PDP1 (NM_001161780) Human Tagged ORF Clone

CAT#: RC228457

PDP1 (Myc-DDK-tagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2A; PPM2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228457 representing NM_001161780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCCAGTCAGAAGTTCCAGCCTGCCACTGTTCTCTG
  ATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATCTATGG
  CACTGCATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCTCAGAGTCGACTGAGA
  TACACACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACTGGTGGCAGTACACCCAAGGAA
  GGAGATATGCTTCCACACCACAGAAATTTTACCTCACACCTCCACAAGTCAATAGCATCCTTAAAGCTAA
  TGAATACAGTTTCAAAGTGCCAGAATTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAAT
  CAGCTGCCTGCAAATGCACCCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGC
  TTTTGGGGGTTTTTGATGGCCATGCAGGTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTTTATTA
  TATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGCGGCCGGGCA
  CTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATGATTACTTTAGTAAGGAGGCATCCAAATTGTACT
  TTAACAGCTTGAGGACTTACTGGCAAGAGCTTATAGACCTCAACACTGGTGAGTCGACTGATATTGATGT
  TAAGGAGGCTCTAATTAATGCCTTCAAGAGGCTTGATAATGACATCTCCTTGGAGGCGCAAGTTGGTGAT
  CCTAATTCTTTTCTCAACTACCTGGTGCTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATG
  TGGATGGTGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTGTGCAGGAAGAGGA
  CGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCACAATGCTCAAAATGAAAGAGAACTAGAACGGCTG
  AAATTGGAACATCCAAAGAGTGAGGCCAAGAGTGTCGTGAAACAGGATCGGCTGCTTGGCTTGCTGATGC
  CATTTAGGGCATTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAATCTGG
  CCCAGACCAGTTGAATGACAATGAATATACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTC
  ACTGCTGAGCCAGAGGTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTGTTGGCTACTGATG
  GGTTGTGGGAGACTATGCATAGGCAGGATGTGGTTAGGATTGTGGGTGAGTACCTAACTGGCATGCATCA
  CCAACAGCCAATAGCTGTTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGG
  AGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCATCTCATTCGCCACGCTGTGGGCA
  ACAACGAGTTTGGGACTGTTGATCATGAGCGCCTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCG
  AATGTACAGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCGTATCAAAAC
  CAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228457 representing NM_001161780
  Red=Cloning site Green=Tags(s)
  
  MCVCPGPRRIGIPVRSSSLPLFSDAMPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLR
  YTPHPAYATFCRPKENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSN
  QLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRA
  LLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGD
  PNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERL
  KLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYL
  TAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTER
  RTKMSSVFEDQNAATHLIRHAVGNNEFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQN
  QE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161780
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161780.1, NP_001155252.1
• RefSeq ORF: 1689
• Locus ID: 54704
• Protein Families: Druggable Genome, Phosphatase
• MW: 63.5 kDa
• Gene Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]