DBC2 (RHOBTB2) (NM_001160036) Human Tagged ORF Clone

CAT#: RC229009

RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RHOBTB2
• Synonyms: DBC2; EIEE64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229009 representing NM_001160036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGCCTGGAGAAAAGGCCCCGATGGCCCCCAGAAGACCTCTTCAGACAGCATGTCCCGTTTAATGG
  ATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTGGGGGACAACGCCGT
  GGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTCACCCAGTACCAGCTGCTGGCCACG
  CATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGCCAGGAGGTGCTGGAACGCTCCCGAGACG
  TGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGTCGCTT
  TGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCACCATGTC
  AAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGCTGCCAGT
  TGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCTAGGCCCATCAAACC
  TAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAGCTGGGCATCCCCTACTATGAGACC
  AGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTTGACAACGCCATCCGAGCTGCACTCATCTCCCGCC
  GCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCACCCTTCCTACC
  CCCCAAGCCACCGCCCCCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAGTGCCCCGCCCAC
  CTCCTGGAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGCATCTTTGCCCACA
  AGATCTACCTCTCCACCTCTTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTGAGTGAGGGGGAGCT
  GGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAGGGCCACTCTGATCAACACCACCAC
  CATCACCACCACCACCATGGGCGAGACTTCCTGCTCCGAGCAGCCAGCTTTGACGTGTGCGAGAGCGTGG
  ATGAGGCTGGGGGCTCCGGTCCTGCTGGCCTCCGTGCTTCCACCAGCGACGGGATCTTACGGGGCAACGG
  AACAGGGTACCTACCGGGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCTTTTGTGAGCATCCAGGAA
  GAGATGGCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTGAAGATGGACAGTTCCATCC
  AGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTAGATGAGAACGAGCGTGACCT
  CATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTGCGCATGATGGTGGCCAACATTCTC
  AACAATGAGGCCTTCATGAACCAGGAGATCACCAAGGCCTTCCACGTCCGCCGGACCAACCGGGTTAAGG
  AGTGCTTGGCAAAAGGCACCTTCTCAGATGTGACCTTCATCCTGGATGATGGCACCATCAGCGCCCACAA
  GCCCCTGTTGATTTCCAGCTGTGACTGGATGGCTGCCATGTTTGGGGGGCCATTTGTGGAGAGCTCCACC
  CGGGAGGTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCCGTGCTGGAATACCTCTACACCGGCA
  TGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATTCTAGCCAACCGCCTCTGCCTGCCACA
  CCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATGGAAGCGACCCAGATGATGGTGGACATC
  GATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAGTTCCACTGTGCGTACCAGCTGGCCGACTGGTGTC
  TCCACCACATCTGCACCAACTACAACAACGTGTGCCGCAAGTTCCCCCGAGACATGAAGGCCATGTCCCC
  AGAAAACCAGGAGTATTTCGAGAAGCATCGGTGGCCACCTGTGTGGTACCTGAAGGAGGAAGATCATTAC
  CAGCGGGCACGGAAGGAGCGTGAGAAGGAGGACTACCTCCACCTCAAGCGGCAGCCCAAACGGCGTTGGC
  TCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCAGCCTCCTCCTCATCCCCATCTTCCTCCTCGGC
  TGTGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229009 representing NM_001160036
  Red=Cloning site Green=Tags(s)
  
  MQAWRKGPDGPQKTSSDSMSRLMDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLAT
  HVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHV
  KTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYET
  SVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAH
  LLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHH
  HHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQE
  EMAEDPLTYKSRLMVVVKMDSSIQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANIL
  NNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESST
  REVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDI
  DGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHY
  QRARKEREKEDYLHLKRQPKRRWLFWNSPSSPSSSAASSSSPSSSSAVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160036
• ORF Size: 2247 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001160036.1, NP_001153508.1
• RefSeq Size: 5261
• RefSeq ORF: 2250
• Locus ID: 23221
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 85.1 kDa
• Gene Summary: The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]