ZFP57 (NM_001109809) Human Tagged ORF Clone

CAT#: RC229273

ZFP57 (Myc-DDK-tagged)-Human zinc finger protein 57 homolog (mouse) (ZFP57)

Product Images

Western blot validation of overexpression lysate (Cat# LY432292) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229273 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFP57
• Synonyms: bA145L22; bA145L22.2; C6orf40; TNDM1; ZNF698
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229273 representing NM_001109809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGAACAGCTGAAGCCAATCGAACCTGTACAGAAGACGCTCCCATGGGTGGGCGAGGTAGCTGCCA
  CCCTGCAGGAAGCCATGAAGAGAGATTGCTGGAGGGAGGCACGGGTGAAGAAGAAGCCAGTCACCTTTGA
  GGATGTGGCAGTGAATTTCACCCAGGAAGAGTGGGACTGTCTAGATGCCAGCCAGAGGGTCCTTTACCAG
  GATGTTATGTCGGAAACCTTTAAGAATCTAACATCTGTGGCCAGAATCTTTCTGCATAAGCCAGAGCTAA
  TCACCAAGCTTGAGCAAGAAGAGGAACAGTGGAGAGAGTTTGTCCATCTCCCAAACACAGAAGGCCTTTC
  AGAAGGCAAGAAGAAAGAGCTTCGAGAACAACATCCCAGTCTGAGAGATGAGGGGACTAGTGATGACAAG
  GTCTTCCTTGCATGCAGAGGGGCCGGCCAGTGCCCCCTATCTGCCCCAGCTGGGACTATGGACAGGACCC
  GGGTGCTTCAAGCATCCCAGGCTGGGCCACCCTTTTTTTGCTACACCTGTGGCAAATGTTTCAGCAGGCG
  CTCCTACCTCTATAGCCACCAGTTTGTTCACAATCCCAAGCTGACTAACAGCTGCAGTCAGTGTGGGAAG
  TTGTTTCGGAGCCCCAAGTCCCTCAGCTATCACAGACGCATGCATCTTGGGGAGAGGCCCTTCTGTTGCA
  CGCTCTGTGACAAGACCTACTGTGATGCTTCTGGACTAAGTCGTCACCGCCGCGTCCATCTGGGTTACCG
  GCCCCATTCATGCTCTGTGTGTGGGAAGAGCTTCCGGGACCAGTCTGAGCTCAAACGCCACCAGAAGATA
  CACCAAAACCAGGAGCCAGTGGATGGAAACCAGGAGTGTACTTTGAGGATTCCAGGCACCCAGGCTGAAT
  TCCAGACACCCATCGCCAGAAGCCAGAGGTCCATCCAGGGGCTTTTGGATGTGAACCATGCACCAGTGGC
  CAGGTCCCAGGAACCCATATTTAGAACTGAGGGTCCTATGGCCCAGAACCAGGCATCTGTACTTAAGAAC
  CAAGCACCTGTGACCAGGACCCAGGCACCCATCACTGGAACCCTCTGTCAGGATGCCAGATCCAACTCTC
  ATCCAGTGAAGCCCTCAAGACTCAATGTCTTCTGTTGCCCCCATTGTTCTTTGACTTTTAGCAAGAAATC
  CTATCTCTCCAGACACCAGAAGGCCCACCTCACAGAGCCGCCCAACTACTGCTTCCATTGCAGCAAGTCT
  TTCAGCTCATTTTCCAGGCTGGTCAGACACCAGCAGACCCACTGGAAGCAGAAGAGCTACCTTTGCCCTA
  TCTGTGACCTCTCCTTTGGGGAGAAAGAGGGCCTTATGGATCACTGGAGGGGCTATAAAGGCAAGGACCT
  GTGCCAGAGCAGCCACCATAAATGCCGGGTGATCCTGGGCCAGTGGCTTGGCTTCTCTCATGATGTCCCC
  ACTATGGCTGGGGAGGAATGGAAGCATGGAGGTGATCAATCTCCCCCCAGGATCCATACCCCCAGGAGAA
  GAGGCCTAAGAGAGAAGGCCTGCAAAGGAGACAAAACAAAGGAGGCAGTGAGCATCTTGAAACATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229273 representing NM_001109809
  Red=Cloning site Green=Tags(s)
  
  MFEQLKPIEPVQKTLPWVGEVAATLQEAMKRDCWREARVKKKPVTFEDVAVNFTQEEWDCLDASQRVLYQ
  DVMSETFKNLTSVARIFLHKPELITKLEQEEEQWREFVHLPNTEGLSEGKKKELREQHPSLRDEGTSDDK
  VFLACRGAGQCPLSAPAGTMDRTRVLQASQAGPPFFCYTCGKCFSRRSYLYSHQFVHNPKLTNSCSQCGK
  LFRSPKSLSYHRRMHLGERPFCCTLCDKTYCDASGLSRHRRVHLGYRPHSCSVCGKSFRDQSELKRHQKI
  HQNQEPVDGNQECTLRIPGTQAEFQTPIARSQRSIQGLLDVNHAPVARSQEPIFRTEGPMAQNQASVLKN
  QAPVTRTQAPITGTLCQDARSNSHPVKPSRLNVFCCPHCSLTFSKKSYLSRHQKAHLTEPPNYCFHCSKS
  FSSFSRLVRHQQTHWKQKSYLCPICDLSFGEKEGLMDHWRGYKGKDLCQSSHHKCRVILGQWLGFSHDVP
  TMAGEEWKHGGDQSPPRIHTPRRRGLREKACKGDKTKEAVSILKHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109809
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001109809.2, NP_001103279.2
• RefSeq ORF: 1611
• Locus ID: 346171
• Protein Families: ES Cell Differentiation/IPS
• MW: 61.2 kDa
• Gene Summary: The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1). [provided by RefSeq, Sep 2009]