ARHGAP21 (NM_020824) Human Tagged ORF Clone

CAT#: RC229458

ARHGAP21 (Myc-DDK-tagged)-Human Rho GTPase activating protein 21 (ARHGAP21)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP21
• Synonyms: ARHGAP10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229458 representing NM_020824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCACGCGTCGGACTGGTCTGTCTGAGGGAGATGGTGACAAGCTCAAGGCCTGCGAGGTCTCAA
  AAAATAAAGATGGAAAAGAACAAAGTGAAACTGTATCACTGTCTGAAGATGAAACATTCTCCTGGCCAGG
  TCCCAAAACAGTTACGTTGAAAAGAACATCTCAAGGCTTTGGTTTTACATTAAGACATTTTATTGTTTAT
  CCCCCAGAGTCTGCAATTCAATTTTCATATAAGGATGAAGAAAATGGAAACAGAGGAGGAAAACAAAGAA
  ACCGCTTGGAACCAATGGATACCATATTTGTTAAGCAAGTTAAAGAAGGAGGACCTGCTTTTGAAGCTGG
  ATTATGTACAGGTGACCGAATTATAAAAGTCAATGGAGAAAGTGTTATTGGCAAAACCTATTCCCAAGTA
  ATTGCTTTAATTCAAAACAGTGATACAACATTGGAACTTAGTGTTATGCCAAAAGATGAAGACATTCTCC
  AAGTGCTACAGTTTACAAAGGATGTCACAGCACTGGCATATTCTCAAGATGCCTACCTGAAAGGCAACGA
  AGCTTATAGCGGCAATGCCCGCAATATACCTGAACCTCCACCAATCTGCTATCCCTGGCTGCCATCTGCC
  CCATCAGCCATGGCACAGCCAGTTGAAATATCTCCTCCTGACTCATCATTGAGCAAACAGCAAACCAGTA
  CACCAGTACTGACACAACCTGGTAGGGCCTATAGAATGGAAATACAAGTGCCTCCATCACCAACAGATGT
  TGCAAAATCAAACACAGCAGTGTGTGTTTGCAATGAAAGTGTAAGGACTGTCATTGTGCCTTCTGAGAAG
  GTTGTAGATTTGTTATCCAATAGAAACAACCATACAGGTCCTTCACATAGAACTGAAGAAGTGAGGTATG
  GCGTGAGTGAGCAGACCTCTTTAAAAACAGTGTCAAGAACCACATCACCACCATTATCAATTCCCACCAC
  TCATCTAATTCATCAGCCTGCAGGCTCCAGATCACTGGAACCTTCTGGAATTTTACTTAAGTCTGGAAAT
  TACAGTGGACATTCTGATGGAATCTCAAGCAGCAGATCTCAAGCTGTGGAGGCTCCCTCTGTATCTGTTA
  ATCACTATTCGCCAAATTCCCATCAGCACATAGACTGGAAAAACTATAAAACTTACAAAGAGTATATTGA
  TAACAGACGATTGCACATAGGTTGTCGGACAATACAAGAAAGATTAGATAGTTTAAGAGCAGCATCTCAA
  AGCACGACAGATTATAACCAGGTCGTCCCCAACCGCACTACTTTGCAGGGACGACGTCGAAGCACCTCTC
  ATGATCGAGTGCCCCAGTCTGTCCAGATACGGCAACGCAGTGTGTCCCAAGAAAGACTGGAAGATTCTGT
  GCTAATGAAGTATTGTCCAAGAAGTGCATCTCAAGGAGCACTGACGTCTCCATCTGTTAGTTTTAGTAAT
  CATAGAACTCGTTCATGGGATTATATTGAGGGACAGGATGAAACCTTAGAAAATGTCAATTCTGGAACTC
  CAATACCTGATTCCAATGGAGAGAAAAAACAGACTTACAAGTGGAGTGGGTTTACTGAACAGGATGATAG
  ACGAGGTATTTGTGAAAGACCTAGGCAGCAAGAAATTCATAAATCTTTTCGAGGTTCCAATTTTACTGTG
  GCTCCAAGCGTTGTTAATTCTGATAACAGGCGAATGAGTGGTAGAGGAGTGGGATCTGTGTCGCAGTTTA
  AAAAAATTCCACCAGATCTAAAAACATTGCAGTCAAACAGAAATTTTCAGACTACTTGTGGAATGTCACT
  GCCTCGGGGTATTTCACAAGACAGGTCACCTCTTGTGAAAGTCCGAAGTAATTCTCTGAAAGCTCCTTCC
