ARHGAP25 (NM_001166277) Human Tagged ORF Clone

CAT#: RC228497

ARHGAP25 (Myc-DDK-tagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY431525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228497 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP25
• Synonyms: HEL-S-308; KAIA0053
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228497 representing NM_001166277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAAAATTGCCAAGGAACTGGGATTTCAACCTGAAAGTGGAGGCTGCGAAAATAGCTCGGTCAA
  GGAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCC
  CATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTGCTG
  AGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTACCAG
  GATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGT
  GTTTGGCCAGCGCTTGGATGAGACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTG
  GTGGAGAAATGTGCAGAGTTCATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGC
  AGGACAACCTGGTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACAC
  AGATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTTCCCTGG
  AGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCTCAGCAGGAGT
  TGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGCAGGTTCCTACA
  TGAAATACAGCTGAACTGTGCTGTTAACAAGATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAAT
  CTCATCAGGTCGAAGGTCGAAGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGA
  CTATGATGATCAGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCA
  GAAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGACCTCCGA
  ATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGCCCCACCGGACAGCAGC
  CGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAAAAGCCAGGAGACTGGAAAATGCAATC
  TCGTAAAAGGACTCAAACACTCCCTAACCGGAAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGC
  AGCAAAATGGAGATCTTTAAAAATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACA
  GGAGAACGATGTCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCC
  TTCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAGGGAGAT
  ACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAAGAGGAAATTGATTCTT
  TGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAGAAGCAAATGTATGAGGAACAGATTAA
  AAACCTTGAGAAGGAAAATTATGACGTTTGGGCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAA
  AAGAAGAAGTCTGCAGCCCTAGAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGA
  GGAACAAGGCCTTGGAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228497 representing NM_001166277
  Red=Cloning site Green=Tags(s)
  
  MSLKLPRNWDFNLKVEAAKIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVL
  RAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPVFGQRLDETVAYEQKFGPHLVPIL
  VEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPW
  SQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVN
  LIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLR
  ISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANS
  SKMEIFKNEFWSPSSEAKAGEGHRRTMSQDLRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGD
  TLASPNSETGPGKKNSGEEEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKE
  KKKSAALEISLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166277
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166277.1, NP_001159749.1
• RefSeq ORF: 1821
• Locus ID: 9938
• MW: 68.8 kDa
• Gene Summary: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM, Mar 2008]