OBSL1 (NM_001173408) Human Tagged ORF Clone

CAT#: RC230623

OBSL1 (Myc-DDK-tagged)-Human obscurin-like 1 (OBSL1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OBSL1
• Synonyms: KIAA0657; MGC71026
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230623 representing NM_001173408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCGAGCTCGGGGGATCAGGGGAGCCCCCCGTGCTTCCTGCGCTTCCCGCGGCCTGTGCGGGTGG
  TAAGTGGCGCCGAGGCCGAGCTCAAGTGCGTGGTCCTGGGGGAGCCGCCGCCTGTAGTGGTGTGGGAGAA
  GGGCGGGCAGCAGCTGGCGGCCTCGGAACGCCTGAGCTTCCCGGCGGACGGCGCGGAGCACGGCCTGCTG
  CTGACCGCCGCACTGCCCACCGACGCGGGGGTCTACGTGTGCCGCGCCCGCAACGCGGCCGGCGAGGCCT
  ACGCGGCGGCCGCCGTCACCGTGCTGGAGCCGCCGGCCTCCGACCCCGAGCTGCAGCCCGCCGAGCGCCC
  GCTGCCATCGCCGGGGTCCGGGGAGGGCGCCCCGGTCTTCCTCACGGGGCCTCGATCCCAGTGGGTGCTG
  CGGGGGGCGGAGGTGGTGCTGACGTGCCGGGCGGGGGGCCTCCCCGAGCCCACACTGTACTGGGAGAAGG
  ACGGGATGGCCCTGGACGAAGTGTGGGACAGCAGCCACTTCGCGCTCCAGCCGGGCCGCGCCGAGGACGG
  CCCCGGCGCGAGCCTGGCACTGCGCATCCTGGCGGCTCGGCTGCCGGATTCCGGCGTCTACGTGTGCCAC
  GCCCGCAACGCGCACGGCCACGCGCAGGCGGGGGCGCTGCTCCAGGTGCACCAGCCCCCCGAGAGCCCGC
  CCGCGGACCCCGACGAGGCCCCCGCGCCGGTGGTGGAGCCGCTCAAGTGCGCGCCTAAGACCTTCTGGGT
  GAACGAGGGCAAGCACGCCAAGTTCCGCTGCTACGTGATGGGCAAGCCCGAGCCCGAGATCGAATGGCAC
  TGGGAGGGCCGCCCGCTGCTCCCGGACCGCCGCCGCCTCATGTACCGCGACCGCGACGGCGGCTTCGTGC
  TCAAGGTGCTTTACTGCCAGGCCAAGGATCGTGGGCTCTACGTCTGCGCCGCGCGCAACTCGGCGGGCCA
  GACGCTCAGTGCCGTGCAGCTGCACGTGAAAGAGCCCCGCCTCCGGTTCACACGGCCCCTGCAGGACGTG
  GAGGGCCGTGAGCACGGGATTGCCGTGCTGGAATGTAAAGTACCCAACTCCCGCATCCCCACGGCCTGGT
  TCCGTGAGGACCAGCGGCTGCTGCCCTGCCGCAAGTACGAGCAGATCGAAGAGGGCACTGTCCGGCGCCT
  CATCATCCACAGGCTGAAGGCAGACGATGATGGTATCTACCTGTGCGAGATGCGGGGCCGGGTGCGCACC
  GTGGCCAACGTCACAGTCAAAGGGCCCATCCTGAAGCGCCTGCCCCGGAAGCTCGACGTCCTGGAAGGAG
  AGAATGCTGTGCTGCTAGTGGAAACTCTAGAGGCCGGGGTCGAGGGACGCTGGAGCCGTGATGGGGAGGA
  GCTGCCGGTCATCTGCCAGAGCAGCTCAGGCCACATGCATGCCCTGGTCCTTCCAGGGGTCACCCGAGAG
  GATGCTGGCGAGGTCACCTTTAGCCTGGGCAACTCCCGTACCACTACGCTTCTCAGAGTAAAATGTGTCA
  AGCACAGTCCCCCAGGACCCCCCATATTGGCAGAGATGTTCAAGGGCCACAAGAACACGGTCCTGTTGAC
  CTGGAAGCCTCCCGAGCCAGCTCCCGAGACCCCATTCATCTACCGGCTGGAGCGGCAGGAAGTGGGCTCT
  GAAGACTGGATTCAGTGCTTCAGCATCGAGAAAGCCGGAGCCGTGGAGGTGCCGGGCGACTGTGTGCCCT
  CCGAGGGTGACTACCGCTTCCGCATCTGCACAGTCAGCGGACATGGCCGTAGTCCCCACGTGGTGTTCCA
  CGGTTCTGCTCACCTTGTGCCCACAGCTCGCCTGGTGGCAGGTCTGGAGGATGTGCAGGTATACGACGGG
  GAAGATGCCGTCTTCTCCCTCGATCTCTCCACCATCATCCAGGGTACCTGGTTCCTTAATGGGGAAGAGC
  TCAAGAGTAACGAGCCGGAGGGCCAGGTGGAACCTGGGGCCCTGCGGTACCGTATAGAGCAGAAGGGTCT
  GCAGCACAGACTCATCCTGCATGCCGTCAAGCACCAGGACAGCGGTGCCCTGGTCGGCTTCAGCTGCCCC
  GGCGTGCAGGACTCAGCTGCCCTCACAATCCAAGAGAGCCCGGTGCACATCCTGAGCCCCCAGGACAGGG
  TGTCGTTGACCTTCACAACCTCAGAGCGGGTGGTGCTGACTTGTGAGCTCTCAAGGGTGGACTTCCCGGC
  AACCTGGTACAAGGATGGGCAGAAGGTGGAGGAGAGCGAGTTGCTGGTGGTGAAGATGGATGGGCGCAAA
  CACCGTCTGATCCTGCCTGAGGCCAAAGTCCAGGACAGTGGCGAGTTTGAGTGCAGGACAGAAGGGGTCT
  CGGCCTTCTTCGGCGTCACTGTCCAAGATCCTCCCGTGCACATCGTGGACCCCCGAGAACATGTGTTCGT
  GCATGCCATAACTTCCGAGTGTGTCATGCTGGCCTGTGAGGTGGACCGAGAGGACGCCCCTGTGCGTTGG
  TACAAGGACGGGCAGGAGGTGGAGGAGAGTGACTTCGTGGTGCTGGAGAATGAGGGGCCCCATCGCCGCC
  TGGTGCTGCCCGCCACCCAGCCCTCAGACGGGGGCGAGTTTCAGTGCGTCGCTGGAGATGAGTGTGCCTA
  CTTCACTGTCACCATCACAGACGTCTCCTCGTGGATCGTGTATCCCAGCGGCAAGGTGTATGTGGCAGCC
  GTGCGCCTGGAGCGTGTGGTGCTGACCTGTGAGCTATGCCGGCCCTGGGCAGAGGTGCGCTGGACCAAGG
  ATGGAGAGGAGGTGGTGGAGAGCCCCGCGCTGCTCCTGCAGAAGGAAGACACTGTCCGCCGCCTGGTGCT
  GCCCGCTGTCCAGCTCGAGGACTCCGGCGAGTACTTGTGTGAAATTGACGATGAGTCGGCCTCCTTCACT
  GTCACCGTCACAGAGTCTTACCAAAGTCAGGACAGTTCAAATAACAATCCGGAGTTATGCGTCCTCTTGA
  AAAAGCCGAAGACCCGGCGGCTCTGGTCCCGCTTCCCCCCATGGCGACGAACAGCTGGCACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230623 representing NM_001173408
  Red=Cloning site Green=Tags(s)
  