  ACGCATGTCACAAAACCATCATTTAGCCAGAAATCATTTGTTTCTATCAAAGACCAAAGACCAGTAAATC
  ACTTGCATCAGAACAGTCTGTTGAATCAGCAGACATGGGTAAGGACTGACAGTGCCCCCGATCAGCAAGT
  GGAGACTGGGAAATCCCCCTCTTTATCTGGAGCCTCTGCCAAGCCTGCCCCTCAGTCGAGTGAAAACGCT
  GGTACTTCAGATTTAGAACTACCTGTCAGTCAAAGGAATCAAGATTTAAGTTTACAAGAGGCTGAAACTG
  AGCAATCAGATACTTTAGATAATAAAGAAGCTGTCATCCTAAGGGAAAAACCTCCATCTGGACGCCAGAC
  ACCGCAGCCTTTAAGGCATCAGTCTTACATCTTGGCAGTAAATGACCAGGAGACCGGGTCAGACACTACC
  TGCTGGCTGCCCAATGATGCACGTCGAGAGGTCCACATAAAAAGAATGGAGGAAAGAAAAGCCTCGAGTA
  CCAGTCCGCCTGGCGATTCTTTGGCTTCCATCCCATTTATAGATGAACCAACTAGCCCTAGCATTGATCA
  TGATATTGCACATATCCCTGCCTCTGCTGTTATATCAGCCTCTACCTCTCAGGTCCCCTCCATAGCAACA
  GTTCCTCCTTGCCTCACAACTTCAGCTCCATTAATTCGCCGTCAGCTCTCACATGACCACGAATCTGTTG
  GCCCTCCTAGCCTGGATGCTCAGCCCAACTCAAAGACAGAAAGATCAAAATCATATGATGAGGGTCTGGA
  TGATTACAGAGAAGATGCAAAATTGTCCTTTAAGCACGTATCTAGTCTGAAGGGAATCAAGATCGCAGAC
  AGCCAAAAGTCATCAGAAGACTCTGGGTCCAGAAAAGATTCTTCCTCAGAGGTCTTCAGTGATGCTGCCA
  AGGAAGGGTGGCTTCATTTCCGACCCCTTGTCACCGATAAGGGCAAGCGAGTTGGTGGAAGTATTCGGCC
  ATGGAAACAGATGTATGTTGTCCTTCGGGGTCATTCACTTTACCTGTACAAAGATAAAAGAGAGCAGACG
  ACTCCGTCTGAGGAAGAGCAGCCCATCAGTGTTAATGCTTGCTTGATAGACATCTCTTACAGTGAGACCA
  AGAGGAAAAATGTGTTTCGACTCACCACGTCCGACTGTGAATGCCTGTTTCAGGCTGAAGACAGAGATGA
  TATGCTAGCTTGGATCAAGACGATCCAGGAGAGCAGCAACCTAAACGAAGAGGACACTGGAGTCACTAAC
  AGGGATCTAATTAGTCGAAGAATAAAAGAATACAACAATCTGATGAGCAAAGCAGAACAGTTGCCAAAAA
  CACCTCGCCAGAGTCTCAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCC
  ACACTCTCCGAAGGAAGAGTCGGAAAGGAAACTTCTCAGTAAAGATGATACCAGTCCCCCAAAAGACAAA
  GGCACATGGAGAAAAGGCATTCCAAGTATCATGAGAAAGACATTTGAGAAAAAGCCAACTGCTACAGGAA
  CTTTCGGCGTCCGACTAGATGACTGCCCACCAGCTCATACTAATCGGTATATTCCATTAATAGTTGACAT
  ATGTTGCAAATTAGTTGAAGAAAGAGGTCTTGAATATACAGGTATTTATAGAGTTCCTGGAAATAATGCA
  GCCATCTCAAGTATGCAAGAAGAACTCAACAAGGGAATGGCTGATATTGATATACAAGATGATAAATGGC
  GAGATTTGAATGTGATAAGCAGTTTACTAAAATCCTTCTTCAGAAAACTCCCTGAGCCTCTCTTCACAAA
  TGATAAATATGCTGATTTTATTGAAGCCAATCGTAAAGAAGATCCTCTAGATCGTCTGAAAACATTAAAA
  AGACTAATTCACGATTTGCCTGAACATCATTATGAAACACTTAAGTTCCTTTCAGCTCATCTGAAGACAG
  TGGCAGAAAATTCAGAAAAAAATAAGATGGAACCAAGAAACCTAGCAATAGTGTTTGGTCCCACCCTTGT
  TCGAACATCAGAAGACAACATGACCCACATGGTCACCCACATGCCTGACCAGTACAAGATTGTAGAAACG
  CTCATCCAGCACCATGACTGGTTTTTCACAGAAGAAGGTGCTGAAGAGCCTCTTACAACAGTGCAGGAGG
