ZNF135 (NM_001164530) Human Tagged ORF Clone

CAT#: RC228316

ZNF135 (Myc-DDK-tagged)-Human zinc finger protein 135 (ZNF135), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF135
• Synonyms: pHZ-17; pT3; ZNF61; ZNF78L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228316 representing NM_001164530
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTAGGCTCGCGCCGGCGCAGTGTCGGCTGCCGGTGCCGCGGCCTTTGTCTCGCAGTCAGGAGGG
  AGCAAGTGACGTTTGAGGACGTGGTAGTGGGCTTCAGCCAGGAGGAGTGGGGGCAGCTGAAGCCTGCCCA
  GAGGACCCTGTACCGTGATGTAATGCTGGACACCTTCAGGCTTCTGGTCTCTGTGGGACATTGGTTACCG
  AAGCCGAATGTCATCTCCCTGCTGGAGCAAGAGGCAGAGCTGTGGGCGGTGGAGTCTAGACTTCCCCAAG
  GCGTGTACCCAGACTTGGAAACTAGACCCAAAGTCAAACTGTCAGTTCTAAAGCAAGGCATCTCTGAAGA
  AATATCCAACAGTGTCATCTTGGTAGAAAGATTCCTGTGGGATGGTCTGTGGTACTGCAGGGGTGAGGAC
  ACTGAGGGCCACTGGGAATGGAGTTGTGAGAGTCTAGAGAGCCTGGCAGTGCCGGTGGCCTTCACGCCTG
  TGAAGACGCCTGTTCTGGAGCAGTGGCAGAGGAATGGGTTTGGGGAAAACATAAGTCTGAACCCTGATCT
  CCCACATCAACCAATGACTCCTGAAAGACAAAGCCCCCACACATGGGGAACACGTGGAAAAAGGGAGAAG
  CCAGACCTAAATGTTTTACAGAAAACCTGTGTAAAAGAGAAACCCTACAAATGTCAGGAATGCGGAAAGG
  CCTTTAGTCACAGCTCAGCACTTATCGAACACCACCGGACGCACACAGGAGAGAGACCTTACGAATGTCA
  CGAATGCTTAAAAGGCTTCCGGAACAGCTCGGCACTTACCAAACACCAGAGAATCCATACTGGGGAGAAA
  CCCTATAAATGCACTCAGTGTGGGAGGACCTTCAACCAAATTGCCCCACTGATCCAGCACCAGAGAACTC
  ACACAGGTGAGAAGCCCTATGAATGCAGCGAATGTGGGAAATCCTTCAGTTTTAGGTCCTCCTTCAGCCA
  GCACGAGCGAACTCACACAGGCGAGAAGCCCTACGAGTGCAGTGAGTGTGGGAAAGCCTTCCGGCAAAGC
  ATCCACCTCACCCAGCATCTGCGAATCCACACTGGGGAGAAACCCTATCAGTGTGGTGAGTGTGGCAAGG
  CCTTTACCCACAGCTCCTCCCTTACCAAGCACCAGAGAACTCACACTGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228316 representing NM_001164530
  Red=Cloning site Green=Tags(s)
  
  MELGSRRRSVGCRCRGLCLAVRREQVTFEDVVVGFSQEEWGQLKPAQRTLYRDVMLDTFRLLVSVGHWLP
  KPNVISLLEQEAELWAVESRLPQGVYPDLETRPKVKLSVLKQGISEEISNSVILVERFLWDGLWYCRGED
  TEGHWEWSCESLESLAVPVAFTPVKTPVLEQWQRNGFGENISLNPDLPHQPMTPERQSPHTWGTRGKREK
  PDLNVLQKTCVKEKPYKCQECGKAFSHSSALIEHHRTHTGERPYECHECLKGFRNSSALTKHQRIHTGEK
  PYKCTQCGRTFNQIAPLIQHQRTHTGEKPYECSECGKSFSFRSSFSQHERTHTGEKPYECSECGKAFRQS
  IHLTQHLRIHTGEKPYQCGECGKAFTHSSSLTKHQRTHTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164530
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164530.1, NP_001158002.1
• RefSeq ORF: 1173 bp
• Locus ID: 7694
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 44.6 kDa
• Gene Summary: ''