SLC12A4 (NM_001145962) Human Tagged ORF Clone

CAT#: RC228638

SLC12A4 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC12A4
• Synonyms: CTC-479C5.17; hKCC1; KCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228638 representing NM_001145962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGCGGGCGGGGCGTGTCGGCCCGGGGCGGCGGGGACAGCGGCGGGGACAGCGGCGGGCGGCTGGG
  ACGGCGGGTGCGGCGGGGCCGAGCCCGCACGATGCCTCACTTCACCGTGGTGCCAGTGGACGGGCCGAGG
  CGCGGCGACTATGACAACCTCGAGGGGCTCAGTTGGGTGGACTACGGGGAGCGCGCCGAGCTGGATGACT
  CGGACGGAAGAGCTGGACATCCGCCCAAAGGTATCGTCTCTTCTGGGAAAGCTCGTCAGCTACACCAACC
  TCACCCAGGGCGCCAAAGAGCATGAGGAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGC
  ACCCAGCATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTTATCCTCTTC
  CTGCGGCTGACCTGGATGGTGGGCACAGCAGGTGTGCTACAGGCCCTCCTCATCGTGCTTATCTGCTGCT
  GTTGTACCCTGCTGACGGCCATCTCCATGAGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGGGCTC
  CTATTTCATGATCTCTCGTTCACTGGGGCCAGAATTTGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGA
  ACAACATTCGCAGCAGCCATGTACATCCTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAG
  CTGCCATTTTTTACCCATCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAATATGCGTGTGTATGG
  GACCATTTTCCTGACCTTCATGACCCTGGTGGTGTTTGTGGGGGTCAAGTATGTGAACAAATTTGCCTCG
  CTCTTCCTGGCCTGTGTGATCATCTCCATCCTCTCCATCTATGCTGGGGGCATAAAGTCTATATTTGACC
  CTCCCGTGTTTCCGGTATGCATGCTGGGCAACAGGACCCTGTCCCGGGACCAGTTTGACATCTGTGCCAA
  GACAGCTGTAGTGGACAATGAGACAGTGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCCAACCTT
  ACGACCGACTCCTGTGACCCCTACTTCATGCTCAACAATGTGACCGAGATCCCTGGCATCCCCGGGGCAG
  CTGCTGGTGTGCTCCAGGAAAACCTGTGGAGCGCCTACCTGGAGAAGGGTGACATCGTGGAGAAGCATGG
  GCTGCCCTCCGCAGATGCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGCCACA
  TCCTTCACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTAACAGGCATCATGGCTGGCTCAAACCGCTCTG
  GGGACCTTCGTGACGCCCAGAAGTCTATCCCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGT
  GTACTTCAGCAGTGTGGTTCTCTTTGGTGCCTGCATTGAGGGTGTGGTTCTCCGGGACAAGTATGGCGAT
  GGTGTCAGCAGGAACTTGGTGGTGGGCACACTGGCCTGGCCTTCACCCTGGGTCATCGTCATCGGCTCCT
  TCTTTTCAACGTGTGGCGCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTGCCAA
  GGACAACATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTGAATGGTGAACCCACATGGGCACTC
  CTCCTGACGGCACTCATCGCCGAGCTGGGCATCCTCATCGCCTCCCTCGACATGGTGGCCCCCATCTTAT
  CCATGTTCTTTCTGATGTGCTACCTGTTCGTGAACCTCGCCTGTGCGGTGCAGACACTCCTGAGGACCCC
  CAACTGGCGGCCCCGGTTCAAGTACTATCACTGGGCGCTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCC
  CTTATGTTTGTCTCCTCCTGGTACTATGCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAATACA
  TCGAGTACCAAGGGGCTGAGAAGGAGTGGGGTGACGGGATCCGAGGCCTGTCCCTGAGCGCTGCCCGCTA
  CGCGCTGTTGCGGCTGGAGGAGGGGCCTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTGCTG
  AAGCTGGACGAGGACCTCCACGTGAAGTACCCGCGGCTCCTCACCTTCGCCTCCCAGCTCAAGGCTGGCA
  AGGGCCTGACCATTGTTGGTTCTGTCATCCAGGGGAGCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGC
  CGAGCAGACCATCAAGAACATGATGGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTGGCCAGC
  AAGGTGCGGGAGGGGCTGGCCCACCTCATCCAGTCCTGTGGCCTGGGAGGCATGCGGCATAACTCCGTGG
  TGCTGGGCTGGCCCTACGGCTGGCGACAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGT
  GCGCTGCACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATCGCCTTCTACCCCAGCAACCAC
  GAGCGCTACCTGGAGGGCCACATAGACGTGTGGTGGATCGTGCACGATGGTGGCATGCTCATGCTTCTGC
  CCTTCCTGCTGCGCCAGCATAAGGTCTGGAGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGATGGA
  TGACAACAGCATCCAGATGAAGAAGGACCTGGCTGTCTTTCTGTACCATCTGCGCCTTGAGGCCGAGGTG
  GAGGTGGTGGAGATGCATAACAGTGACATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGC
  GGTCGCAGATGCTGCGGCAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCCCAGCTGGTCAAGGA
  TCGGCACTCGGCCCTGCGGCTGGAGAGCCTGTACTCGGACGAGGAAGATGAGTCTGCAGTGGGGGCTGAC
  AAGATCCAGATGACGTGGACCAGGGACAAGTACATGACTGAGACCTGGGACCCCAGCCATGCCCCTGACA
  ATTTCCGGGAGCTGGTGCACATTAAGCCGGACCAATCCAATGTGCGGCGCATGCACACTGCTGTGAAGCT
  CAATGAAGTCATTGTCACGCGCTCCCACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCACCCAGG
  AACAGTGAGGGCGACGAGAACTACATGGAGTTCCTCGAGGTGCTGACCGAGGGCCTTGAGCGGGTGCTGT
  TGGTGCGCGGTGGTGGCCGTGAAGTCATCACCATCTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228638 representing NM_001145962
  Red=Cloning site Green=Tags(s)
  
  MRAGGACRPGAAGTAAGTAAGGWDGGCGGAEPARCLTSPWCQWTGRGAATMTTSRGSVGWTTGSAPSWMT
  RTEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILF
  LRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLG
  TTFAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFAS
  LFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTAVVDNETVATQLWSFFCHSPNL
  TTDSCDPYFMLNNVTEIPGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIAT
  SFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKYGD
  GVSRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKVNGEPTWAL
  LLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLA
  LMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLL
  KLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVAS
  KVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNH
  ERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRLEAEV
  EVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGAD
  KIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPR
  NSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145962
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145962.1, NP_001139434.1
• RefSeq ORF: 3264 bp
• Locus ID: 6560
• Cytogenetics: 16q22.1
• Protein Families: Transmembrane
• MW: 119.6 kDa
• Gene Summary: 'This gene encodes a member of the SLC12A transporter family. The encoded protein mediates the coupled movement of potassium and chloride ions across the plasma membrane. This gene is expressed ubiquitously. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2013]'