  AAAGCACAGTAGACTCCCAGCCAGTGCCAAACATAGATCATTTACTCACCAACATTGGAAGGACAGGAGT
  CTCCCCAGGAGATGTATCAGATTCAGCTACTAGTGACTCAACAAAATCTAAGGGTTCTTGGGGATCTGGA
  AAGGATCAGTATAGCAGGGAACTGCTTGTGTCCTCCATCTTTGCAGCTGCTAGTCGCAAGAGGAAGAAGC
  CGAAAGAAAAAGCACAGCCTAGCAGCTCAGAAGATGAACTGGACAATGTATTTTTTAAGAAAGAAAATGT
  GGAACAGTGTCACAATGATACTAAAGAGGAGTCCAAAAAAGAAAGTGAGACACTGGGCAGAAAACAGAAG
  ATCATCATTGCCAAAGAAAACAGCACTAGGAAAGACCCCAGCACGACAAAAGATGAAAAGATATCACTAG
  GAAAAGAGAGCACGCCTTCTGAAGAACCCTCACCACCACACAACTCAAAACACAACAAGTCACCAACTCT
  CAGCTGTCGCTTTGCCATCCTGAAAGAGAGCCCCAGGTCACTTCTGGCACAGAAGTCCTCCCACCTTGAA
  GAGACAGGCTCTGACTCTGGCACTTTGCTCAGCACGTCTTCCCAGGCCTCCCTGGCAAGGTTTTCCATGA
  AGAAATCAACCAGTCCAGAAACGAAACATAGCGAGTTTTTGGCCAACGTCAGCACCATCACCTCAGATTA
  TTCCACCACATCGTCTGCTACATACTTGACTAGCCTGGACTCCAGTCGACTGAGCCCTGAGGTGCAATCC
  GTGGCAGAGAGCAAGGGGGACGAGGCAGATGACGAGAGAAGCGAACTCATCAGTGAAGGGCGGCCTGTGG
  AAACCGACAGCGAGAGCGAGTTTCCCGTGTTCCCCACAGCCTTGACTTCAGAGAGGCTTTTCCGAGGAAA
  ACTGCAAGAAGTGACTAAGAGCAGCCGGAGAAATTCTGAAGGAAGTGAATTAAGTTGCACCGAGGGAAGT
  TTAACATCAAGTTTAGATAGCCGGAGACAGCTCTTCAGTTCCCATAAACTCATCGAATGTGATACTCTTT
  CCAGGAAAAAATCAGCTAGATTCAAGTCAGATAGTGGAAGTCTAGGAGATGCCAAGAATGAGAAAGAAGC
  ACCTTCGTTAACTAAAGTGTTTGATGTTATGAAAAAAGGAAAGTCAACTGGGAGTTTACTGACACCCACC
  AGAGGCGAATCCGAAAAACAGGAACCCACATGGAAAACGAAAATAGCAGATCGGTTAAAACTGAGACCCA
  GAGCCCCTGCGGATGACATGTTTGGAGTAGGGAATCACAAAGTGAATGCCGAGACTGCTAAAAGGAAAAG
  CATCCGGCGCAGACATACACTAGGAGGGCACAGAGATGCTACCGAAATCAGCGTTTTGAATTTTTGGAAA
  GTGCATGAGCAGAGCGGGGAGAGAGAATCTGAACTTTCAGCTGTAAACCGGTTAAAACCAAAATGCTCAG
  CCCAGGACCTTTCCATCTCAGACTGGCTGGCCAGGGAACGCCTACGCACCAGTACCTCTGACCTTAGCAG
  AGGAGAAATCGGAGATCCCCAGACAGAGAACCCAAGCACACGAGAAATAGCCACGACCGACACACCTTTG
  TCTCTTCATTGCAACACAGGCAGTTCTTCCAGCACCTTGGCTTCAACAAACAGGCCCCTTCTTTCCATAC
  CACCACAGTCACCTGACCAAATAAACGGAGAAAGCTTCCAGAACGTGAGCAAAAATGCTAGTTCTGCAGC
  GAATGCCCAACCTCATAAACTGTCTGAAACCCCAGGCAGTAAAGCAGAGTTTCATCCCTGTCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229458 representing NM_020824
  Red=Cloning site Green=Tags(s)
  
  MMATRRTGLSEGDGDKLKACEVSKNKDGKEQSETVSLSEDETFSWPGPKTVTLKRTSQGFGFTLRHFIVY
  PPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQV
  IALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPICYPWLPSA
  PSAMAQPVEISPPDSSLSKQQTSTPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEK
