Semaphorin 7a (SEMA7A) (NM_001146029) Human Tagged ORF Clone

CAT#: RC228520

SEMA7A (Myc-DDK-tagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEMA7A
• Synonyms: CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228520 representing NM_001146029
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGCCCGCCGGCTC
  GGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCCGCCGCCTCCGCCCAGGGCCA
  CCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGCCATGTAGGGCAGGACCGGGTGGACTTTGGC
  CAGACTGAGCCGCACACGGTGCTTTTCCACGAGCCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCA
  AGGTCTACCTCTTTGACTTCCCCGAGGGCAAGAACGCATCTGTGCGCACGGACTGCGAGAACTACATCAC
  TCTCCTGGAGAGGCGGAGTGAGGGGCTGCTGGCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAAC
  CTGGTGAATGGCACTGTGGTGCCACTTGGCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACT
  CCCTGGTTCTGTTTGAAGGGGACGAGGTGTATTCCACCATCCGGAAGCAGGAATACAATGGGAAGATCCC
  TCGGTTCCGCCGCATCCGGGGCGAGAGTGAGCTGTACACCAGTGATACTGTCATGCAGAACCCACAGTTC
  ATCAAAGCCACCATCGTGCACCAAGACCAGGCTTACGATGACAAGATCTACTACTTCTTCCGAGAGGACA
  ATCCTGACAAGAATCCTGAGGCTCCTCTCAATGTGTCCCGTGTGGCCCAGTTGTGCAGGGGGGACCAGGG
  TGGGGAAAGTTCACTGTCAGTCTCCAAGTGGAACACTTTTCTGAAAGCCATGCTGGTATGCAGTGATGCT
  GCCACCAACAAGAACTTCAACAGGCTGCAAGACGTCTTCCTGCTCCCTGACCCCAGCGGCCAGTGGAGGG
  ACACCAGGGTCTATGGTGTTTTCTCCAACCCCTGGAACTACTCAGCCGTCTGTGTGTATTCCCTCGGTGA
  CATTGACAAGGTCTTCCGTACCTCCTCACTCAAGGGCTACCACTCAAGCCTTCCCAACCCGCGGCCTGGC
  AAGTGCCTCCCAGACCAGCAGCCGATACCCACAGAGACCTTCCAGGTGGCTGACCGTCACCCAGAGGTGG
  CGCAGAGGGTGGAGCCCATGGGGCCTCTGAAGACGCCATTGTTCCACTCTAAATACCACTACCAGAAAGT
  GGCCGTCCACCGCATGCAAGCCAGCCACGGGGAGACCTTTCATGTGCTTTACCTAACTACAGACAGGGGC
  ACTATCCACAAGGTGGTGGAACCGGGGGAGCAGGAGCACAGCTTCGCCTTCAACATCATGGAGATCCAGC
  CCTTCCGCCGCGCGGCTGCCATCCAGACCATGTCGCTGGATGCTGAGCGGAGGAAGCTGTATGTGAGCTC
  CCAGTGGGAGGTGAGCCAGGTGCCCCTGGACCTGTGTGAGGTCTATGGCGGGGGCTGCCACGGTTGCCTC
  ATGTCCCGAGACCCCTACTGCGGCTGGGACCAAGGCCGCTGCATCTCCATCTACAGCTCCGAACGGTCAG
  TGCTGCAATCCATTAATCCAGCCGAGCCACACAAGGAGTGTCCCAACCCCAAACCAGACAAGGCCCCACT
  GCAGAAGGTTTCCCTGGCCCCAAACTCTCGCTACTACCTGAGCTGCCCCATGGAATCCCGCCACGCCACC
  TACTCATGGCGCCACAAGGAGAACGTGGAGCAGAGCTGCGAACCTGGTCACCAGAGCCCCAACTGCATCC
  TGTTCATCGAGAACCTCACGGCGCAGCAGTACGGCCACTACTTCTGCGAGGCCCAGGAGGGCTCCTACTT
  CCGCGAGGCTCAGCACTGGCAGCTGCTGCCCGAGGACGGCATCATGGCCGAGCACCTGCTGGGTCATGCC
  TGTGCCCTGGCCGCCTCCCTCTGGCTGGGGGTGCTGCCCACACTCACTCTTGGCTTGCTGGTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228520 representing NM_001146029
  Red=Cloning site Green=Tags(s)
  
  MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFG
  QTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTDCENYITLLERRSEGLLACGTNARHPSCWN
  LVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQF
  IKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDA
  ATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPG
  KCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRG
  TIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCL
  MSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHAT
  YSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHA
  CALAASLWLGVLPTLTLGLLVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146029
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001146029.1, NM_001146029.2, NP_001139501.1
• RefSeq ORF: 1959
• Locus ID: 8482
• Protein Pathways: Axon guidance
• MW: 73.36 kDa
• Gene Summary: This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]