ZNF135 (NM_007134) Human Tagged ORF Clone

CAT#: RC228534

ZNF135 (Myc-DDK-tagged)-Human zinc finger protein 135 (ZNF135), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF135
• Synonyms: pHZ-17; pT3; ZNF61; ZNF78L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228534 ORF sequence, codon optimized.
  Due to the complexity of NM_007134, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGGGAGTAGAAGACGCTCTGTGGGTTGCAGATGTCGGGGACTTTGCCTGGCGGTTCGCAGAG
  AACAGGTGACTTTTGAAGACGTCGTCGTGGGGTTTAGCCAAGAGGAGTGGGGTCAGCTTAAGCCCGCACA
  ACGCACACTGTACAGGGACGTAATGTTGGATACGTTCAGACTGCTCGTCTCCGTCGGTCACTGGCTGCCC
  AAGCCTAACGTGATCAGCCTCCTGGAGCAGGAAGCCGAGTTGTGGGCCGTGGAGTCACGACTCCCGCAGG
  GAGTATACCCCGAGATCAAGGGACATTTCCAGTTCCTGCTTCTGAGTGATCTGGAGACACGGCCAAAGGT
  AAAACTGAGCGTACTCAAGCAGGGCATCAGTGAAGAGATAAGCAACAGCGTTATCCTCGTGGAACGCTTT
  CTGTGGGACGGACTTTGGTATTGTCGGGGTGAAGATACGGAGGGGCATTGGGAATGGTCATGTGAGAGCC
  TCGAAAGTCTGGCCGTGCCGGTGGCATTTACCCCTGTGAAAACACCTGTGCTCGAACAGTGGCAGAGAAA
  TGGCTTCGGGGAAAATATCAGCCTCAACCCCGATCTGCCGCATCAGCCCATGACGCCAGAACGCCAGAGC
  CCACACACCTGGGGGACACGCGGGAAAAGAGAAAAACCAGACCTCAACGTACTGCAAAAGACATGTGTGA
  AAGAAAAACCCTATAAGTGCCAGGAGTGTGGAAAGGCATTCTCACACAGCTCCGCGTTGATCGAGCATCA
  CAGAACGCACACCGGGGAACGCCCGTACGAATGCCACGAATGTTTGAAAGGCTTTCGCAATAGCAGCGCC
  CTGACGAAGCACCAAAGAATCCATACAGGAGAGAAACCTTACAAATGCACACAGTGTGGCCGGACTTTTA
  ACCAGATAGCGCCTTTGATACAGCATCAGCGAACACATACTGGCGAGAAACCTTATGAGTGCTCTGAGTG
  CGGAAAGTCATTTTCATTCAGGTCATCCTTCTCACAACACGAGAGAACTCACACTGGCGAAAAGCCCTAC
  GAATGTAGTGAGTGCGGGAAAGCTTTTAGGCAATCTATACACCTGACCCAACACCTTAGGATCCACACTG
  GCGAGAAGCCTTACCAGTGCGGGGAATGTGGAAAAGCTTTCTCACACTCCTCTTCACTTACCAAGCATCA
  GAGGATTCACACTGGGGAAAAACCTTACGAATGTCATGAGTGCGGGAAAGCGTTCACCCAGATCACCCCT
  CTCATCCAGCATCAGCGGACACATACAGGCGAAAAGCCGTACGAGTGTGGAGAGTGCGGCAAAGCATTCA
  GCCAGTCTACACTGCTTACCGAGCACAGAAGGATTCACACCGGAGAGAAGCCATATGGGTGTAACGAATG
  TGGGAAAACATTCAGTCATAGTAGCTCCCTCAGCCAGCACGAACGAACTCATACCGGCGAGAAGCCCTAC
  GAATGCTCACAGTGCGGCAAAGCTTTCCGACAGAGCACTCATCTGACACAGCACCAGCGAATCCACACAG
  GCGAAAAACCATATGAATGCAACGACTGTGGCAAAGCATTTAGTCACTCCAGCAGCCTGACTAAACATCA
  ACGCATCCACACTGGGGAAAAACCGTATGAATGCAACCAGTGTGGTAGAGCCTTTAGTCAACTTGCACCA
  CTCATTCAGCACCAAAGAATTCACACAGGAGAAAAACCTTACGAATGCAATCAGTGTGGACGAGCATTCA
  GTCAGAGCTCTCTGCTCATAGAACATCAGCGAATACACACAAAGGAAAAACCTTACGGCTGCAATGAGTG
  TGGAAAAAGCTTTAGTCACTCCAGCTCACTCTCTCAGCATGAGCGGACCCACACCGGAGAGAAACCATAC
  GAGTGTCACGATTGTGGCAAAAGCTTCCGGCAGAGCACCCACCTGACCCAGCACCGCAGAATCCACACCG
  GTGAGAAACCATATGCATGTAGGGATTGCGGTAAAGCATTCACCCACTCATCCAGCCTTACTAAGCATCA
  AAGAACTCACACTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228534 representing NM_007134
  Red=Cloning site Green=Tags(s)
  
  MELGSRRRSVGCRCRGLCLAVRREQVTFEDVVVGFSQEEWGQLKPAQRTLYRDVMLDTFRLLVSVGHWLP
  KPNVISLLEQEAELWAVESRLPQGVYPEIKGHFQFLLLSDLETRPKVKLSVLKQGISEEISNSVILVERF
  LWDGLWYCRGEDTEGHWEWSCESLESLAVPVAFTPVKTPVLEQWQRNGFGENISLNPDLPHQPMTPERQS
  PHTWGTRGKREKPDLNVLQKTCVKEKPYKCQECGKAFSHSSALIEHHRTHTGERPYECHECLKGFRNSSA
  LTKHQRIHTGEKPYKCTQCGRTFNQIAPLIQHQRTHTGEKPYECSECGKSFSFRSSFSQHERTHTGEKPY
  ECSECGKAFRQSIHLTQHLRIHTGEKPYQCGECGKAFSHSSSLTKHQRIHTGEKPYECHECGKAFTQITP
  LIQHQRTHTGEKPYECGECGKAFSQSTLLTEHRRIHTGEKPYGCNECGKTFSHSSSLSQHERTHTGEKPY
  ECSQCGKAFRQSTHLTQHQRIHTGEKPYECNDCGKAFSHSSSLTKHQRIHTGEKPYECNQCGRAFSQLAP
  LIQHQRIHTGEKPYECNQCGRAFSQSSLLIEHQRIHTKEKPYGCNECGKSFSHSSSLSQHERTHTGEKPY
  ECHDCGKSFRQSTHLTQHRRIHTGEKPYACRDCGKAFTHSSSLTKHQRTHTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007134
• ORF Size: 2046 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007134.1, NP_009065.1
• RefSeq Size: 3346 bp
• RefSeq ORF: 2049 bp
• Locus ID: 7694
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 78.2 kDa
• Gene Summary: ''