NTE (PNPLA6) (NM_001166113) Human Tagged ORF Clone

CAT#: RC229073

PNPLA6 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 6 (PNPLA6), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNPLA6
• Synonyms: BNHS; iPLA2delta; LNMS; NTE; NTEMND; OMCS; SPG39; sws
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229073 representing NM_001166113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTCCGCTGCAAACTGGAATGGTGCTTGGCGTGATGATCGGGGCCGGAGTGGCGGTGGTGGTCA
  CGGCCGTGCTCATCCTCCTGGTGGTGCGGAGGCTGCGAGTGCCAAAAACCCCAGCCCCGGATGGCCCCCG
  GTATCGGTTCCGGAAGAGGGACAAAGTGCTCTTCTATGGCCGGAAGATTATGCGGAAGGTGTCACAATCC
  ACCTCCTCCCTCGTGGATACCTCTGTCTCCGCCACCTCCCGGCCACGCATGAGGAAGAAACTGAAGATGC
  TCAACATTGCCAAGAAGATCCTGCGCATCCAGAAAGAGACGCCCACGCTGCAGCGGAAGGAGCCCCCGCC
  CGCAGTGCTAGAAGCTGACCTGACCGAGGGCGACCTGGCTAACTCCCATCTGCCCTCTGAAGTGCTTTAT
  ATGCTCAAGAACGTCCGGGTGCTGGGCCACTTCGAGAAGCCACTCTTCCTGGAGCTCTGCCGCCACATGG
  TCTTCCAGCGGCTGGGCCAGGGTGACTACGTCTTCCGGCCGGGCCAGCCAGATGCCAGCATCTACGTGGT
  GCAGGACGGGCTGCTGGAGCTCTGTCTGCCAGGGCCTGACGGGAAGGAGTGTGTGGTGAAGGAAGTGGTT
  CCTGGGGACAGCGTCAACAGCCTTCTCAGCATCCTGGATGTCATCACCGGTCACCAGCATCCCCAGCGGA
  CCGTGTCTGCCCGGGCGGCCCGGGACTCCACGGTGCTGCGCCTGCCGGTGGAAGCATTCTCCGCGGTCTT
  CACCAAGTACCCGGAGAGCTTGGTGCGGGTCGTGCAGATCATCATGGTGCGGCTGCAGCGAGTCACCTTC
  CTGGCACTGCACAACTACCTGGGTCTGACCAATGAGCTCTTCAGCCACGAGATCCAGCCCCTGCGTCTGT
  TCCCCAGCCCCGGCCTCCCAACTCGCACCAGCCCTGTGCGGGGCTCCAAGAGAATGGTCAGCACCTCAGC
  TACAGACGAGCCCAGGGAGACCCCAGGGCGGCCACCCGATCCCACCGGGGCCCCGCTGCCTGGACCTACA
  GGGGACCCTGTGAAGCCCACATCCCTGGAAACCCCCTCGCCCCCTCTGCTGAGCCGCTGCGTCTCCATGC
  CAGGGGACATCTCAGGCTTGCAGGGTGGCCCCCGCTCCGACTTCGACATGGCCTATGAGCGTGGCCGGAT
  CTCCGTGTCCCTGCAAGAAGAGGCCTCCGGGGGGTCCCTGGCAGCCCCCGCTCGGACCCCCACTCAGGAG
  CCTCGTGAGCAGCCGGCAGGCGCCTGTGAATACAGCTACTGTGAGGATGAGTCGGCCACTGGTGGCTGCC
  CTTTCGGGCCCTACCAGGGCCGCCAGACCAGCAGCATCTTCGAGGCAGCAAAGCAGGAGCTGGCCAAGCT
  GATGCGGATTGAGGACCCCTCCCTCCTGAACAGCAGAGTCTTGCTGCACCACGCCAAAGCTGGCACCATC
  ATTGCCCGCCAGGGAGACCAGGACGTGAGCCTGCACTTCGTGCTCTGGGGCTGCCTGCACGTGTACCAGC
  GCATGATCGACAAGGCGGAGGACGTGTGCCTGTTCGTAGCGCAGCCCGGGGAACTGGTGGGGCAGCTGGC
  GGTGCTCACTGGCGAACCTCTCATCTTCACACTGCGAGCCCAACGCGACTGCACCTTCCTGCGGATCTCC
  AAGTCCGACTTCTATGAGATCATGCGCGCACAGCCCAGTGTGGTGCTGAGTGCGGCGCACACGGTGGCAG
  CCAGGATGTCGCCCTTCGTGCGCCAGATGGACTTCGCCATCGACTGGACTGCAGTGGAGGCGGGACGCGC
