ZNF548 (NM_001172773) Human Tagged ORF Clone

CAT#: RC230255

ZNF548 (Myc-DDK-tagged)-Human zinc finger protein 548 (ZNF548), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF548
• Synonyms: FLJ32932
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230255 ORF sequence, codon optimized.
  Due to the complexity of NM_001172773, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCACTGAGGGTCCGCTTGCCATGGCCGAGATGGATCCTACACAGGGTAGGGTGGTTTTCGAAG
  ATGTGGCCATCTATTTTTCACAGGAAGAGTGGGGGCACCTTGACGAGGCACAGCGACTGCTGTATCGGGA
  CGTGATGCTCGAGAATTTGGCCCTCCTGAGTTCTCTTGGGTCCTGGCATGGGGCTGAAGATGAGGAGGCT
  CCTAGTCAACAAGGTTTTTCTGTAGGAGTTTCTGAGGTGACGGCCTCCAAGCCATGCCTGTCATCTCAAA
  AGGTGCATCCGTCTGAGACGTGTGGGCCACCTTTGAAAGATATTTTGTGTCTGGTGGAACATAATGGGAT
  TCACCCCGAGCAGCACATCTATATTTGTGAAGCTGAGTTGTTTCAGCACCCGAAGCAGCAGATCGGTGAG
  AATCTGTCTCGCGGGGACGATTGGATTCCCAGTTTTGGTAAGAATCACCGCGTTCACATGGCCGAGGAAA
  TTTTTACCTGTATGGAGGGGTGGAAGGACCTTCCAGCTACATCTTGCCTGCTCCAGCACCAGGGACCACA
  ATCAGAGTGGAAGCCCTATCGCGATACAGAGGATAGGGAAGCCTTCCAGACCGGCCAAAACGACTACAAG
  TGCTCTGAATGCGGCAAAACCTTTACTTGCTCATACTCTTTCGTCGAGCACCAGAAGATCCACACGGGGG
  AGCGGTCCTATGAGTGTAACAAATGCGGTAAATTTTTTAAGTACTCCGCTAATTTCATGAAGCACCAGAC
  TGTACACACATCTGAACGGACCTATGAGTGCCGGGAATGCGGTAAGAGCTTCATGTATAATTACCGCCTC
  ATGAGACATAAGCGAGTCCACACCGGGGAGAGGCCATATGAGTGCAATACCTGTGGCAAATTCTTTCGAT
  ACAGTTCTACATTTGTGCGCCATCAAAGAGTGCATACGGGAGAGCGCCCTTACGAGTGTAGAGAATGCGG
  AAAGTTCTTTATGGATTCATCCACCTTGATTAAGCACCAGCGAGTGCACACAGGCGAACGGCCATACAAA
  TGCAATGACTGTGGAAAGTTCTTTAGGTATATTTCTACGCTGATCAGGCACCAAAGAATTCACACCGGCG
  AAAGGCCTTACGAGTGCAGCGTTTGTGGCGAACTGTTCAGATATAACTCATCTTTGGTGAAGCACTGGCG
  CAACCATACCGGCGAAAGACCTTATAAATGCTCTGAGTGCGGCAAAAGTTTCAGGTATCACTGTCGACTG
  ATCAGACACCAAAGAGTTCACACCGGTGAAAGGCCGTACGAGTGCAGCGAGTGTGGTAAGTTTTTCAGAT
  ATAACTCAAACCTTATCAAACACTGGAGGAATCATACAGGGGAGAGGCCTTATGAATGTCGCGAGTGTGG
  TAAAGCTTTCAGCCACAAGCACATCCTCGTGGAGCACCAGAAGATCCACAGTGGAGAACGCCCTTATGAA
  TGTTCCGAGTGCCAGAAAGCCTTTATTCGGAAATCCCACTTGGTCCACCACCAGAAGATTCACTCCGAAG
  AAAGACTCGTATGTTCCATGAATGTAGGCAATAGCCTTGCCAAAACTCCAACTTCCCTGAACATCAGAGA
  TTTCACCATGGAGAAGGTCTACCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230255 representing NM_001172773
  Red=Cloning site Green=Tags(s)
  
  MNLTEGPLAMAEMDPTQGRVVFEDVAIYFSQEEWGHLDEAQRLLYRDVMLENLALLSSLGSWHGAEDEEA
  PSQQGFSVGVSEVTASKPCLSSQKVHPSETCGPPLKDILCLVEHNGIHPEQHIYICEAELFQHPKQQIGE
  NLSRGDDWIPSFGKNHRVHMAEEIFTCMEGWKDLPATSCLLQHQGPQSEWKPYRDTEDREAFQTGQNDYK
  CSECGKTFTCSYSFVEHQKIHTGERSYECNKCGKFFKYSANFMKHQTVHTSERTYECRECGKSFMYNYRL
  MRHKRVHTGERPYECNTCGKFFRYSSTFVRHQRVHTGERPYECRECGKFFMDSSTLIKHQRVHTGERPYK
  CNDCGKFFRYISTLIRHQRIHTGERPYECSVCGELFRYNSSLVKHWRNHTGERPYKCSECGKSFRYHCRL
  IRHQRVHTGERPYECSECGKFFRYNSNLIKHWRNHTGERPYECRECGKAFSHKHILVEHQKIHSGERPYE
  CSECQKAFIRKSHLVHHQKIHSEERLVCSMNVGNSLAKTPTSLNIRDFTMEKVYH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172773
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172773.1, NP_001166244.1
• RefSeq Size: 4987
• RefSeq ORF: 1638
• Locus ID: 147694
• Protein Families: Transcription Factors
• MW: 64 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]