epithelial Sodium Channel alpha (SCNN1A) (NM_001159575) Human Tagged ORF Clone
CAT#: RC228538
SCNN1A (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 3
"NM_001159575" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SCNN1A |
Synonyms | BESC2; ENaCa; ENaCalpha; LIDLS3; SCNEA; SCNN1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC228538 representing NM_001159575
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGTATCAAGGGGAACAAGCTGGAGGAGCAGGACCCTAGACCTCTGCAGCCCATACCAGGTCTCA TGGAGGGGAACAAGCTGGAGGAGCAGGACTCTAGCCCTCCACAGTCCACTCCAGGGCTCATGAAGGGGAA CAAGCGTGAGGAGCAGGGGCTGGGCCCCGAACCTGCGGCGCCCCAGCAGCCCACGGCGGAGGAGGAGGCC CTGATCGAGTTCCACCGCTCCTACCGAGAGCTCTTCGAGTTCTTCTGCAACAACACCACCATCCACGGCG CCATCCGCCTGGTGTGCTCCCAGCACAACCGCATGAAGACGGCCTTCTGGGCAGTGCTGTGGCTCTGCAC CTTTGGCATGATGTACTGGCAATTCGGCCTGCTTTTCGGAGAGTACTTCAGCTACCCCGTCAGCCTCAAC ATCAACCTCAACTCGGACAAGCTCGTCTTCCCCGCAGTGACCATCTGCACCCTCAATCCCTACAGGTACC CGGAAATTAAAGAGGAGCTGGAGGAGCTGGACCGCATCACAGAGCAGACGCTCTTTGACCTGTACAAATA CAGCTCCTTCACCACTCTCGTGGCCGGCTCCCGCAGCCGTCGCGACCTGCGGGGGACTCTGCCGCACCCC TTGCAGCGCCTGAGGGTCCCGCCCCCGCCTCACGGGGCCCGTCGAGCCCGTAGCGTGGCCTCCAGCTTGC GGGACAACAACCCCCAGGTGGACTGGAAGGACTGGAAGATCGGCTTCCAGCTGTGCAACCAGAACAAATC GGACTGCTTCTACCAGACATACTCATCAGGGGTGGATGCGGTGAGGGAGTGGTACCGCTTCCACTACATC AACATCCTGTCGAGGCTGCCAGAGACTCTGCCATCCCTGGAGGAGGACACGCTGGGCAACTTCATCTTCG CCTGCCGCTTCAACCAGGTCTCCTGCAACCAGGCGAATTACTCTCACTTCCACCACCCGATGTATGGAAA CTGCTATACTTTCAATGACAAGAACAACTCCAACCTCTGGATGTCTTCCATGCCTGGAATCAACAACGGT CTGTCCCTGATGCTGCGCGCAGAGCAGAATGACTTCATTCCCCTGCTGTCCACAGTGACTGGGGCCCGGG TAATGGTGCACGGGCAGGATGAACCTGCCTTTATGGATGATGGTGGCTTTAACTTGCGGCCTGGCGTGGA GACCTCCATCAGCATGAGGAAGGAAACCCTGGACAGACTTGGGGGCGATTATGGCGACTGCACCAAGAAT GGCAGTGATGTTCCTGTTGAGAACCTTTACCCTTCAAAGTACACACAGCAGGTGTGTATTCACTCCTGCT TCCAGGAGAGCATGATCAAGGAGTGTGGCTGTGCCTACATCTTCTATCCGCGGCCCCAGAACGTGGAGTA CTGTGACTACAGAAAGCACAGTTCCTGGGGGTACTGCTACTATAAGCTCCAGGTTGACTTCTCCTCAGAC CACCTGGGCTGTTTCACCAAGTGCCGGAAGCCATGCAGCGTGACCAGCTACCAGCTCTCTGCTGGTTACT CACGATGGCCCTCGGTGACATCCCAGGAATGGGTCTTCCAGATGCTATCGCGACAGAACAATTACACCGT CAACAACAAGAGAAATGGAGTGGCCAAAGTCAACATCTTCTTCAAGGAGCTGAACTACAAAACCAATTCT GAGTCTCCCTCTGTCACGATGGTCACCCTCCTGTCCAACCTGGGCAGCCAGTGGAGCCTGTGGTTCGGCT CCTCGGTGTTGTCTGTGGTGGAGATGGCTGAGCTCGTCTTTGACCTGCTGGTCATCATGTTCCTCATGCT GCTCCGAAGGTTCCGAAGCCGATACTGGTCTCCAGGCCGAGGGGGCAGGGGTGCTCAGGAGGTAGCCTCC ACCCTGGCATCCTCCCCTCCTTCCCACTTCTGCCCCCACCCCATGTCTCTGTCCTTGTCCCAGCCAGGCC CTGCTCCCTCTCCAGCCTTGACAGCCCCTCCCCCTGCCTATGCCACCCTGGGCCCCCGCCCATCTCCAGG GGGCTCTGCAGGGGCCAGTTCCTCCACCTGTCCTCTGGGGGGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228538 representing NM_001159575
Red=Cloning site Green=Tags(s) MSSIKGNKLEEQDPRPLQPIPGLMEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEA LIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLN INLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHP LQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYI NILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNG LSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKN GSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSD HLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNS ESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVAS TLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159575 |
ORF Size | 2076 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159575.1, NP_001153047.1 |
RefSeq ORF | 2079 bp |
Locus ID | 6337 |
Cytogenetics | 12p13.31 |
Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
Protein Pathways | Taste transduction |
MW | 78.1 kDa |
Gene Summary | 'Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC327173 | SCNN1A (untagged)-Human sodium channel nonvoltage-gated 1 alpha (SCNN1A) transcript variant 3 |
USD 1,410.00 |
|
RG228538 | SCNN1A (GFP-tagged) - Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 3 |
USD 850.00 |
|
RC228538L3 | Lenti-ORF clone of SCNN1A (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 3 |
USD 970.00 |
|
RC228538L4 | Lenti-ORF clone of SCNN1A (mGFP-tagged)-Human sodium channel, nonvoltage-gated 1 alpha (SCNN1A), transcript variant 3 |
USD 970.00 |
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