RPAP3 (NM_001146076) Human Tagged ORF Clone

CAT#: RC228417

RPAP3 (Myc-DDK-tagged)-Human RNA polymerase II associated protein 3 (RPAP3), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY431445) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228417 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPAP3
• Synonyms: hSpagh; Tah1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228417 representing NM_001146076
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCGATCCATATAATCCCGTGTTGCCAACGAACAGAGCGTCAGCATATTTTAGACTGAAAAAAT
  TTGCTGTTGCTGAGTCTGATTGTAATTTAGCAGTTGCCTTGAATAGAAGTTATACAAAGGCTTATTCCAG
  ACGAGGTGCTGCTCGATTTGCTTTGCAAAAATTAGAAGAGGCCAAAAAAGATTATGAAAGAGTATTAGAA
  CTAGAACCAAATAACTTTGAAGCAACAAATGAACTCAGGAAAATCAGTCAGGCTTTAGCATCCAAAGAAA
  ACTCATATCCAAAGGAAGCTGACATAGTGATTAAGTCAACAGAAGGAGAGCGAAAGCAAATTGAAGCACA
  ACAGAATAAGCAGCAGGCCATTTCAGAGAAAGATCGGGGGAATGGATTTTTCAAAGAGGGGAAATATGAA
  AGAGCAATTGAATGCTATACTCGAGGGATAGCAGCAGATGGTGCTAATGCCCTTCTTCCAGCTAACAGAG
  CTATGGCCTATCTGAAGATTCAGAAATATGAAGAAGCTGAAAAAGACTGCACACAAGCCATTTTATTAGA
  TGGCTCATATTCTAAAGCTTTTGCCAGAAGAGGAACTGCAAGAACATTTTTGGGAAAGCTAAATGAGGCA
  AAACAAGATTTTGAAACTGTTTTACTTCTGGAACCTGGAAATAAGCAAGCAGTAACTGAACTCTCCAAAA
  TTAAAAAGGAATTAATTGAGAAAGGACACTGGGATGATGTCTTTCTTGATTCCACACAAAGACAAAATGT
  GGTAAAACCCATTGATAATCCACCGCATCCTGGATCAACTAAACCACTCAAGAAGGTTATTATTGAAGAA
  ACTGGTAATTTGATACAGACTATTGATGTGCCAGATAGCACTACTGCTGCTGCTCCAGAGAATAATCCTA
  TTAATCTAGCAAATGTAATAGCAGCCACAGGCACCACAAGTAAGAAGAATTCAAGCCAAGATGACCTTTT
  TCCCACAAGTGATACTCCAAGAGCAAAAGTATTGAAAATAGAAGAAGTCAGTGATACTTCATCCCTGCAA
  CCTCAAGCCAGTTTGAAGCAGGATGTATGTCAGTCTTACAGCGAGAAAATGCCCATAGAGATAGAACAAA
  AACCTGCTCAGTTTGCCACAACTGTTCTTCCTCCAATTCCTGCAAACTCGTTCCAGCTCGAATCTGATTT
  CAGACAATTGAAAAGTTCTCCAGATATGTTGTATCAGTATTTAAAGCAAATTGAACCATCTTTGTATCCT
  AAGTTGTTTCAGAAAAATCTGGATCCAGATGTATTCAACCAGATCGTTAAAATTCTGCATGACTTTTACA
  TTGAGAAAGAAAAGCCATTACTCATCTTTGAAATCTTACAAAGACTTTCTGAACTAAAAAGGTTTGATAT
  GGCAGTGATGTTTATGTCAGAAACAGAGAAAAAGATTGCACGTGCATTATTTAATCACATAGACAAGTCA
  GGATTGAAGGATAGTTCTGTCGAAGAACTCAAGAAAAGATACGGTGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228417 representing NM_001146076
  Red=Cloning site Green=Tags(s)
  
  MDADPYNPVLPTNRASAYFRLKKFAVAESDCNLAVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLE
  LEPNNFEATNELRKISQALASKENSYPKEADIVIKSTEGERKQIEAQQNKQQAISEKDRGNGFFKEGKYE
  RAIECYTRGIAADGANALLPANRAMAYLKIQKYEEAEKDCTQAILLDGSYSKAFARRGTARTFLGKLNEA
  KQDFETVLLLEPGNKQAVTELSKIKKELIEKGHWDDVFLDSTQRQNVVKPIDNPPHPGSTKPLKKVIIEE
  TGNLIQTIDVPDSTTAAAPENNPINLANVIAATGTTSKKNSSQDDLFPTSDTPRAKVLKIEEVSDTSSLQ
  PQASLKQDVCQSYSEKMPIEIEQKPAQFATTVLPPIPANSFQLESDFRQLKSSPDMLYQYLKQIEPSLYP
  KLFQKNLDPDVFNQIVKILHDFYIEKEKPLLIFEILQRLSELKRFDMAVMFMSETEKKIARALFNHIDKS
  GLKDSSVEELKKRYGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146076
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146076.1, NP_001139548.1
• RefSeq ORF: 1521
• Locus ID: 79657
• Protein Families: Protease
• MW: 56.9 kDa
• Gene Summary: This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]