TJAP1 (NM_001146018) Human Tagged ORF Clone

CAT#: RC228954

TJAP1 (Myc-DDK-tagged)-Human tight junction associated protein 1 (peripheral) (TJAP1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TJAP1
• Synonyms: PILT; TJP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228954 representing NM_001146018
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAGTGCCGCCCCTGCTAAGAAACCCTACCGTAAGGCACCACCAGAGCATCGGGAGCTGCGTTTGG
  AAATTCCTGGATCCCGGCTTGAGCAGGAGGAACCCCTGACTGATGCAGAAAGGATGAAGCTCTTACAGGA
  GGAGAATGAAGAGCTTCGCCGGCGCCTGGCCTCCGCCACCAGACGCACTGAGGCCCTGGAACGTGAGCTG
  GAAATTGGGCAGGACTGCCTGGAGCTGGAGCTGGGCCAGAGCCGCGAGGAGCTGGACAAATTTAAGGATA
  AGTTCCGCAGGCTGCAGAACAGCTACACGGCTTCCCAGCGGACCAACCAGGAGTTGGAGGACAAGCTGCA
  CACACTGATCAAGAAGGCTGAGATGGATAGGAAGACGCTGGACTGGGAGATTGTGGAGCTGACCAACAAG
  CTGCTGGATGCCAAGAACACCATCAACAAGCTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACC
  TGGCTGTACAGCTCCTCAAGTGCAACAAGTCCCACTTCCGAAACCACAAGTTTGCCGATCTGCCCTGTGA
  GCTACAGGACATGGTTCGGAAACATTTGCACAGTGGTCAAGAGGCCGCCAGCCCAGGTCCTGCTCCCAGC
  CTAGCCCCAGGGGCTGTGGTGCCTACCTCAGTCATTGCCCGAGTGTTAGAGAAGCCGGAGTCTCTACTGC
  TCAATTCAGCCCAGTCAGGCAGCGCCGGGCGCCCCTTGGCTGAGGATGTCTTTGTGCATGTGGACATGAG
  TGAGGGTGTCCCAGGTGATCCAGCCAGTCCCCCGGCCCCTGGCAGCCCCACCCCACAACCCAATGGGGAG
  TGCCACTCTCTGGGTACTGCCAGGGGCTCCCCGGAGGAAGAGCTGCCCCTGCCAGCCTTTGAGAAGCTGA
  ACCCCTACCCAACCCCGTCTCCACCACACCCACTGTATCCTGGCCGCAGGGTAATAGAGTTCTCTGAGGA
  TAAGGTTCGGATCCCCCGCAACAGCCCCCTGCCCAACTGCACTTACGCTACCCGCCAGGCCATTTCCCTG
  AGCCTGGTAGAGGAGGGGAGTGAGCGGGCCCGCCCCAGCCCAGTGCCCAGCACCCCTGCCTCAGCCCAGG
  CCTCACCCCACCACCAGCCCAGCCCAGCACCCCTAACACTCAGTGCCCCAGCTAGCTCTGCCAGCTCTGA
  AGAGGACCTGCTGGTCAGCTGGCAGCGGGCATTTGTGGACCGTACTCCACCACCTGCTGCTGTGGCCCAG
  CGCACAGCCTTTGGACGCGATGCCCTCCCTGAGCTGCAGCGCCATTTTGCCCATAGCCCCGCTGACAGAG
  ATGAGGTGGTCCAGGCACCTTCTGCCCGACCCGAAGAGAGTGAGCTTTTGCTACCCACAGAACCTGACTC
  TGGCTTTCCCAGGGAGGAAGAAGAGCTGAACCTGCCTATCAGTCCTGAGGAAGAGCGCCAGAGCCTGCTG
  CCCATTAACAGGGGCACAGAGGAGGGGCCAGGCACTTCCCACACCGAGGGCAGGGCCTGGCCACTCCCCA
  GCTCCAGTCGCCCCCAGCGCAGCCCCAAGAGGATGGGGGTTCACCACCTGCACCGCAAGGACAGCCTGAC
  CCAGGCCCAGGAGCAGGGCAACCTGCTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228954 representing NM_001146018
  Red=Cloning site Green=Tags(s)
  
  MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALEREL
  EIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNK
  LLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPS
  LAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGE
  CHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISL
  SLVEEGSERARPSPVPSTPASAQASPHHQPSPAPLTLSAPASSASSEEDLLVSWQRAFVDRTPPPAAVAQ
  RTAFGRDALPELQRHFAHSPADRDEVVQAPSARPEESELLLPTEPDSGFPREEEELNLPISPEEERQSLL
  PINRGTEEGPGTSHTEGRAWPLPSSSRPQRSPKRMGVHHLHRKDSLTQAQEQGNLLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146018
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146018.1, NP_001139490.1
• RefSeq ORF: 1644 bp
• Locus ID: 93643
• Protein Pathways: Tight junction
• MW: 60.5 kDa
• Gene Summary: This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome. [provided by RefSeq, Mar 2009]