  VVDLLSNRNNHTGPSHRTEEVRYGVSEQTSLKTVSRTTSPPLSIPTTHLIHQPAGSRSLEPSGILLKSGN
  YSGHSDGISSSRSQAVEAPSVSVNHYSPNSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQ
  STTDYNQVVPNRTTLQGRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPSVSFSN
  HRTRSWDYIEGQDETLENVNSGTPIPDSNGEKKQTYKWSGFTEQDDRRGICERPRQQEIHKSFRGSNFTV
  APSVVNSDNRRMSGRGVGSVSQFKKIPPDLKTLQSNRNFQTTCGMSLPRGISQDRSPLVKVRSNSLKAPS
  THVTKPSFSQKSFVSIKDQRPVNHLHQNSLLNQQTWVRTDSAPDQQVETGKSPSLSGASAKPAPQSSENA
  GTSDLELPVSQRNQDLSLQEAETEQSDTLDNKEAVILREKPPSGRQTPQPLRHQSYILAVNDQETGSDTT
  CWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHDIAHIPASAVISASTSQVPSIAT
  VPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKIAD
  SQKSSEDSGSRKDSSSEVFSDAAKEGWLHFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDKREQT
  TPSEEEQPISVNACLIDISYSETKRKNVFRLTTSDCECLFQAEDRDDMLAWIKTIQESSNLNEEDTGVTN
  RDLISRRIKEYNNLMSKAEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDK
  GTWRKGIPSIMRKTFEKKPTATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNA
  AISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLKTLK
  RLIHDLPEHHYETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVET
  LIQHHDWFFTEEGAEEPLTTVQEESTVDSQPVPNIDHLLTNIGRTGVSPGDVSDSATSDSTKSKGSWGSG
  KDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDNVFFKKENVEQCHNDTKEESKKESETLGRKQK
  IIIAKENSTRKDPSTTKDEKISLGKESTPSEEPSPPHNSKHNKSPTLSCRFAILKESPRSLLAQKSSHLE
  ETGSDSGTLLSTSSQASLARFSMKKSTSPETKHSEFLANVSTITSDYSTTSSATYLTSLDSSRLSPEVQS
  VAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGS
  LTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPT
  RGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWK
  VHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPL
  SLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGSKAEFHPCL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020824
• ORF Size: 5874 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020824.1, NM_020824.2, NM_020824.3, NP_065875.2, NP_065875.3
• RefSeq ORF: 5877
• Locus ID: 57584
• Domains: RhoGAP, PDZ, PH
• MW: 217.3 kDa
• Gene Summary: ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 (MIM 116952) and regulates the ARP2/3 complex (MIM 604221) and F-actin dynamics at the Golgi through control of CDC42 activity (Dubois et al., 2005 [PubMed 15793564]). [supplied by OMIM, Mar 2008]