  GCTGTACAGGCAGGGCGACCGCTCCGACTGCACTTACATCGTGCTCAATGGGCGGCTGCGTAGCGTGATC
  CAGCGAGGCAGTGGCAAGAAGGAGCTGGTGGGCGAGTACGGCCGCGGCGACCTCATCGGCGTGGTGGAGG
  CACTGACCCGGCAGCCGCGAGCCACGACGGTGCACGCGGTGCGCGACACGGAGCTGGCCAAGCTTCCCGA
  GGGCACCTTGGGTCACATCAAACGCCGGTACCCGCAGGTCGTGACCCGCCTTATCCACCTACTGAGCCAG
  AAAATTCTAGGGAATTTGCAGCAGCTGCAAGGACCCTTCCCAGCAGGCTCTGGGTTGGGTGTGCCCCCAC
  ACTCGGAACTCACCAACCCAGCCAGCAACCTGGCAACTGTGGCAATCCTGCCTGTGTGTGCTGAGGTCCC
  CATGGTGGCCTTCACGCTGGAGCTGCAGCACGCCCTGCAGGCCATCGGTCCGACGCTACTCCTTAACAGT
  GACATCATCCGGGCACGCCTGGGGGCCTCCGCACTGGATAGCATCCAAGAGTTCCGGCTGTCAGGGTGGC
  TGGCCCAGCAGGAGGATGCACACCGTATCGTACTCTACCAGACGGACGCCTCGCTGACGCCCTGGACCGT
  GCGCTGCCTGCGACAGGCCGACTGCATCCTCATTGTGGGCCTGGGGGACCAGGAGCCTACCCTCGGCCAG
  CTGGAGCAGATGCTGGAGAACACGGCTGTGCGCGCCCTTAAGCAGCTAGTCCTGCTCCACCGAGAGGAGG
  GCGCGGGCCCCACGCGCACCGTGGAGTGGCTAAATATGCGCAGCTGGTGCTCGGGGCACCTGCACCTGCG
  CTGTCCGCGCCGCCTCTTTTCGCGCCGCAGCCCTGCCAAGCTGCATGAGCTCTACGAGAAGGTTTTCTCC
  AGGCGCGCGGACCGGCACAGCGACTTCTCCCGCTTGGCGAGGGTGCTCACGGGGAACACCATTGCCCTTG
  TGCTAGGCGGGGGCGGGGCCAGGGGCTGCTCGCACATCGGAGTACTAAAGGCATTAGAGGAGGCGGGGGT
  CCCCGTGGACCTGGTGGGCGGCACGTCCATTGGCTCTTTCATCGGAGCGTTGTACGCGGAGGAGCGCAGC
  GCCAGCCGCACGAGGCAGCGGGCCCGGGAGTGGGCCAAGAGCATGACTTCGGTGCTGGAACCTGTGTTGG
  ACCTCACGTACCCAGTCACCTCCATGTTCACTGGGTCTGCCTTTAACCGCAGCATCCATCGGGTCTTCCA
  GGATAAGCAGATTGAGGACCTGTGGCTGCCTTACTTCAACGTGACCACAGATATCACCGCCTCAGCCATG
  CGAGTCCACAAAGATGGCTCCCTGTGGCGGTACGTGCGCGCCAGCATGACGCTGTCGGGCTACCTGCCCC
  CGCTGTGCGACCCCAAGGACGGGCACCTACTCATGGATGGCGGCTACATCAACAATCTGCCAGCGGACAT
  CGCCCGCAGCATGGGTGCCAAAACGGTCATCGCCATTGACGTGGGGAGCCAGGATGAGACGGACCTCAGC
  ACCTACGGGGACAGCCTGTCCGGCTGGTGGCTGCTGTGGAAGCGGCTGAATCCCTGGGCTGACAAGGTAA
  AGGTTCCAGACATGGCTGAAATCCAGTCCCGCCTGGCCTACGTGTCCTGTGTGCGGCAGCTAGAGGTTGT
  CAAGTCCAGCTCCTACTGCGAGTACCTGCGCCCGCCCATCGACTGCTTCAAGACCATGGACTTTGGGAAG
  TTCGACCAGATCTATGATGTGGGCTACCAGTACGGGAAGGCGGTGTTTGGAGGCTGGAGCCGTGGCAACG
  TCATTGAGAAAATGCTCACAGACCGGCGGTCTACAGACCTTAATGAGAGCCGCCGTGCAGACGTGCTTGC
  CTTCCCAAGCTCTGGCTTCACTGACTTGGCAGAGATTGTGTCCCGGATTGAGCCCCCCACGAGCTATGTC
  TCTGATGGCTGTGCTGACGGAGAGGAGTCAGATTGTCTGACAGAGTATGAGGAGGACGCCGGACCCGACT
  GCTCGAGGGATGAAGGGGGGTCCCCCGAGGGCGCAAGCCCCAGCACTGCCTCCGAGATGGAGGAGGAGAA
