IQSEC3 (NM_001170738) Human Tagged ORF Clone

CAT#: RC230670

IQSEC3 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 3 (IQSEC3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY433669) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230670 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IQSEC3
• Synonyms: KIAA1110; MGC30156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230670 representing NM_001170738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCCTGCTGGAGAATCCGGTGCGCGCCGTGCTCTACCTCAAGGAGCTCACGGCCATCGTGCAGA
  ACCAGCAGAGCCTCATCCACACCCAGCGAGAGCGTATCGACGAGCTGGAGCGGCGGCTGGACGAGCTGAG
  CGCTGAGAACCGCAGCCTGTGGGAGCACCAGCAGCTGCTGCAAGCCCAGCCTCCGCCCGGGCTCGTCCCC
  CCGTCATCGGCCCCGCTGCCGGCCGCTCCGGCCACCGCTCCTGCCGCCGCCGCCAGGGCCCAGGAACCTC
  TCCAGGACCAGGGACAGCGCTCAGCAGCCGCGCCGCATCCCGCGCCCGATCGGCCGCCGCGTCAGCACCA
  CGGACAGCTCCTGGAGCAGCCCCAGCGGGGCCCTGGCAGCAGGGCTCACACACCCCAGTCGCCCCACAAG
  CATCTGGGGACACAAGGGGCCGTGACTGACAAGGAGAAGGAGCGTCCCCCGAGTTGCTGCGCTGCTGCCG
  GAGCCCTCCTTCAGCACAAATCCCCCTCCGCCCTCGGCAAGGGCGTCCTGAGCAGGAGACCTGAGAATGA
  GACCGTGCTGCACCAGTTCTGCTGCCCAGCCGCCGACGCCTGCTCCGACCTGGCCTCCCAAAGTGATGGC
  TCCTGCACCCAGGCCGGTGGGGGCATGGAGGACTCCGTGGTGGCAGCGGCGGCGGTGGCAGCCGGCAGAC
  CCAGTGCCCATGCCCCGAAGGCTCAAGCCCAGGAGCTGCAGGAGGAGGAGGAGCGGCCGGGGGCAGGGGC
  TGCCTCCCCAAGGGCTGGCCCCCAGCACAAGGCCTCCCCCGGCCGGCAGCAGCCTGCCCTGGCGACGGCG
  CTGTGCCCCCACGCCCCTGCCGCCTCCGATTACGAACTCTCCCTTGACCTAAAGAATAAACAGATTGAAA
  TGCTAGAACATAAGTACGGCGGTCACCTGGTGTCCCGGCGCGCCGCTTGCACCATCCAAACCGCCTTCCG
  CCAATACCAGCTCAGCAAGAACTTCGAGAAAATCCGCAACTCGCTTCTGGAGAGCCGCCTGCCACGGCGG
  ATCTCCCTGCGCAAGGTGCGGTCACCCACGGCCGAGAGCCTGGCGGCCGAGAAAGCGCTCATGGAGGGCT
  ACGGCCTCGTGGGGCTGCCGCTGGTGCGCTCGCCCTCCCTGCCGCCCACCTTCGCAGGCACCCTCACCGA
  GCTGGAGGACTCCTTCACCGAGCAGGTGCAATCCCTGGCCAAGTCCATCGACGACGCGCTCAGCACGTGG
  AGCCTCAAGACCATGTGCTCCCTGCGGGAGAGTGGCGCTTACCAGCTCCACCAGGCCCTGCAGGCGGCCG
  CGGGGCCCCCAGGCCTGGAGGCCGAGGGGCGGGCGCCGGAGAGCGCGGGCCCCGGGCCCGGGGATGACGC
