MIER1 (NM_001077703) Human Tagged ORF Clone

CAT#: RC229242

MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MIER1
• Synonyms: ER1; MI-ER1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229242 representing NM_001077703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGGGCTTCTTCAGGCGGTGGCGGCAGCAGCGAAGGTGGCGGCGGCAGCAGCGGCAGCGGCTATG
  GTGTGGTCGCTCGATTCTCCCAGTGCCTGGCTGAGTTTCGGACGTGGTTAAGAACCAACTGGTTGAGGTT
  CAATGCAGACAAGACGGATGTGATGCTGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCA
  GATGACCATGAATTTGATCCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAG
  AGGAAGAAATGATGGAAGGAGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACAT
  GCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAA
  GAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGCTGTAGTG
  GGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGA
  TCCATCACAATCTGTTGCTTCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGAT
  ACAAATAGTGAAGTAGAAGAAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGG
  AGATTATGGTGGGCTCCATGTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAA
  AGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTT
  CTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGATCTCACATAA
  AAGACAATGAACAGGCTTTATATGAATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATT
  AAGATTTAATGTAAAAGCAGCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAA
  CAAGGGCTGAAGGCCTATGGAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTG
  GTGAATGTGTAGCATTCTATTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACG
  ATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGT
  GAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAACAGCCAGTCTG
  AGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGCATACTCCA
  AATGCTTCTTCCAGTTCATTTTTCAGCCATCAGTTCAAGAGCCAATGCCTTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229242 representing NM_001077703
  Red=Cloning site Green=Tags(s)
  
  MDGASSGGGGSSEGGGGSSGSGYGVVARFSQCLAEFRTWLRTNWLRFNADKTDVMLPSVESSSPGGSATS
  DDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELLSLYGYGSTVRLPEEDEE
  EEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFD
  TNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGICRYKENEKVYENDDQLLWDPEYLPEDKVIIF
  LKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKAAREELSVWTEEECRNFE
  QGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDES
  ESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGILQMLLPVHFSAISSRANAFLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077703
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077703.2, NP_001071171.2
• RefSeq ORF: 1461
• Locus ID: 57708
• MW: 54.4 kDa
• Gene Summary: This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013]