  GTCGATTCTCCGGCAACGACGCTGTCTGCCCCAGGAGCCGCCCGGCTCAGCCACAGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229073 representing NM_001166113
  Red=Cloning site Green=Tags(s)
  
  MEAPLQTGMVLGVMIGAGVAVVVTAVLILLVVRRLRVPKTPAPDGPRYRFRKRDKVLFYGRKIMRKVSQS
  TSSLVDTSVSATSRPRMRKKLKMLNIAKKILRIQKETPTLQRKEPPPAVLEADLTEGDLANSHLPSEVLY
  MLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVV
  PGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTF
  LALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRMVSTSATDEPRETPGRPPDPTGAPLPGPT
  GDPVKPTSLETPSPPLLSRCVSMPGDISGLQGGPRSDFDMAYERGRISVSLQEEASGGSLAAPARTPTQE
  PREQPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKQELAKLMRIEDPSLLNSRVLLHHAKAGTI
  IARQGDQDVSLHFVLWGCLHVYQRMIDKAEDVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRIS
  KSDFYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVI
  QRGSGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQ
  KILGNLQQLQGPFPAGSGLGVPPHSELTNPASNLATVAILPVCAEVPMVAFTLELQHALQAIGPTLLLNS
  DIIRARLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDASLTPWTVRCLRQADCILIVGLGDQEPTLGQ
  LEQMLENTAVRALKQLVLLHREEGAGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFS
  RRADRHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERS
  ASRTRQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAM
  RVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLS
  TYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGK
  FDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYV
  SDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166113
• ORF Size: 3981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166113.1, NP_001159585.1
• RefSeq ORF: 3984
• Locus ID: 10908
• Protein Families: Transmembrane
• MW: 146 kDa
• Gene Summary: This gene encodes a phospholipase that deacetylates intracellular phosphatidylcholine to produce glycerophosphocholine. It is thought to function in neurite outgrowth and process elongation during neuronal differentiation. The protein is anchored to the cytoplasmic face of the endoplasmic reticulum in both neurons and non-neuronal cells. Mutations in this gene result in autosomal recessive spastic paraplegia, and the protein is the target for neurodegeneration induced by organophosphorus compounds and chemical warfare agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]