  CGCGGAGACCCCCGGCCTGCCCCCGGCCCACAGCGGGACCCTCATGATGGCTTTCCGGGACGTCACGGTG
  CAGATCGCCAACCAGAACATATCCGTCTCCTCCTCCACGGCTCTGTCGGTGGCCAACTGCCTGGGCGCTC
  AGACGGTCCAGGCCCCCGCAGAGCCCGCGGCGGGCAAGGCCGAGCAGGGCGAGACCTCTGGGCGGGAGGC
  CCCGGAAGCCCCCGCCGTGGGCCGGGAGGACGCGTCAGCCGAGGACTCATGCGCAGAGGCTGCGGCTAGT
  GGGGCGGCGGATGGGGCCACAGCCCCCAAAACAGAGGAGGAAGAGGAGGAGGAGGAGACGGCGGAGGTGG
  GGAGAGGGGCCGAGGCCGAGGCAGGCGACTTGGAGCAGCTGAGCAGCAGCAGCACGTCCACCAAGTCCGC
  CAAGTCAGGCTCGGAGGCGTCGGCCTCCGCCTCCAAGGACGCCCTGCAGGCCATGATCCTGAGCCTGCCG
  CGCTACCACTGCGAGAACCCAGCCAGCTGCAAGTCGCCCACGCTCTCCACCGACACCCTGCGCAAGCGGC
  TCTACCGCATCGGCCTCAACCTCTTCAACATAAACCCCGACAAGGGCATCCAGTTCCTGATCTCACGCGG
  CTTCATCCCGGACACCCCCATCGGTGTGGCCCATTTCCTCCTCCAGCGAAAGGGCCTCAGCCGCCAGATG
  ATTGGAGAGTTCCTGGGCAACAGCAAGAAGCAGTTCAACCGCGACGTGCTGGACTGCGTGGTGGACGAGA
  TGGACTTCTCCAGCATGGAGCTGGACGAGGCCCTGCGCAAGTTCCAGGCACACATCCGTGTGCAGGGGGA
  GGCTCAGAAGGTGGAGCGGCTCATTGAGGCCTTCAGCCAGCGCTACTGCATGTGCAACCCCGAAGTGGTT
  CAGCAGTTCCACAACCCCGACACCATCTTCATCCTCGCCTTCGCCATCATCCTCCTCAACACCGACATGT
  ACAGCCCCAACATCAAGCCTGACCGGAAGATGATGCTGGAGGACTTCATCCGAAACCTTCGAGGTGTGGA
  CGATGGCGCTGACATCCCCAGGGAGCTGGTGGTAGGCATCTATGAGAGGATACAGCAGAAGGAGCTCAAG
  TCCAATGAGGACCACGTCACGTACGTCACCAAGGTGGAGAAGTCCATTGTGGGCATGAAGACAGTGCTGT
  CCGTGCCCCACCGCCGCCTGGTGTGCTGCAGCCGGCTCTTCGAGGTGACGGATGTGAACAAGCTGCAGAA
  GCAGGCAGCGCATCAGAGGGAGGTGTTCCTCTTCAATGACCTGCTGGTGATTCTCAAACTTTGCCCGAAG
  AAGAAGAGCTCCTCCACGTACACCTTTTGCAAGTCAGTTGGCCTGCTGGGCATGCAGTTCCAGCTCTTTG
  AGAACGAGTATTACTCTCATGGCATCACACTGGTGACCCCGCTCTCGGGCTCCGAGAAGAAGCAGGTGCT
  GCATTTCTGTGCCCTGGGCTCGGACGAGATGCAGAAGTTCGTGGAGGACCTGAAGGAGTCCATTGCTGAG
  GTGACGGAGCTGGAGCAGATCCGAATAGAGTGGGAGCTGGAGAAGCAGCAGGGAACAAAGACACTCTCCT
  TCAAGCCCTGCGGAGCCCAGGGGGACCCACAGTCAAAGCAAGGATCGCCGACAGCCAAAAGGGAAGCCGC
  GCTCAGGGAGAGGCCGGCGGAGAGCACGGTGGAGGTGTCAATTCACAACAGGCTTCAAACGTCCCAGCAC
  AACTCCGGGCTGGGGGCCGAGAGGGGAGCGCCGGTGCCGCCGCCAGACCTGCAGCCTAGCCCCCCGAGAC
