MPP1 (NM_001166461) Human Tagged ORF Clone

CAT#: RC230095

MPP1 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY433095) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230095 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPP1
• Synonyms: AAG12; DXS552E; EMP55; MRG1; PEMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230095 representing NM_001166461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCAAGGCGAGCGAGGGCGAGAGTGGGGGCAGCATGCACACGGCGCTCTCCGACCTCTACCTGG
  AGCATTTGCTGCAGAAGCGTAGTCGGCCAGAGGCTGTATCGCATCCATTGAATACTGTGACCGAGGACAT
  GTACACCAACGGGTCTCCTGCCCCAGGTAGCCCTGCCCAGGTCAAGGGACAGGAGGTGCGGAAAGTGCGA
  CTCATACAGTTTGAGAAGGTCACAGAAGAGCCCATGGGAATCACGCTGAAGCTGAATGAAAAACAGTCCT
  GTACGGTGGCCAGAATTCTTCATGGTGGCATGATCCATAGACAAGGCTCCCTTCACGTGGGGGATGAGAT
  CCTAGAAATCAATGGCACAAATGTGACAAATCATTCAGTGGATCAGCTGCAGAAGGCGATGAAAGAAACC
  AAAGGAATGATCTCATTAAAAGTAATTCCCAACCAGCAAAGCCGTCTTCCTGCACTACAGGAGGCGGGAC
  TGAAGTTTGCTACTGGGGACATTATCCAGATTATCAACAAGGATGACAGCAATTGGTGGCAGGGACGGGT
  GGAAGGCTCCTCCAAGGAGTCAGCAGGATTGATCCCTTCCCCTGAGCTGCAGGAATGGCGAGTGGCAAGT
  ATGGCTCAGTCAGCTCCTAGCGAAGCCCCGAGCTGCAGTCCCTTTGGGAAGAAGAAGAAGTACAAAGACA
  AATATCTGGCCAAGCACAGCTCGATTTTTGATCAGTTGGATGTTGTTTCCTACGAGGAAGTCGTTCGGCT
  CCCTGCATTCAAGAGGAAGACCCTGGTGCTGATCGGAGCCAGTGGGGTGGGTCGCAGCCACATTAAGAAT
  GCCCTGCTCAGCCAGAATCCGGAGAAGTTTGTGTACCCTGTCCCATATACAACACGGCCGCCAAGGAAGA
  GTGAGGAAGATGGGAAGGAGTACCACTTTATCTCAACGGAGGAGATGACGAGGAACATCTCTGCCAATGA
  GTTCTTGGAGTTTGGCAGCTACCAAGGCAACATGTTTGGCACCAAATTTGAAACAGTGCACCAGATCCAT
  AAGCAGAACAAGATTGCCATCCTTGACATTGAGCCCCAGACCCTGAAAATTGTTCGGACAGCAGAACTTT
  CGCCTTTCATTGTGTTCATTGCACCTACTGACCAGGGCACTCAGACAGAAGCCCTGCAGCAGCTGCAGAA
  GGACTCTGAGGCCATCCGCAGCCAGTACGCTCACTACTTTGACCTCTCACTGGTCAATAATGGTGTTGAT
  GAAACCCTTAAGAAATTACAAGAAGCCTTCGACCAAGCGTGCAGTTCTCCACAGTGGGTGCCTGTCTCCT
  GGGTTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230095 representing NM_001166461
  Red=Cloning site Green=Tags(s)
  
  MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVR
  LIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKET
  KGMISLKVIPNQQSRLPALQEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVAS
  MAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKN
  ALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIH
  KQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVD
  ETLKKLQEAFDQACSSPQWVPVSWVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166461
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166461.1, NP_001159933.1
• RefSeq ORF: 1341 bp
• Locus ID: 4354
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• MW: 50.3 kDa
• Gene Summary: 'This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]'