SLC15A2 (NM_001145998) Human Tagged ORF Clone

CAT#: RC228544

SLC15A2 (Myc-DDK-tagged)-Human solute carrier family 15 (H+/peptide transporter), member 2 (SLC15A2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC15A2
• Synonyms: PEPT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228544 representing NM_001145998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTTTCCAGAAAAATGAGTCCAAGGAAACTCTTTTTTCACCTGTCTCCATTGAAGAGGTACCAC
  CTCGACCACCTAGCCCTCCAAAGAAGCCATCTCCGACAATCTGTGGCTCCAACTATCCACTGAGCATTGC
  CTTCATTGTGGTGAATGAATTCTGCGAGCGCTTTTCCTATTATGGAATGAAAGCTGTGCTGATCCTGTAT
  TTCCTGTATTTCCTGCACTGGAATGAAGATACCTCCACATCTATATACCATGCCTTCAGCAGCCTCTGTT
  ATTTTACTCCCATCCTGGGAGCAGCCATTGCTGACTCGTGGTTGGGAAAATTCAAAGTCCTATCATTGAT
  CGGCCTGAGTCTAATAGCTTTGGGGACAGGAGGCATCAAACCCTGTGTGGCAGCTTTTGGTGGAGACCAG
  TTTGAAGAAAAACATGCAGAGGAACGGACTAGATACTTCTCAGTCTTCTACCTGTCCATCAATGCAGGGA
  GCTTGATTTCTACATTTATCACACCCATGCTGAGAGGAGATGTGCAATGTTTTGGAGAAGACTGCTATGC
  ATTGGCTTTTGGAGTTCCAGGACTGCTCATGGTAATTGCACTTGTTGTGTTTGCAATGGGAAGCAAAATA
  TACAATAAACCACCCCCTGAAGGAAACATAGTGGCTCAAGTTTTCAAATGTATCTGGTTTGCTATTTCCA
  ATCGTTTCAAGAACCGTTCTGGAGACATTCCAAAGCGACAGCACTGGCTAGACTGGGCGGCTGAGAAATA
  TCCAAAGCAGCTCATTATGGATGTAAAGGCACTGACCAGGGTACTATTCCTTTATATCCCATTGCCCATG
  TTCTGGGCTCTTTTGGATCAGCAGGGTTCACGATGGACTTTGCAAGCCATCAGGATGAATAGGAATTTGG
  GGTTTTTTGTGCTTCAGCCGGACCAGATGCAGGTTCTAAATCCCCTTCTGGTTCTTATCTTCATCCCGTT
  GTTTGACTTTGTCATTTATCGTCTGGTCTCCAAGTGTGGAATTAACTTCTCATCACTTAGGAAAATGGCT
  GTTGGTATGATCCTAGCATGCCTGGCATTTGCAGTTGCGGCAGCTGTAGAGATAAAAATAAATGAAATGG
  CCCCAGCCCAGCCAGGTCCCCAGGAGGTTTTCCTACAAGTCTTGAATCTGGCAGATGATGAGGTGAAGGT
  GACAGTGGTGGGAAATGAAAACAATTCTCTGTTGATAGAGTCCATCAAATCCTTTCAGAAAACACCACAC
  TATTCCAAACTGCACCTGAAAACAAAAAGCCAGGATTTTCACTTCCACCTGAAATATCACAATTTGTCTC
  TCTACACTGAGCATTCTGTGCAGGAGAAGAACTGGTACAGTCTTGTCATTCGTGAAGATGGGAACAGTAT
  CTCCAGCATGATGGTAAAGGATACAGAAAGCAGAACAACCAATGGGATGACAACCGTGAGGTTTGTTAAC
  ACTTTGCATAAAGATGTCAACATCTCCCTGAGTACAGATACCTCTCTCAATGTTGGTGAAGACTATGGTG
  TGTCTGCTTATAGAACTGTGCAAAGAGGAGAATACCCTGCAGTGCACTGTAGAACAGAAGATAAGAACTT
  TTCTCTGAATTTGGGTCTTCTAGACTTTGGTGCAGCATATCTGTTTGTTATTACTAATAACACCAATCAG
  GGTCTTCAGGCCTGGAAGATTGAAGACATTCCAGCCAACAAAATGTCCATTGCGTGGCAGCTACCACAAT
  ATGCCCTGGTTACAGCTGGGGAGGTCATGTTCTCTGTCACAGGTCTTGAGTTTTCTTATTCTCAGGCTCC
  CTCTAGCATGAAATCTGTGCTCCAGGCAGCTTGGCTATTGACAATTGCAGTTGGGAATATCATCGTGCTT
  GTTGTGGCACAGTTCAGTGGCCTGGTACAGTGGGCCGAATTCATTTTGTTTTCCTGCCTCCTGCTGGTGA
  TCTGCCTGATCTTCTCCATCATGGGCTACTACTATGTTCCTGTAAAGACAGAGGATATGCGGGGTCCAGC
  AGATAAGCACATTCCTCACATCCAGGGGAACATGATCAAACTAGAGACCAAGAAGACAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228544 representing NM_001145998
  Red=Cloning site Green=Tags(s)
  
  MNPFQKNESKETLFSPVSIEEVPPRPPSPPKKPSPTICGSNYPLSIAFIVVNEFCERFSYYGMKAVLILY
  FLYFLHWNEDTSTSIYHAFSSLCYFTPILGAAIADSWLGKFKVLSLIGLSLIALGTGGIKPCVAAFGGDQ
  FEEKHAEERTRYFSVFYLSINAGSLISTFITPMLRGDVQCFGEDCYALAFGVPGLLMVIALVVFAMGSKI
  YNKPPPEGNIVAQVFKCIWFAISNRFKNRSGDIPKRQHWLDWAAEKYPKQLIMDVKALTRVLFLYIPLPM
  FWALLDQQGSRWTLQAIRMNRNLGFFVLQPDQMQVLNPLLVLIFIPLFDFVIYRLVSKCGINFSSLRKMA
  VGMILACLAFAVAAAVEIKINEMAPAQPGPQEVFLQVLNLADDEVKVTVVGNENNSLLIESIKSFQKTPH
  YSKLHLKTKSQDFHFHLKYHNLSLYTEHSVQEKNWYSLVIREDGNSISSMMVKDTESRTTNGMTTVRFVN
  TLHKDVNISLSTDTSLNVGEDYGVSAYRTVQRGEYPAVHCRTEDKNFSLNLGLLDFGAAYLFVITNNTNQ
  GLQAWKIEDIPANKMSIAWQLPQYALVTAGEVMFSVTGLEFSYSQAPSSMKSVLQAAWLLTIAVGNIIVL
  VVAQFSGLVQWAEFILFSCLLLVICLIFSIMGYYYVPVKTEDMRGPADKHIPHIQGNMIKLETKKTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145998
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145998.1, NP_001139470.1
• RefSeq ORF: 2097 bp
• Locus ID: 6565
• Cytogenetics: 3q13.33
• Protein Families: Transmembrane
• MW: 78.3 kDa
• Gene Summary: 'The mammalian kidney expresses a proton-coupled peptide transporter that is responsible for the absorption of small peptides, as well as beta-lactam antibiotics and other peptide-like drugs, from the tubular filtrate. This transporter, SLC15A2, belongs to the same gene family as SLC15A1 (MIM 600544), the proton-coupled peptide transporter found in the small intestine (Liu et al, 1995 [PubMed 7756356]).[supplied by OMIM, Feb 2011]'