SH2D2A (NM_001161442) Human Tagged ORF Clone

CAT#: RC228298

SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY431326) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228298 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH2D2A
• Synonyms: F2771; SCAP; TSAD; VRAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228298 representing NM_001161442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCAAGCACCTTCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACTGCGG
  CATCTCCCCAGGCCCCGGAGGCTGCCTCCAACACAGGGAATGCTGAGAGGGCAGAGGAGGTGCCTGGAGA
  AGGAAGCCTGTTCCTGCAGGCCGAGACCCGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAG
  CACGGGGCAGCCCCTGCCTGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCA
  AGCCTCAGGGGTGCTACTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCG
  GACTTGCTGCCGCCACTTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGC
  GCCCACGCGCGGCTGCAGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAGACGC
  TCACCGAGCCCCTCGCCCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACCGAAGAATCAAACTT
  TGGAAGCAAAAGCCAGGACCCAAACCCCCAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCG
  ATGCAGAAAGAGGGGGCCGGGGAGAAGGAGCCCTCCCAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCA
  AACCTCAGCTGCCCCCAGAAGTCTACACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCC
  CTCCAATCCTATCTACAATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGG
  GAAGCCCCCAGCAACATCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCTGTCC
  TACGGAAGAGCTGGTCCAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAGCTGCATTCTGAGAA
  CTCTGTGATTGGGCAAGGCCCTCCCCTGCCCCACCAGCCCCCACCCGCCTGGAGACACACCCTCCCCCAC
  AATCTTTCTAGACAGGTGCTTCAGGACAGAGGACAGGCATGGCTTCCCCTTGGGCCTCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228298 representing NM_001161442
  Red=Cloning site Green=Tags(s)
  
  MSPSTFQITDMTRRSCQNLGYTAASPQAPEAASNTGNAERAEEVPGEGSLFLQAETRAWFQKTQAHWLLQ
  HGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRHFLLAQLRDGRHVVLGEDS
  AHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQDPNPQYSPIIKQGQAPVP
  MQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIYNEPDEPIAFYAMGRGSPG
  EAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQGPPLPHQPPPAWRHTLPH
  NLSRQVLQDRGQAWLPLGPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161442
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161442.1, NP_001154914.1
• RefSeq ORF: 1116
• Locus ID: 9047
• Protein Pathways: VEGF signaling pathway
• MW: 40.8 kDa
• Gene Summary: This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]