  MKASSGDQGSPPCFLRFPRPVRVVSGAEAELKCVVLGEPPPVVVWEKGGQQLAASERLSFPADGAEHGLL
  LTAALPTDAGVYVCRARNAAGEAYAAAAVTVLEPPASDPELQPAERPLPSPGSGEGAPVFLTGPRSQWVL
  RGAEVVLTCRAGGLPEPTLYWEKDGMALDEVWDSSHFALQPGRAEDGPGASLALRILAARLPDSGVYVCH
  ARNAHGHAQAGALLQVHQPPESPPADPDEAPAPVVEPLKCAPKTFWVNEGKHAKFRCYVMGKPEPEIEWH
  WEGRPLLPDRRRLMYRDRDGGFVLKVLYCQAKDRGLYVCAARNSAGQTLSAVQLHVKEPRLRFTRPLQDV
  EGREHGIAVLECKVPNSRIPTAWFREDQRLLPCRKYEQIEEGTVRRLIIHRLKADDDGIYLCEMRGRVRT
  VANVTVKGPILKRLPRKLDVLEGENAVLLVETLEAGVEGRWSRDGEELPVICQSSSGHMHALVLPGVTRE
  DAGEVTFSLGNSRTTTLLRVKCVKHSPPGPPILAEMFKGHKNTVLLTWKPPEPAPETPFIYRLERQEVGS
  EDWIQCFSIEKAGAVEVPGDCVPSEGDYRFRICTVSGHGRSPHVVFHGSAHLVPTARLVAGLEDVQVYDG
  EDAVFSLDLSTIIQGTWFLNGEELKSNEPEGQVEPGALRYRIEQKGLQHRLILHAVKHQDSGALVGFSCP
  GVQDSAALTIQESPVHILSPQDRVSLTFTTSERVVLTCELSRVDFPATWYKDGQKVEESELLVVKMDGRK
  HRLILPEAKVQDSGEFECRTEGVSAFFGVTVQDPPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRW
  YKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTITDVSSWIVYPSGKVYVAA
  VRLERVVLTCELCRPWAEVRWTKDGEEVVESPALLLQKEDTVRRLVLPAVQLEDSGEYLCEIDDESASFT
  VTVTESYQSQDSSNNNPELCVLLKKPKTRRLWSRFPPWRRTAGTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173408
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001173408.1, NP_001166879.1
• RefSeq ORF: 3078
• Locus ID: 23363
• MW: 113.7 kDa
• Gene Summary: Cytoskeletal adaptor proteins function in linking the internal cytoskeleton of cells to the cell membrane. This gene encodes a cytoskeletal adaptor protein, which is a member of the Unc-89/obscurin family. The protein contains multiple N- and C-terminal immunoglobulin (Ig)-like domains and a central fibronectin type 3 domain. Mutations in this gene cause 3M syndrome type 2. Alternatively spliced transcript variants encoding different isoforms have been found in this gene. [provided by RefSeq, Mar 2010]