KDM3A / JHDM2A (KDM3A) (NM_001146688) Human Tagged ORF Clone

CAT#: RC229072

KDM3A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 3A (KDM3A), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY432099) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229072 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KDM3A protein (Cat# TP329072). The protein was produced from HEK293T cells transfected with KDM3A cDNA clone (Cat# RC229072) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KDM3A
• Synonyms: JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229072 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCACGCTCGGAGAAAGTTGGCCGGTATTGGTGGGGAGGAGGTTTCTCAGTCTGTCCGCAGCCG
  ACGGCAGCGATGGCAGCCACGACAGCTGGGACGTGGAGCGCGTCGCCGAGTGGCCCTGGCTCTCCGGGAC
  CATTCGAGCTGTTTCCCACACCGACGTTACCAAGAAGGATCTGAAGGTGTGTGTGGAATTTGATGGGGAA
  TCTTGGAGGAAAAGAAGATGGATAGAAGTCTACAGCCTTCTAAGGAGAGCATTTTTAGTAGAACATAATT
  TGGTTTTAGCTGAACGAAAGTCACCTGAAATTTCTGAACGAATTGTACAGTGGCCTGCAATAACGTACAA
  ACCTCTGTTGGACAAAGCTGGTTTGGGATCCATAACTTCTGTTCGCTTTCTGGGAGATCAACAAAGAGTA
  TTTCTTTCTAAAGACCTTTTGAAGCCTATACAGGATGTAAACAGTCTTCGACTTTCTCTTACGGATAATC
  AGATTGTCAGTAAAGAATTTCAAGCTTTGATTGTGAAGCATTTAGATGAAAGCCATCTTTTAAAAGGTGA
  CAAAAACTTAGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACC
  GTTATAAATGGAAACCCAGCATCAAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG
  TTGATCCGTCACTGATTCATGTTGAAGTTGTACACGATAACCTTGTGACATGTGGTAATTCTGCAAGAAT
  TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAATGGAACCCTGGTTTCCAAACAAGCAAAATCTTGCTCT
  GAGGCCTCTCCCAGTATGTGTCCTGTGCAGTCTGTACCTACAACAGTTTTTAAGGAGATACTGCTTGGCT
  GTACTGCGGCAACTCCACCTAGTAAGGACCCAAGACAGCAAAGTACTCCCCAGGCTGCCAACTCTCCACC
  TAACCTTGGAGCAAAAATTCCTCAAGGATGTCATAAACAAAGTTTACCAGAGGAAATTTCTTCCTGTCTA
  AATACAAAGTCTGAAGCTCTGAGAACAAAACCAGATGTCTGCAAAGCAGGGTTGCTCTCAAAGTCCTCTC
  AGATTGGAACTGGAGACTTGAAAATTCTGACTGAGCCAAAAGGCAGCTGTACTCAGCCTAAGACAAACAC
  TGATCAGGAAAACAGATTGGAGTCTGTTCCACAAGCATTGACTGGCCTTCCTAAGGAGTGCTTACCTACA
  AAGGCTTCTTCTAAGGCAGAATTGGAAATTGCCAATCCTCCTGAACTGCAGAAGCACCTAGAACATGCAC
  CTTCCCCATCGGATGTTTCAAATGCACCAGAAGTGAAAGCAGGTGTCAATAGTGATAGCCCTAATAACTG
  TTCAGGAAAAAAGGTAGAACCTTCAGCTTTAGCTTGCCGATCACAGAATTTAAAGGAATCTTCAGTAAAA
  GTAGATAATGAAAGCTGTTGTTCAAGAAGCAACAATAAAATCCAGAATGCCCCATCCAGGAAGTCGGTTT
  TGACAGACCCAGCTAAACTCAAAAAGCTGCAACAGAGTGGCGAGGCCTTCGTACAGGATGATTCTTGTGT
  GAACATCGTGGCACAGTTGCCTAAATGCCGAGAGTGTCGCTTGGACAGTCTCCGCAAGGATAAGGAGCAA
  CAGAAGGACTCACCTGTGTTTTGCCGCTTCTTTCACTTCAGGAGGTTACAATTCAACAAACATGGTGTGT
  TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAATGAAGCAATTGGCTTGTGGTTACCTTTAAC
  CAAAAACGTTGTGGGGATTGATTTGGACACAGCAAAGTACATCTTGGCCAACATTGGAGACCACTTCTGT
  CAAATGGTGATTTCTGAAAAGGAAGCTATGTCAACTATTGAGCCACACAGACAGGTTGCTTGGAAGCGAG
  CTGTCAAAGGTGTTCGAGAAATGTGTGATGTGTGCGACACCACCATCTTCAACCTGCACTGGGTGTGTCC
  TCGGTGTGGGTTTGGAGTATGTGTGGACTGCTACCGGATGAAGAGAAAGAATTGCCAACAGGGTGCTGCT
  TACAAGACTTTCTCTTGGCTAAAATGTGTGAAGAGTCAGATACATGAACCAGAGAACTTAATGCCCACAC
  AGATCATTCCTGGAAAAGCACTCTATGATGTTGGAGACATTGTTCATTCTGTAAGAGCGAAATGGGGAAT
  AAAGGCAAACTGCCCTTGTTCAAACAGGCAATTCAAACTCTTTTCAAAGCCAGCCTCAAAGGAAGACCTA
  AAACAGACTTCTTTAGCTGGAGAAAAACCGACTCTTGGTGCAGTGCTCCAGCAGAATCCCTCAGTGTTGG
  AGCCAGCAGCTGTGGGTGGGGAAGCAGCCTCCAAGCCAGCCGGCAGCATGAAGCCTGCCTGTCCAGCCAG
  CACATCTCCTCTAAACTGGCTGGCCGACCTAACCAGCGGGAATGTCAACAAGGAAAACAAGGAAAAACAA
  CCAACAATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCCTCCCACCTTTAAGCAAATCCAGCA
