ZFYVE27 (NM_001174122) Human Tagged ORF Clone

CAT#: RC229830

ZFYVE27 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 7

Product Images

Western blot validation of overexpression lysate (Cat# LY432830) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229830 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFYVE27
• Synonyms: PROTRUDIN; SPG33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229830 representing NM_001174122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG
  AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT
  GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTTCTATGGGGCT
  CTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGCACGC
  TCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGATGAA
  CCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCTGATG
  GACAGCACGCCTGCCCTCACACCCACGGAGGACCTCACACCGGGCAGCGTGGAGGAGGCTGAGGAGGCTG
  AGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGGATGATGAGGGCGCCCCGTGCCCAGCAGAGGATGA
  GCTGGCCCTGCAGGACAACGGGTTCCTGAGCAAGAATGAGGTGCTGCGCAGCAAGGTGTCTCGGCTCACG
  GAGCGGCTCCGCAAGCGCTACCCCACCAACAACTTCGGGAACTGCACGGGCTGCTCGGCCACCTTCTCAG
  TGCTGAAGAAGAGGCGGAGCTGCAGTAATTGTGGAAACAGCTTCTGCTCTCGATGCTGCTCCTTCAAGGT
  GCCCAAGTCCTCCATGGGGGCCACAGCCCCTGAAGCCCAGAGGGAGACTGTGTTTGTGTGTGCCTCGTGT
  AACCAGACCTTGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229830 representing NM_001174122
  Red=Cloning site Green=Tags(s)
  
  MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRFYGA
  LLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLM
  DSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLT
  ERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASC
  NQTLSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174122
• ORF Size: 858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001174122.1, NP_001167593.1
• RefSeq ORF: 861
• Locus ID: 118813
• Protein Families: Transmembrane
• MW: 32.1 kDa
• Gene Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]