GRAMD2B (NM_001146319) Human Tagged ORF Clone

CAT#: RC228359

GRAMD3 (Myc-DDK-tagged)-Human GRAM domain containing 3 (GRAMD3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY431387) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228359 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRAMD2B
• Synonyms: GRAMD3; NS3TP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228359 representing NM_001146319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGGAGACAGCAAGTCCTACAAGCAAAAAGGAGCATTCAGCAAACATTCCAAGCACAGCTTATGC
  CCTGGAAGAGTATGTTCCACGGGAGAGAAGTGAAGCCAGTGGGTCCGGACCTGGAACTCTCAGAGGCTGA
  GAATGGTGTGGAGGAGAAAAAGAAAGCCTGCAGGTCGCCAACAGCCCAATCCCCTACCCCATCTGTGGAG
  GCGGACTCCCCAGACCAGAAGAAAATCATTAGCCTATGGTCAAAATCCAGTTTTGATGGTGCCTCTTTAG
  CAAGTGATAAGAACGACTGTAAAACAGAAAGCAAAAATGACCCTAAGACTGAAAGAAAAAAGTCTTCATC
  TTCCAGCCAGTACAAGGCCAATATGCACTTTCACAAGTTGTTTCTTAGTGTCCCAACGGAGGAACCACTG
  AAGCAAAGCTTTACCTGTGCTCTACAGAAAGAAATACTATACCAAGGAAAGCTCTTTGTATCAGAAAACT
  GGATTTGTTTTCATTCCAAAGTCTTTGGAAAAGACACAAAGATCTCTATTCCAGCTTTCTCGGTAACCCT
  AATAAAGAAAACCAAAACTGCTCTTCTAGTGCCAAACGCCCTGATCATAGCAACAGTCACAGACAGGTAC
  ATATTTGTCTCCTTACTCTCCAGAGATTCAACTTACAAACTACTAAAATCTGTGTGTGGACACTTAGAAA
  ATACAAGTGTTGGTAACAGTCCCAATCCATCTTCTGCTGAAAACAGTTTCCGAGCAGACCGCCCTTCATC
  TCTGCCTCTGGATTTCAATGATGAATTCTCAGATCTGGATGGAGTGGTTCAACAAAGAAGGCAAGACATG
  GAAGGATATAGCAGTTCTGGTTCTCAAACTCCTGAATCTGAGAACTCTCGAGATTTCCATGCGACAGAAT
  CCCAAACAGTTCTGAATGTCTCCAAGGGAGAAGCAAAGCCAACTCGGGCAGATGCCCATGTGAACAGAGT
  ACCTGAAGGAAAAGCCAAGAGTCTCCCTGTACAGGGTCTGTCAGAAACTGTTGGAATCTTACATAAAGTC
  AAGTCTCAGAAATGTCCGATGCTTCACCATATTCTTATATTCTATGCAATTGTTGTCTGTGCACTAATCA
  TCTCGACCTTCTACATGAGATACAGAATTAATACTCTGGAGGAGCAGCTGGGGTTACTAACCTCCATTGT
  GGACACCCATAATACTGAACAGGCAGCACCATCTGGCCTGAGGTCACAAGTACAATTCAATGTGGAGGTT
  CTCTGTCAAGAGCTTACAGCTAACATAGTGAAATTAGAAAAGATACAAAATAACTTACAGAAGTTGCTTG
  AGAATGGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228359 representing NM_001146319
  Red=Cloning site Green=Tags(s)
  
  MTRRQQVLQAKRSIQQTFQAQLMPWKSMFHGREVKPVGPDLELSEAENGVEEKKKACRSPTAQSPTPSVE
  ADSPDQKKIISLWSKSSFDGASLASDKNDCKTESKNDPKTERKKSSSSSQYKANMHFHKLFLSVPTEEPL
  KQSFTCALQKEILYQGKLFVSENWICFHSKVFGKDTKISIPAFSVTLIKKTKTALLVPNALIIATVTDRY
  IFVSLLSRDSTYKLLKSVCGHLENTSVGNSPNPSSAENSFRADRPSSLPLDFNDEFSDLDGVVQQRRQDM
  EGYSSSGSQTPESENSRDFHATESQTVLNVSKGEAKPTRADAHVNRVPEGKAKSLPVQGLSETVGILHKV
  KSQKCPMLHHILIFYAIVVCALIISTFYMRYRINTLEEQLGLLTSIVDTHNTEQAAPSGLRSQVQFNVEV
  LCQELTANIVKLEKIQNNLQKLLENGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146319
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146319.1, NM_001146319.2, NP_001139791.1
• RefSeq ORF: 1344
• Locus ID: 65983
• Protein Families: Transmembrane
• MW: 49.7 kDa