  AGCAGACCCCACCACTGCCGCCGCCGCCACCCACGCCCCCGGGCACCCTGGTGCAGTGCCAGCAAATTGT
  CAAGGTCATTGTCCTGGACAAGCCCTGCCTGGCCCGCATGGAGCCCCTGCTGAGCCAGGCTCTCTCCTGC
  TACACCTCGTCGTCCTCTGACTCCTGCGGCTCCACACCCCTGGGCGGTCCCGGCTCTCCGGTCAAGGTCA
  CCCACCAGCCTCCGCTGCCCCCGCCCCCACCCCCCTACAACCACCCTCACCAGTTCTGTCCCCCAGGCTC
  CCTGCTGCACGGGCACCGCTACTCCAGTGGCTCAAGGAGCCTGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230670 representing NM_001170738
  Red=Cloning site Green=Tags(s)
  
  MESLLENPVRAVLYLKELTAIVQNQQSLIHTQRERIDELERRLDELSAENRSLWEHQQLLQAQPPPGLVP
  PSSAPLPAAPATAPAAAARAQEPLQDQGQRSAAAPHPAPDRPPRQHHGQLLEQPQRGPGSRAHTPQSPHK
  HLGTQGAVTDKEKERPPSCCAAAGALLQHKSPSALGKGVLSRRPENETVLHQFCCPAADACSDLASQSDG
  SCTQAGGGMEDSVVAAAAVAAGRPSAHAPKAQAQELQEEEERPGAGAASPRAGPQHKASPGRQQPALATA
  LCPHAPAASDYELSLDLKNKQIEMLEHKYGGHLVSRRAACTIQTAFRQYQLSKNFEKIRNSLLESRLPRR
  ISLRKVRSPTAESLAAEKALMEGYGLVGLPLVRSPSLPPTFAGTLTELEDSFTEQVQSLAKSIDDALSTW
  SLKTMCSLRESGAYQLHQALQAAAGPPGLEAEGRAPESAGPGPGDDAAETPGLPPAHSGTLMMAFRDVTV
  QIANQNISVSSSTALSVANCLGAQTVQAPAEPAAGKAEQGETSGREAPEAPAVGREDASAEDSCAEAAAS
  GAADGATAPKTEEEEEEEETAEVGRGAEAEAGDLEQLSSSSTSTKSAKSGSEASASASKDALQAMILSLP
  RYHCENPASCKSPTLSTDTLRKRLYRIGLNLFNINPDKGIQFLISRGFIPDTPIGVAHFLLQRKGLSRQM
  IGEFLGNSKKQFNRDVLDCVVDEMDFSSMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCMCNPEVV
  QQFHNPDTIFILAFAIILLNTDMYSPNIKPDRKMMLEDFIRNLRGVDDGADIPRELVVGIYERIQQKELK
  SNEDHVTYVTKVEKSIVGMKTVLSVPHRRLVCCSRLFEVTDVNKLQKQAAHQREVFLFNDLLVILKLCPK
  KKSSSTYTFCKSVGLLGMQFQLFENEYYSHGITLVTPLSGSEKKQVLHFCALGSDEMQKFVEDLKESIAE
  VTELEQIRIEWELEKQQGTKTLSFKPCGAQGDPQSKQGSPTAKREAALRERPAESTVEVSIHNRLQTSQH
  NSGLGAERGAPVPPPDLQPSPPRQQTPPLPPPPPTPPGTLVQCQQIVKVIVLDKPCLARMEPLLSQALSC
  YTSSSSDSCGSTPLGGPGSPVKVTHQPPLPPPPPPYNHPHQFCPPGSLLHGHRYSSGSRSLV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170738
• ORF Size: 3546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170738.1, NP_001164209.1
• RefSeq ORF: 3549
• Locus ID: 440073
• Protein Pathways: Endocytosis
• MW: 128.1 kDa