ARHGAP40 (NM_001164431) Human Tagged ORF Clone

CAT#: RC228530

ARHGAP40 (Myc-DDK-tagged)-Human Rho GTPase activating protein 40 (ARHGAP40)

Product Images

Western blot validation of overexpression lysate (Cat# LY431558) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228530 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP40
• Synonyms: C20orf95; dJ1100H13.4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228530 representing NM_001164431
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCCTGCCCTCCTCCCCGCCGCCCAGATGGAGAGGCTGGCCCCAGGGCCCCTAGCCTCACCGT
  GTCCTCGGATCCCGCGGGCCCGCATTGCCAGGCGCTGCGCCCAGCGCTGGGCCGACCTGGGCTGCAGCTC
  TGGCCCCTCCTCAGGCCGAATGGATCAGCTTCCCCAGAAGAATCTCCTGCGACTACACCCTGCTGGCTCT
  GCTGGCTGTTCCACTGGAGTGGAGAGCTCCAGCATGGATGGCTTTTGGATGGAGGTGGAACAGATCCAAC
  AGAGAGATGAGCTGAGGGAAGAGGACAGTGGCGGGAATGAAGGCCAGCTTCCAGAGGGGGAAGCTGAATC
  CCAGTGGCTGCAGGACACAGGCCTGTCGGGCCTCCTTGGTGGCCTGGGCTTGGATGGTGATCACCAGGAG
  CTCCTGTCCACCCTGACACAGACCCAGGTGGCCGCTGTGTGCCGCCGGCTGGACATCTATGCTCGCTCAG
  TGCGAAGACAACACAAGACACCTGTCAGAGATGTCAGGGATGTCTTTGGGGTCTTCAATTCAGGGAAAAT
  GTCGTCAGAGAATGGCGACTCCGGTATGAAGGGGGCCCAGCTCAGTTCCGGGGCCTCTAAGTTTCCTCCA
  GCAGCAGAGCCTGGGGGGCTGCAGGAGCAGGCTGGGAGGGAAGAAGCCTTCAACATGGACTCTGCCTACT
  CAGAACAAGCTGCGGTGCTCCTGCAGAGGAGCAGGCCATCCCGGGGAGGCACCTCTGCCTGGGGCAAGTG
  TTCCCTGCCGAAGTTTACCGTCCCCAAAGGCAGACTTGGCGTGACGAGGATAGGAGACTTGTCCCTGCAG
  GATATGAGGAAGGTACCTTCTCTGGCCCTCATAGAGCTGACCGCGCTCTGTGACATCCTCGGCTTGGACC
  TGAAGAGGAGCAAGGCGGGGAAGTGGAAAGCGGCAGAAACTCGCCTCTTTGGTGTGCCCCTTGACAGCCT
  GCTAGAAGCTGACCACAAAGTCCTCCCCAGCACACAGGTCCCGCTGGTCCTTCAAGCCCTGCTGTCCTGT
  TTGGAAAAGAGAGGACTGGACATGGAAGGCATTCTCAGGGTGCCCGGATCCCAGGCCAGGGTCAAGGGGC
  TGGAACAGAAACTGGAGAGAGACTTCTATGCTGGCCTTTTTAGCTGGGACGAGGTTCATCACAATGACGC
  CTCTGATTTGCTCAAAAGGTTCATCCGGAAGCTGCCGACACCTTTGCTCACGGCTGAGTACCTCCCGGCC
  TTCGCCGTGGTGCCTAACATCCCCAACCTGAAGCAGCGCCTGCAGGTTCTTCACCTGCTCATCCTCATCC
  TCCCAGAACCCAACAGAAATGCCTTAAAGGCACTGCTGGAATTCCTCAGGAAGGTGGTGGCCCGGGAACA
  GCACAACAAGATGACTCTGAGGAATGTTTCCACCGTGATGGCCCCTAACCTCTTTCTGCACCAAGGGCGG
  CCCCCCAAGCTCCCCAAAGGCAAGGAGAAGCAGCTGGCAGAAGGGGCAGCCGAGGTGGTGCAGATAATGG
  TGCACTACCAGGATCTGCTGTGGACGGTCGCCTCTTTCCTGGTCGCCCAGGTGCGAAAACTGAACGACAG
  TAGCAGCAGGCGCCCCCAGCTCTGCGACGCAGGCCTCAAGACTTGGCTGCGGAGGATGCACGCAGACAGG
  GACAAGGCGGGGGACGGCCTCGAGGCGACTCCCAAGGTGGCAAAGATCCAGGTGGTCTGGCCTATCAAGG
  ACCCCTTGAAGGTGCCTCTCACCCCCAGCACCAAAGTGGCCCACGTCCTGAGGCAGTTCACAGAGCACCT
  CAGCCCTGGTTCCAAGGGTCAAGAAGACAGTGAGGACATGGACAGCCTCCTTCTACACCATAGGTCCATG
  GAGTCAGCCAACATCCTCCTCTATGAAGTTGGAGGGAATATCAATGAGCATCGCCTGGACCCAGATGCCT
  ACCTCTTAGATCTGTACCGTGCCAACCCGCATGGTGAGTGGGTCCTTAAACAGAACCCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228530 representing NM_001164431
  Red=Cloning site Green=Tags(s)
  
  MAEPALLPAAQMERLAPGPLASPCPRIPRARIARRCAQRWADLGCSSGPSSGRMDQLPQKNLLRLHPAGS
  AGCSTGVESSSMDGFWMEVEQIQQRDELREEDSGGNEGQLPEGEAESQWLQDTGLSGLLGGLGLDGDHQE
  LLSTLTQTQVAAVCRRLDIYARSVRRQHKTPVRDVRDVFGVFNSGKMSSENGDSGMKGAQLSSGASKFPP
  AAEPGGLQEQAGREEAFNMDSAYSEQAAVLLQRSRPSRGGTSAWGKCSLPKFTVPKGRLGVTRIGDLSLQ
  DMRKVPSLALIELTALCDILGLDLKRSKAGKWKAAETRLFGVPLDSLLEADHKVLPSTQVPLVLQALLSC
  LEKRGLDMEGILRVPGSQARVKGLEQKLERDFYAGLFSWDEVHHNDASDLLKRFIRKLPTPLLTAEYLPA
  FAVVPNIPNLKQRLQVLHLLILILPEPNRNALKALLEFLRKVVAREQHNKMTLRNVSTVMAPNLFLHQGR
  PPKLPKGKEKQLAEGAAEVVQIMVHYQDLLWTVASFLVAQVRKLNDSSSRRPQLCDAGLKTWLRRMHADR
  DKAGDGLEATPKVAKIQVVWPIKDPLKVPLTPSTKVAHVLRQFTEHLSPGSKGQEDSEDMDSLLLHHRSM
  ESANILLYEVGGNINEHRLDPDAYLLDLYRANPHGEWVLKQNPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164431
• ORF Size: 2022 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164431.1, NP_001157903.1
• RefSeq ORF: 2025
• Locus ID: 343578
• MW: 74.2 kDa