  CAGTCCTCCATACGTTTAACAGCACAATTTTGACACCCGTAAGCAACAACAATTCTGGTTTCCTCCGGAA
  TCTCTTGAATTCTTCTACAGGAAAGACAGAAAATGGACTCAAGAATACACCAAAAATCCTTGATGACATC
  TTTGCCTCTTTGGTGCAAAATAAGACGACTTCTGATTTATCTAAGAGGCCTCAAGGACTAACCATCAAGC
  CCAGCATTCTGGGCTTTGACACTCCTCACTATTGGCTTTGTGATAATCGCTTGCTGTGCTTGCAAGACCC
  CAACAATAAGAGCAACTGGAATGTGTTTAGGGAGTGCTGGAAACAAGGGCAGCCAGTGATGGTGTCTGGA
  GTGCATCATAAATTGAACTCTGAACTTTGGAAACCTGAATCCTTCAGGAAAGAGTTTGGTGAGCAGGAAG
  TAGACCTAGTTAATTGTAGGACCAATGAAATCATCACAGGAGCCACAGTAGGAGACTTCTGGGATGGATT
  TGAAGATGTTCCAAATCGTTTGAAAAATGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGGCCACCA
  GGAGAAGATTTTAGAGATATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGCCCGAGT
  ACACAAGGCGAGATGGCAAACTGAATTTGGCCTCTAGGCTGCCAAACTACTTTGTTCGGCCAGATCTGGG
  CCCCAAGATGTATAATGCTTATGGATTAATCACTCCTGAAGATCGGAAATATGGAACAACAAATCTTCAC
  TTAGATGTATCTGATGCAGCTAATGTCATGGTCTATGTGGGAATTCCCAAAGGACAGTGTGAGCAAGAAG
  AAGAAGTCCTTAAGACCATCCAAGATGGAGATTCTGACGAACTCACAATAAAGCGATTTATTGAAGGAAA
  AGAGAAGCCAGGAGCACTGTGGCACATATATGCTGCAAAGGACACGGAGAAGATAAGGGAATTTCTTAAA
  AAGGTATCAGAAGAGCAAGGTCAAGAAAACCCAGCAGACCACGATCCTATTCATGATCAAAGCTGGTATT
  TAGACCGATCATTAAGAAAACGTCTTCATCAAGAGTATGGAGTTCAAGGCTGGGCTATTGTACAGTTTCT
  TGGGGATGTGGTGTTTATCCCGGCAGGAGCTCCACATCAGGTTCATAACTTATATAGCTGCATCAAAGTG
  GCTGAAGATTTTGTTTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGATATCTGT
  CACAGACTCATACCAATCACGAAGATAAATTACAGGTGAAGAATGTTATCTACCATGCAGTGAAAGATGC
  AGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTTGGCAAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229072 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAVSHTDVTKKDLKVCVEFDGE
  SWRKRRWIEVYSLLRRAFLVEHNLVLAERKSPEISERIVQWPAITYKPLLDKAGLGSITSVRFLGDQQRV
  FLSKDLLKPIQDVNSLRLSLTDNQIVSKEFQALIVKHLDESHLLKGDKNLVGSEVKIYSLDPSTQWFSAT
  VINGNPASKTLQVNCEEIPALKIVDPSLIHVEVVHDNLVTCGNSARIGAVKRKSSENNGTLVSKQAKSCS
  EASPSMCPVQSVPTTVFKEILLGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCL
  NTKSEALRTKPDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQENRLESVPQALTGLPKECLPT
  KASSKAELEIANPPELQKHLEHAPSPSDVSNAPEVKAGVNSDSPNNCSGKKVEPSALACRSQNLKESSVK
  VDNESCCSRSNNKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIVAQLPKCRECRLDSLRKDKEQ
  QKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAIGLWLPLTKNVVGIDLDTAKYILANIGDHFC
  QMVISEKEAMSTIEPHRQVAWKRAVKGVREMCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAA
  YKTFSWLKCVKSQIHEPENLMPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPASKEDL
  KQTSLAGEKPTLGAVLQQNPSVLEPAAVGGEAASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQ
  PTMPILKNEIKCLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGLKNTPKILDDI
  FASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNKSNWNVFRECWKQGQPVMVSG
  VHHKLNSELWKPESFRKEFGEQEVDLVNCRTNEIITGATVGDFWDGFEDVPNRLKNEKEPMVLKLKDWPP
  GEDFRDMMPSRFDDLMANIPLPEYTRRDGKLNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLH
  LDVSDAANVMVYVGIPKGQCEQEEEVLKTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREFLK
  KVSEEQGQENPADHDPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCIKV
  AEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSFGKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146688
• ORF Size: 3963 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146688.1, NP_001140160.1
• RefSeq Size: 4638 bp
• RefSeq ORF: 3966 bp
• Locus ID: 55818
• MW: 147.3 kDa
• Gene Summary: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]