SATB2 (NM_001172517) Human Tagged ORF Clone

CAT#: RC230467

SATB2 (Myc-DDK-tagged)-Human SATB homeobox 2 (SATB2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SATB2
• Synonyms: GLSS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230467 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGAGCGGCGGAGCGAGAGCCCGTGTCTGCGGGACAGCCCCGACCGGCGGAGCGGCAGCCCGGACGT
  CAAGGGGCCTCCCCCAGTGAAGGTGGCCCGGCTGGAGCAGAACGGCAGCCCCATGGGAGCCCGCGGGAGG
  CCCAACGGCGCCGTGGCCAAGGCCGTGGGAGGTTTGATGATTCCTGTCTTTTGTGTCGTGGAGCAGTTGG
  ACGGCTCTCTTGAATATGACAACAGAGAAGAACACGCCGAGTTTGTCCTGGTGCGGAAAGATGTGCTTTT
  TAGCCAGCTGGTGGAGACTGCGCTCCTGGCCCTGGGGTATTCTCACAGCTCTGCGGCCCAGGCCCAAGGA
  ATAATCAAGCTGGGAAGGTGGAACCCTCTCCCCCTCAGTTATGTGACAGATGCACCCGACGCGACAGTGG
  CCGACATGCTACAAGATGTCTATCATGTTGTGACGTTGAAAATCCAATTACAAAGTTGTTCAAAGTTGGA
  AGACTTGCCTGCGGAGCAGTGGAACCATGCCACAGTCCGCAATGCCTTAAAGGAACTGCTCAAAGAGATG
  AACCAGAGCACATTAGCCAAAGAATGCCCTCTCTCCCAGAGTATGATTTCATCCATTGTAAATAGCACAT
  ATTATGCCAATGTGTCAGCAACCAAGTGCCAGGAGTTTGGGAGATGGTATAAAAAGTACAAGAAGATTAA
  AGTGGAAAGAGTGGAACGAGAAAACCTTTCAGACTATTGTGTTCTGGGCCAGCGTCCAATGCATTTACCA
  AATATGAACCAGCTGGCATCCCTGGGGAAAACCAACGAACAGTCTCCTCACAGCCAAATTCACCACAGTA
  CTCCAATCCGAAACCAAGTGCCCGCATTACAGCCCATCATGAGCCCTGGTCTTCTTTCTCCCCAGCTTAG
  TCCACAACTTGTAAGGCAACAAATAGCCATGGCCCATCTGATAAACCAACAGATTGCCGTTAGCCGGCTC
  CTGGCTCACCAGCATCCTCAAGCCATCAACCAGCAGTTCCTGAACCATCCACCCATCCCCAGAGCAGTTA
  AGCCAGAGCCAACCAACTCTTCCGTGGAAGTCTCTCCAGATATCTACCAGCAAGTCAGAGATGAGCTGAA
  GAGGGCCAGTGTGTCCCAAGCTGTCTTTGCAAGAGTGGCATTCAACCGCACACAGGGATTGTTGTCTGAG
  ATTCTGCGTAAGGAAGAAGACCCTCGGACAGCCTCTCAGTCTCTTCTAGTAAACCTGAGGGCCATGCAGA
  ATTTCCTCAATCTGCCAGAAGTGGAGCGAGATCGCATCTACCAGGATGAGAGGGAGCGGAGCATGAATCC
  CAATGTGAGCATGGTCTCCTCGGCCTCCAGCAGTCCCAGCTCCTCCCGAACCCCTCAGGCCAAAACCTCG
  ACACCGACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACATCAACATCACAGCTGCCATTTATGACG
  AGATCCAACAGGAGATGAAAAGGGCCAAGGTGTCTCAAGCCCTGTTTGCCAAAGTGGCTGCAAATAAAAG
  TCAGGGCTGGCTGTGTGAACTGCTCCGCTGGAAGGAGAACCCAAGCCCAGAAAACCGCACCCTCTGGGAA
  AACCTCTGTACCATCCGTCGCTTCCTGAACCTTCCCCAGCATGAGAGGGATGTCATCTATGAGGAGGAGT
  CAAGGCATCACCACAGCGAACGCATGCAACACGTGGTCCAGCTTCCCCCTGAGCCGGTGCAGGTACTTCA
  TAGACAGCAGTCTCAGCCAGCCAAGGAGAGTTCCCCTCCCAGAGAAGAAGCGCCTCCCCCACCTCCTCCG
  ACTGAAGACAGTTGTGCCAAAAAGCCCCGGTCTCGCACAAAGATCTCCTTAGAAGCCCTGGGGATCCTCC
  AAAGCTTTATTCATGATGTAGGCCTGTACCCAGACCAGGAAGCCATCCACACTCTTTCGGCTCAGCTGGA
  TCTCCCCAAACACACCATCATCAAGTTCTTCCAGAACCAGCGGTACCACGTGAAGCACCACGGGAAGCTG
  AAAGAGCACCTGGGCTCCGCGGTGGACGTGGCTGAATATAAGGACGAGGAGCTGCTGACCGAGTCAGAGG
  AGAACGACAGCGAGGAAGGCTCCGAGGAGATGTACAAAGTGGAGGCTGAGGAGGAAAATGCTGACAAAAG
  CAAGGCAGCACCTGCCGAAATTGACCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230467 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWSGGARARVCGTAPTGGAAARTSRGLPQ*RWPGWSRTAAPWEPAGGPTAPWPRPWEV**FLSFVSWSSW
  TALLNMTTEKNTPSLSWCGKMCFLASWWRLRSWPWGILTALRPRPKE*SSWEGGTLSPSVM*QMHPTRQW
  PTCYKMSIML*R*KSNYKVVQSWKTCLRSSGTMPQSAMP*RNCSKR*TRAH*PKNALSPRV*FHPL*IAH
  IMPMCQQPSARSLGDGIKSTRRLKWKEWNEKTFQTIVFWASVQCIYQI*TSWHPWGKPTNSLLTAKFTTV
  LQSETKCPHYSPS*ALVFFLPSLVHNL*GNK*PWPI**TNRLPLAGSWLTSILKPSTSSS*TIHPSPEQL
  SQSQPTLPWKSLQISTSKSEMS*RGPVCPKLSLQEWHSTAHRDCCLRFCVRKKTLGQPLSLF**T*GPCR
  ISSICQKWSEIASTRMRGSGA*IPM*AWSPRPPAVPAPPEPLRPKPRHRQQTSLLRWTAPTSTSQLPFMT
  RSNRR*KGPRCLKPCLPKWLQIKVRAGCVNCSAGRRTQAQKTAPSGKTSVPSVAS*TFPSMRGMSSMRRS
  QGITTANACNTWSSFPLSRCRYFIDSSLSQPRRVPLPEKKRLPHLLRLKTVVPKSPGLAQRSP*KPWGSS
  KALFMM*ACTQTRKPSTLFRLSWISPNTPSSSSSRTSGTT*STTGS*KSTWAPRWTWLNIRTRSC*PSQR
  RTTARKAPRRCTKWRLRRKMLTKARQHLPKLTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172517
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172517.1, NP_001165988.1
• RefSeq Size: 5326 bp
• RefSeq ORF: 2202 bp
• Locus ID: 23314
• Protein Families: Transcription Factors
• MW: 82.6 kDa
• Gene Summary: This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]