PDE1B (NM_001165975) Human Tagged ORF Clone
CAT#: RC228422L3
- LentiORF®
Lenti-ORF clone of PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2
"NM_001165975" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PDE1B |
Synonyms | HEL-S-79p; PDE1B1; PDES1B |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC228422).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001165975 |
ORF Size | 1548 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001165975.1, NP_001159447.1 |
RefSeq ORF | 1551 bp |
Locus ID | 5153 |
Cytogenetics | 12q13.2 |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism |
MW | 58.9 kDa |
Gene Summary | 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC327057 | PDE1B (untagged)-Human phosphodiesterase 1B calmodulin-dependent (PDE1B) transcript variant 2 |
USD 880.00 |
|
RC228422 | PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
USD 470.00 |
|
RG228422 | PDE1B (GFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
USD 520.00 |
|
RC228422L4 | Lenti-ORF clone of PDE1B (mGFP-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2 |
USD 670.00 |
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