAGCCAGCAGAAAGCCACCCTTCTTGGGGAGGAG
  TTGGCCAGTGCAGCAGCAGCCAGGGACCGCACCATAGCCGAACTACACCGCAGCCGCCTGGAAGTGGCTG
  AAGTTAACGGCAGGCTGGCTGAGCTCGGTTTGCACTTGAAGGAAGAAAAATGCCAATGGAGCAAGGAGCG
  GGCAGGGCTGCTGCAGAGTGTGGAGGCAGAGAAGGACAAGATCCTGAAGCTGAGTGCAGAGATACTTCGA
  TTGGAGAAGGCAGTTCAGGAGGAGAGGACCCAAAACCAAGTGTTCAAGACTGAGCTGGCCCGGGAGAAGG
  ATTCTAGCCTGGTACAGTTGTCAGAAAGTAAGCGGGAGCTGACAGAGCTGCGGTCAGCCCTGCGTGTGCT
  CCAGAAGGAAAAGGAGCAGTTACAGGAGGAGAAACAGGAATTGCTAGAGTACATGAGAAAGCTAGAGGCC
  CGCCTGGAGAAGGTGGCAGATGAGAAGTGGAATGAGGATGCCACCACAGAGGATGAGGAGGCCGCTGTGG
  GGCTGAGCTGCCCGGCAGCTCTGACAGACTCAGAGGACGAGTCCCCAGAAGACATGAGGCTCCCACCCTA
  TGGCCTTTGTGAGCGTGGAGACCCAGGCTCCTCTCCTGCTGGGCCTCGAGAGGCTTCTCCCCTTGTTGTC
  ATCAGCCAGCCGGCTCCCATTTCTCCTCACCTCTCTGGGCCAGCTGAGGACAGTAGCTCTGACTCGGAGG
  CTGAAGATGAGAAGTCAGTCCTGATGGCAGCTGTGCAGAGTGGGGGTGAGGAGGCCAACTTACTGCTTCC
  TGAACTGGGCAGTGCCTTCTATGACATGGCCAGTGGCTTTACAGTGGGTACCCTGTCAGAAACCAGCACT
  GGGGGCCCTGCCACCCCCACATGGAAGGAGTGTCCTATCTGTAAGGAGCGCTTTCCTGCTGAGAGTGACA
  AGGATGCCCTGGAGGACCACATGGATGGACACTTCTTTTTCAGCACCCAGGACCCCTTCACCTTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227956 representing NM_001143682
  Red=Cloning site Green=Tags(s)
  
  MEESPLSRAPSRGGVNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVP
  ESTTDGSPIHTSVQFQEPRPMDELVTLEEADGGSDILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQ
  VTELRSRVQELERALATARQEHTELMEQYKGISRSHGEITEERDILSRQQGDHVARILELEDDIQTISEK
  VLTKEVELDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELEPLKEQLRGAQELAASSQQKATLLGEE
  LASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVEAEKDKILKLSAEILR
  LEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEA
  RLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDPGSSPAGPREASPLVV
  ISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETST
  GGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143682
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143682.1, NP_001137154.1
• RefSeq ORF: 1821
• Locus ID: 57658
• Protein Families: Transcription Factors
• MW: 67.4 kDa