LMX1A (NM_001174069) Human Tagged ORF Clone

CAT#: RC229968

LMX1A (Myc-DDK-tagged)-Human LIM homeobox transcription factor 1, alpha (LMX1A), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY432968) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229968 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LMX1A
• Synonyms: LMX1; LMX1.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229968 representing NM_001174069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACGGCCTAAAGATGGAGGAGAACTTCCAAAGCGCGATCGACACCTCGGCCTCCTTCTCCTCGC
  TGCTGGGCAGAGCGGTGAGCCCCAAGTCTGTCTGCGAGGGCTGTCAGCGGGTCATCTTGGACAGGTTTCT
  GCTGCGGCTCAACGACAGCTTCTGGCATGAGCAGTGCGTGCAGTGCGCCTCCTGCAAAGAGCCCCTGGAG
  ACCACCTGCTTCTACCGGGACAAGAAGCTGTACTGCAAGTATGACTACGAGAAGCTGTTTGCTGTTAAAT
  GTGGGGGCTGCTTCGAGGCCATCGCTCCCAATGAGTTTGTTATGCGGGCCCAGAAGAGTGTATACCACCT
  GAGCTGCTTCTGCTGCTGTGTCTGCGAGCGACAGCTTCAGAAGGGTGATGAGTTTGTCCTGAAGGAGGGG
  CAGCTGCTCTGCAAAGGGGACTATGAGAAGGAGCGGGAGCTGCTCAGCCTGGTGAGCCCAGCAGCCTCAG
  ACTCAGGTAAAAGTGATGATGAAGAAAGTCTCTGCAAGTCAGCCCATGGGGCAGGGAAAGGAACTGCTGA
  GGAAGGCAAGGACCATAAGCGCCCCAAACGTCCGAGAACCATCTTGACAACTCAACAGAGGCGAGCATTC
  AAGGCCTCATTTGAAGTATCCTCCAAGCCCTGCAGGAAGGTGAGAGAGACTCTGGCTGCAGAGACAGGGC
  TGAGTGTCCGTGTCGTCCAGGTGTGGTTCCAAAACCAGAGAGCGAAGATGAAGAAGCTGGCCAGGCGACA
  GCAGCAGCAGCAGCAAGATCAGCAGAACACCCAGAGGCTGAGCTCTGCTCAGACAAACGGTGGTGGGAGT
  GCTGGGATGGAAGGAATCATGAACCCCTACACGGCTCTGCCCACCCCACAGCAGCTCCTGGCCATCGAGC
  AGAGTGTCTACAGCTCAGATCCCTTCCGACAGGGTCTCACCCCACCCCAGATGCCTGGAGACCACATGCA
  CCCTTATGGTGCCGAGCCCCTTTTCCATGACCTGGATAGCGACGACACCTCCCTCAGTAACCTGGGTGAT
  TGTTTCCTAGCAACCTCAGAAGCTGGGCCTCTGCAGTCCAGAGTGGGAAACCCCATTGACCATCTGTACT
  CCATGCAGAATTCTTACTTCACATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229968 representing NM_001174069
  Red=Cloning site Green=Tags(s)
  
  MLDGLKMEENFQSAIDTSASFSSLLGRAVSPKSVCEGCQRVILDRFLLRLNDSFWHEQCVQCASCKEPLE
  TTCFYRDKKLYCKYDYEKLFAVKCGGCFEAIAPNEFVMRAQKSVYHLSCFCCCVCERQLQKGDEFVLKEG
  QLLCKGDYEKERELLSLVSPAASDSGKSDDEESLCKSAHGAGKGTAEEGKDHKRPKRPRTILTTQQRRAF
  KASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRQQQQQQDQQNTQRLSSAQTNGGGS
  AGMEGIMNPYTALPTPQQLLAIEQSVYSSDPFRQGLTPPQMPGDHMHPYGAEPLFHDLDSDDTSLSNLGD
  CFLATSEAGPLQSRVGNPIDHLYSMQNSYFTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174069
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174069.1, NP_001167540.1
• RefSeq ORF: 1149 bp
• Locus ID: 4009
• Cytogenetics: 1q23.3
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 43.2 kDa
• Gene Summary: 'This gene encodes a homeodomain and LIM-domain containing protein. The encoded protein is a transcription factor that acts as a positive regulator of insulin gene transcription. This gene also plays a role in the development of dopamine producing neurons during embryogenesis. Mutations in this gene are associated with an increased risk of developing Parkinson's disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]'