ZFX (NM_001178085) Human Tagged ORF Clone

CAT#: RC230504

ZFX (Myc-DDK-tagged)-Human zinc finger protein, X-linked (ZFX), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFX
• Synonyms: ZNF926
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230504 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGATGAAGATGGGCTTGAATTACAACAAGAGCCAAACTCATTTTTTGATGCAACAGGAGCTGATGG
  TACACACATGGATGGTGATCAAATTGTTGTGGAAGTACAAGAAACTGTTTTTGTTTCAGATGTTGTGGAT
  TCAGACATAACTGTGCATAACTTTGTTCCTGATGACCCAGATTCAGTTGTAATCCAAGATGTTATTGAGG
  ACGTTGTTATAGAAGATGTTCAGTGCCCAGATATCATGGAAGAAGCAGATGTGTCTGAAACGGTCATCAT
  TCCTGAGCAAGTGCTGGACTCAGATGTAACTGAAGAAGTTTCTTTAGCACATTGCACAGTCCCAGATGAT
  GTTTTAGCTTCTGACATTACTTCAGCCTCAATGTCTATGCCAGAACACGTCTTGACGGGTGATTCTATAC
  ATGTGTCTGACGTTGGACATGTTGGACATGTTGGACATGTTGAACATGTGGTTCATGATAGTGTAGTGGA
  AGCAGAAATTGTCACTGATCCTCTGACTACCGACGTAGTTTCAGAAGAAGTATTGGTAGCAGACTGTGCC
  TCTGAAGCAGTCATAGATGCCAATGGGATCCCTGTGGACCAGCAGGATGATGACAAAGGCAACTGTGAGG
  ACTACCTTATGATTTCCTTGGATGATGCTGGCAAAATAGAACACGATGGTTCTTCTGGAATGACCATGGA
  CACAGAGTCGGAAATTGATCCTTGTAAAGTGGATGGCACTTGCCCTGAGGTCATCAAGGTGTACATTTTT
  AAAGCTGACCCTGGAGAAGATGACTTAGGTGGAACTGTAGACATTGTGGAGAGTGAGCCTGAGAATGATC
  ATGGAGTTGAACTGCTTGATCAGAACAGCAGTATTCGTGTTCCCAGGGAAAAGATGGTTTATATGACTGT
  CAATGACTCTCAGCCAGAAGATGAAGATTTAAATGTTGCTGAAATCGCTGACGAAGTTTATATGGAAGTG
  ATCGTAGGAGAGGAGGATGCTGCAGCAGCAGCGGCAGCCGCCGCCGTGCACGAGCAGCAAATGGATGACA
  ATGAAATCAAAACCTTCATGCCGATTGCATGGGCAGCAGCTTATGGTAATAATTCTGATGGAATTGAAAA
  CCGGAATGGCACTGCAAGTGCCCTCTTGCACATAGATGAGTCTGCTGGCCTCGGCAGACTGGCTAAACAA
  AAACCAAAGAAAAGGAGAAGACCTGATTCCAGGCAGTACCAAACAGCAATAATTATTGGCCCTGATGGAC
  ATCCTTTGACTGTCTATCCTTGCATGATTTGTGGGAAGAAGTTTAAGTCGAGAGGTTTTTTGAAAAGGCA
  CATGAAAAACCATCCCGAACACCTTGCCAAGAAGAAATACCGCTGTACTGACTGTGATTACACTACCAAC
  AAGAAGATAAGTTTACACAACCACCTGGAGAGCCACAAGCTGACCAGCAAGGCAGAGAAGGCCATTGAAT
  GCGATGAGTGTGGGAAGCATTTCTCTCATGCAGGGGCTTTGTTTACTCACAAAATGGTGCATAAGGAAAA
  AGGAGCCAACAAAATGCACAAGTGTAAATTCTGTGAATACGAGACAGCTGAACAAGGGTTATTGAATCGC
  CACCTCTTGGCAGTCCACAGCAAGAACTTTCCTCATATTTGTGTGGAGTGTGGTAAGGGTTTTCGTCACC
