ME3 (NM_001161586) Human Tagged ORF Clone

CAT#: RC228973

  • TrueORF®

ME3 (Myc-DDK-tagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 3


  "NM_001161586" in other vectors (4)

Reconstitution Protocol

USD 420.00

4 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ME3
Synonyms NADP-ME
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC228973 representing NM_001161586
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCGCCCTCCCGC
GCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGGCGCGCCCTGTGCCCCTGAA
GAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACAAGGGGATGGCCTTTACCCTTGAAGAAAGG
CTGCAGCTTGGAATCCACGGCCTAATCCCGCCCTGCTTTCTGGGCCAGGACGTCCAGCTCCTCCGAATCA
TGGGATATTACGAGCGGCAGCAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAA
CGAGAAGCTCTTCTACCGAGTGCTGACTTCGGATGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCACCATTCATG
ACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATTAAGGCCGTGGTGGTGACTGA
TGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTACGGCATGGGCATCCCTGTGGGCAAGCTGGCC
CTGTACACGGCATGCGGAGGGGTGAACCCGCAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACA
ATGAGGAGCTGCTCAGAGACCCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGA
TGACTTGCTGGATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATGTTCAATGATG
ACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTGCGAATCACCAACAACAAGCT
TTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCAGCTATGGGCATTGCCCACCTCCTTGTCATG
GCCCTAGAGAAAGAAGGTGTACCGAAGGCAGAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGC
TCATTGTCAAGGGGAGGAGCCACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAA
CTCCCTGGAGGAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCCCTGAGCAACC
CCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAGGGCCGAGGGATTTTTGCCAG
TGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAGACCTTCATTCCTGGGCAGGGAAACAATGCT
TACGTGTTCCCCGGGGTGGCACTGGGAGTCATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCC
TCCTGACAGCAGAGCAAATTGCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACC
ACTCAGCACCATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTACACTCCAGACT
ATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAATGTTCAGACGGTC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC228973 representing NM_001161586
Red=Cloning site Green=Tags(s)

MGAALGTGTRLAPWPGRACGALPRWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEER
LQLGIHGLIPPCFLGQDVQLLRIMGYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPT
VGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLA
LYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFE
DFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITNNKLSNHVFVFQGAGEAAMGIAHLLVM
ALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGA
FTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNA
YVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHN
LASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001161586
ORF Size 1812 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001161586.1, NP_001155058.1
RefSeq ORF 1815
Locus ID 10873
Protein Pathways Metabolic pathways, Pyruvate metabolism
MW 66.9 kDa
Gene Summary Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

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