PAN2 (NM_001166279) Human Tagged ORF Clone

CAT#: RC230675

PAN2 (Myc-DDK-tagged)-Human PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAN2
• Synonyms: USP52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230675 representing NM_001166279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTGAGGGTCTGGACCCTGGACTGGCAGAATATGCCCCAGCCATGCATTCTGCCCTGGACCCTG
  TCTTGGATGCCCACCTGAACCCAAGTCTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCCTTGGA
  GGCTCTTCCCGTCCAGGAATCAGTGCACATAATGGAAGGTGTCTACTCTGAATTGCACAGCGTGGTGGCT
  GAAGTGGGTGTACCTGTTTCCGTCTCCCACTTTGACTTGCACGAGGAGATGCTGTGGGTGGGGAGCCACG
  GGGGCCATGCCACTTCATTTTTTGGCCCAGCCTTGGAGCGCTACTCATCCTTTCAAGTCAATGGCAGTGA
  TGATATTCGGCAGATCCAGAGCCTGGAGAATGGTATCCTTTTTCTCACCAAGAACAACCTCAAGTATATG
  GCCCGTGGGGGCCTCATTATATTTGATTACCTGCTGGATGAGAATGAGGATATGCACAGTCTCCTACTGA
  CTGACAGCAGCACTCTACTCGTTGGTGGGCTGCAGAATCACATACTAGAGATTGATCTTAACACTGTCCA
  GGAGACTCAGAAGTATGCAGTAGAGACGCCTGGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTC
  TGCGGCCACACGTCTGGCAAGGTTTCCCTGAGAGACCTCCGTACTTTTAAGGTGGAACATGAGTTTGATG
  CCTTCTCAGGAAGTCTGTCAGACTTTGATGTGCATGGCAACCTGCTAGCTGCCTGTGGCTTCTCCAGCCG
  CCTCACTGGCCTGGCCTGCGACCGTTTCCTCAAGGTGTATGATTTGCGCATGATGCGTGCCATCACACCA
  CTTCAAGTACATGTGGATCCTGCCTTCTTGCGCTTCATTCCTACATATACTTCTCGTCTTGCTATCATCT
  CTCAGTCAGGGCAGTGCCAATTCTGTGAACCCACAGGCCTGGCCAACCCAGCCGATATCTTTCATGTGAA
  TCCTGTGGGGCCTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCTCTGGCCTTTGGGGATTCT
  GAGGGCTGTGTGCACCTCTGGACTGATTCCCCGGAGCCTTCCTTCAACCCCTACTCCCGTGAGACTGAGT
  TTGCTTTGCCGTGTCTCGTGGACTCACTGCCTCCTCTGGACTGGAGCCAGGACCTGCTGCCTCTTTCCCT
  CATCCCTGTCCCACTCACCACTGACACACTTCTCTCTGATTGGCCTGCTGCCAACTCTGCTCCAGCTCCC
  AGGCGAGCACCACCCGTGGATGCAGAGATTCTGCGCACCATGAAGAAGGTGGGCTTCATTGGCTATGCGC
  CCAATCCCCGCACCAGGCTGCGCAATCAGATACCCTACAGACTCAAGGAGTCAGACAGTGAATTTGACAG
  CTTCAGCCAGGTCACTGAGTCACCAGTAGGACGAGAAGAGGAACCACATCTCCACATGGTTTCTAAGAAA
  TACCGCAAGGTGACCATCAAATATTCCAAGCTAGGGCTGGAGGACTTTGACTTCAAACACTACAATAAGA
  CCTTGTTTGCTGGATTAGAGCCCCACATTCCCAACGCCTACTGTAACTGCATGATCCAGGTGCTCTATTT
  CCTGGAGCCTGTACGCTGTCTAATTCAAAACCACCTTTGCCAGAAGGAGTTCTGTCTGGCATGTGAGCTG
  GGCTTCCTGTTTCACATGTTGGACCTCTCTCGTGGTGACCCTTGCCAGGGCAATAATTTTCTTCGGGCAT
  TCCGTACTATTCCTGAGGCCTCAGCCCTCGGTCTAATCCTGGCTGACTCAGATGAGGCCTCAGGCAAGGG
  CAATCTGGCCAGGCTCATTCAGAGGTGGAATCGCTTCATTCTCACTCAACTGCATCAAGATATGCAGGAG
  CTGGAAATACCACAGGCTTATCGAGGTGCTGGAGGCAGCTTTTGCTCATCGGGGGACTCTGTTATTGGGC
  AGCTCTTCAGCTGTGAGATGGAGAACTGCAGCCTCTGCCGCTGTGGCAGTGAGACCGTGCGAGCCTCATC
  CACTCTGCTTTTCACACTCTCCTACCCTGATGGTAGCAAAAGTGATAAAACTGGGAAGAACTATGACTTT
  GCTCAGGTGCTGAAGCGAAGCATCTGCCTGGACCAGAATACACAGGCCTGGTGTGACACCTGTGAAAAGT
  ACCAGCCCACGATTCAGACCCGCAACATCCGCCATCTGCCAGATATTCTTGTCATCAATTGTGAGGTGAA
  CAGCTCAAAAGAGGCTGATTTCTGGAGAATGCAGGCTGAGGTTGCCTTCAAGATGGCAGTAAAGAAACAC
  GGTGGGGAAATCTCCAAGAACAAGGAATTTGCTTTGGCTGATTGGAAGGAACTAGGGAGTCCAGAGGGTG
  TGCTGGTGTGTCCCTCCATTGAGGAGTTGAAGAACGTCTGGCTTCCTTTCTCCATTCGCATGAAGATGAC
  CAAAAACAAAGGGCTGGATGTTTGCAATTGGACTGATGGGGATGAGATGCAGTGGGGCCCAGCCAGGGCA
  GAGGAGGAGCATGGTGTCTATGTGTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGCACAG
  GGGGCAGCCTGGTGGCTCACATCAAAGTTGGAGAGACCTACCACCAGCGCAAGGAGGGCGTTACTCACCA
  GCAGTGGTATCTGTTCAATGACTTTCTTATTGAACCTATTGATAAGCATGAAGCTGTGCAGTTTGACATG
  AATTGGAAAGTACCTGCAATCCTTTATTATGTCAAACGGAATCTCAATTCCAGATACAACCTGAACATCA
  AGAACCCTATTGAGGCAAGTGTCTTGCTGGCTGAAGCCTCGCTGGCACGGAAGCAGCGGAAAACACATAC
  TACCTTTATTCCACTGATGCTGAATGAGATGCCACAGATTGGGGACCTGGTGGGTCTGGATGCTGAGTTT
  GTCACCCTTAATGAGGAGGAAGCAGAGTTACGCAGTGATGGTACCAAGTCTACCATTAAACCAAGCCAGA
  TGTCAGTAGCCAGGATTACCTGTGTTCGGGGCCAGGGACCCAATGAGGGTATCCCCTTCATTGATGACTA
  CATCTCTACCCAGGAGCAGGTGGTGGATTACTTGACTCAATACTCGGGTATAAAGCCTGGTGACCTCGAT
  GCCAAAATTTCCTCCAAGCACCTAACAACTCTCAAGTCTACCTACTTAAAGCTTCGTTTTCTCATTGACA
  TTGGAGTCAAGTTTGTGGGTCATGGCCTGCAGAAGGACTTCCGGGTCATCAACCTGATGGTGCCCAAGGA
  CCAAGTCCTTGACACTGTCTACCTGTTCCATATGCCCCGAAAACGAATGATTTCCCTGCGATTCCTTGCT
  TGGTACTTTCTGGACCTGAAGATTCAAGGGGAAACCCATGACAGTATTGAGGATGCCCGCACAGCCCTTC
  AGCTGTACCGAAAGTATCTGGAGCTAAGCAAAAATGGCACTGAGCCTGAGTCTTTCCACAAGGTGCTCAA
  GGGTCTTTATGAGAAGGGCAGAAAGATGGACTGGAAGGTGCCTGAGCCTGAGGGCCAAACAAGTCCCAAG
  AATGCAGCTGTCTTCTCCTCAGTGCTGGCGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230675 representing NM_001166279
  Red=Cloning site Green=Tags(s)
  