  CGTCAGAGCTCAAAAAGCACATGAGAATCCATACTGGGGAGAAGCCGTACCAATGCCAGTACTGCGAATA
  TAGGTCTGCAGACTCTTCTAACTTGAAAACGCATGTCAAAACTAAGCATAGTAAAGAGATGCCATTCAAG
  TGTGACATTTGTCTTCTGACTTTCTCGGATACCAAAGAGGTGCAGCAACATGCTCTTATCCACCAAGAAA
  GCAAAACACACCAGTGTTTGCATTGCGACCACAAGAGTTCGAACTCAAGTGATTTGAAACGACACATAAT
  TTCAGTTCACACGAAAGACTACCCCCATAAGTGTGACATGTGTGATAAAGGCTTTCACAGGCCTTCAGAA
  CTCAAGAAACACGTGGCTGCCCACAAGGGCAAAAAAATGCACCAGTGTAGACATTGTGACTTTAAGATTG
  CAGATCCATTTGTTCTAAGTCGCCATATTCTCTCAGTTCACACAAAGGATCTTCCATTTAGGTGCAAGAG
  ATGTAGAAAGGGATTTAGGCAACAGAGTGAGCTTAAAAAGCATATGAAGACACACAGTGGCAGGAAAGTG
  TATCAGTGTGAGTACTGTGAGTATAGCACTACAGATGCCTCAGGCTTTAAACGGCACGTTATTTCCATTC
  ACACGAAAGACTATCCTCACCGGTGTGAGTACTGCAAGAAAGGCTTCCGAAGACCTTCAGAAAAGAACCA
  GCACATAATGCGACATCATAAAGAAGTTGGCCTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230504 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWMKMGLNYNKSQTHFLMQQELMVHTWMVIKLLWKYKKLFLFQMLWIQT*LCITLFLMTQIQL*SKMLLR
  TLL*KMFSAQISWKKQMCLKRSSFLSKCWTQM*LKKFL*HIAQSQMMF*LLTLLQPQCLCQNTS*RVILY
  MCLTLDMLDMLDMLNMWFMIV*WKQKLSLIL*LPT*FQKKYW*QTVPLKQS*MPMGSLWTSRMMTKATVR
  TTL*FPWMMLAK*NTMVLLE*PWTQSRKLILVKWMALALRSSRCTFLKLTLEKMT*VEL*TLWRVSLRMI
  MELNCLIRTAVFVFPGKRWFI*LSMTLSQKMKI*MLLKSLTKFIWK*S*ERRMLQQQRQPPPCTSSKWMT
  MKSKPSCRLHGQQLMVIILMELKTGMALQVPSCT*MSLLASADWLNKNQRKGEDLIPGSTKQQ*LLALMD
  IL*LSILA*FVGRSLSREVF*KGT*KTIPNTLPRRNTAVLTVITLPTRR*VYTTTWRATS*PARQRRPLN
  AMSVGSISLMQGLCLLTKWCIRKKEPTKCTSVNSVNTRQLNKGY*IATSWQSTARTFLIFVWSVVRVFVT
  RQSSKST*ESILGRSRTNASTANIGLQTLLT*KRMSKLSIVKRCHSSVTFVF*LSRIPKRCSNMLLSTKK
  AKHTSVCIATTRVRTQVI*NDT*FQFTRKTTPISVTCVIKAFTGLQNSRNTWLPTRAKKCTSVDIVTLRL
  QIHLF*VAIFSQFTQRIFHLGARDVERDLGNRVSLKSI*RHTVAGKCISVSTVSIALQMPQALNGTLFPF
  TRKTILTGVSTARKASEDLQKRTST*CDIIKKLAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178085
• ORF Size: 2415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178085.1, NP_001171556.1
• RefSeq Size: 7740 bp
• RefSeq ORF: 2418 bp
• Locus ID: 7543
• Cytogenetics: Xp22.11
• Protein Families: Transcription Factors
• MW: 90.5 kDa
• Gene Summary: 'This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2010]'