  MNFEGLDPGLAEYAPAMHSALDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA
  EVGVPVSVSHFDLHEEMLWVGSHGGHATSFFGPALERYSSFQVNGSDDIRQIQSLENGILFLTKNNLKYM
  ARGGLIIFDYLLDENEDMHSLLLTDSSTLLVGGLQNHILEIDLNTVQETQKYAVETPGVTIMRQTNRFFF
  CGHTSGKVSLRDLRTFKVEHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITP
  LQVHVDPAFLRFIPTYTSRLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDS
  EGCVHLWTDSPEPSFNPYSRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDTLLSDWPAANSAPAP
  RRAPPVDAEILRTMKKVGFIGYAPNPRTRLRNQIPYRLKESDSEFDSFSQVTESPVGREEEPHLHMVSKK
  YRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACEL
  GFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQE
  LEIPQAYRGAGGSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDGSKSDKTGKNYDF
  AQVLKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKH
  GGEISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARA
  EEEHGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDM
  NWKVPAILYYVKRNLNSRYNLNIKNPIEASVLLAEASLARKQRKTHTTFIPLMLNEMPQIGDLVGLDAEF
  VTLNEEEAELRSDGTKSTIKPSQMSVARITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLD
  AKISSKHLTTLKSTYLKLRFLIDIGVKFVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLA
  WYFLDLKIQGETHDSIEDARTALQLYRKYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPEGQTSPK
  NAAVFSSVLAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166279
• ORF Size: 3603 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166279.1, NP_001159751.1
• RefSeq Size: 5298
• RefSeq ORF: 3606
• Locus ID: 9924
• Protein Families: Protease
• MW: 135.3 kDa
• Gene Summary: This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]