Cullin 7 (CUL7) (NM_001168370) Human Tagged ORF Clone

CAT#: RC230735

CUL7 (Myc-DDK-tagged)-Human cullin 7 (CUL7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CUL7
• Synonyms: 3M1; CUL-7; dJ20C7.5; KIAA0076
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230735 representing NM_001168370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGTGGCTTTTGGCTGGCGGAGCCGCTGGCAGGGACCGGGCCCCACCCTGCCCCAGTGGCTGCCG
  ACTCACGCGGGTGCAGCTCTGTTCCGCGGAGGCACGCCCCCTCCCGTCTCTCCGTCTCGACCCCCTCGCG
  GGGCCCAGGTGCCAGGATGGTGGGAGAACTCCGCTACAGGGAATTCAGGGTGCCCCTGGGGCCCGGCTTA
  CATGCCTATCCTGATGAGCTGATCCGCCAGCGCGTGGGCCATGATGGGCATCCTGAGTACCAGATCCGTT
  GGCTCATCCTGCGGCGTGGCGATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAGCACAT
  CCTGCTGTGGATGTCCAAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGCCAGGTC
  ATCGGGCCCTCCCAGGAGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAGGAGATGGAAA
  CCGACGTGAAGTCCCTCATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGTGTGGGCACTATCCCTCCTGC
  TCCTCTACTTCACACTGTCCACGTGCTCAGCGCCTATGCCAGCATTGAGCCCCTCACTGGAGTATTCAAG
  GACCCAAGGGTCCTGGACTTGCTCATGCACATGTTGAGTAGTCCTGATTATCAGATTCGCTGGAGTGCAG
  GCCGGATGATACAAGCCCTGTCCTCCCATGACGCTGGTGAGGGGCAGTGTGGGGAGGAAGGGAAAGCAGG
  AGAAGGGCTGGGTCGGCTCAGGGACTCACAGGACACTGTGGCAGGAGCCTCTGATCTCATCAGGACCCGG
  ACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAGAAACACCTGGATTTTGACAGCCGCTGTG
  CTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACCCCATGTCTTTCGAGGGCATTCAGCTACC
  ACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGGTATTTGCATGTCACCTCGCTCCTGGATCAG
  CTGAACGACAGTGCTGCGGAGCCAGGAGCCCAGAACACCTCTGCTCCTGAGGAGTTGAGTGGGGAGAGGG
  GTCAACTGGAGCTGGAGTTCAGTATGGCCATGGGCACCCTGATCTCGGAGCTGGTGCAAGCCATGCGCTG
  GGACCAGGCCTCAGACAGACCAAGGAGCTCAGCACGGTCCCCCGGTTCCATCTTCCAGCCTCAGCTGGCA
  GATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCCTTCAGGAGGTCAAGACGTTTTCGCCCTC
  GTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGCGGGACACACTGCAGCCGGGGATGCGAGT
  GCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGATGAGGGCGAGTTTCGGCAGAGCAACAACGGT
  GTGCCTCCTGTGCAGGTATTTTGGGAGTCAACAGGCCGCACCTATTGGGTGCACTGGCACATGCTGGAGA
  TCTTGGGCTTTGAGGAAGACATTGAGGACATGGTTGAGGCTGATGAGTACCAAGGGGCAGTGGCCAGTAG
  AGTCCTGGGTAGAGCCCTGCCTGCCTGGCGCTGGAGGCCCATGACAGAACTCTATGCTGTGCCTTATGTG
  CTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACCCTGGCTGAGTGGTGGGAACTCCTCTTCT
  TCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCCAGATCCTCCAGGAGAACCTAGATGGGGA
  GATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCCATAGAATTGGCCCAGGACTTGCTGCTGACT
  CTGCCACAGCGACTCAATGACAGTGCCCTCAGGGACCTGATCAACTGCCATGTCTACAAGAAGTATGGGC
  CTGAAGCCCTAGCAGGGAACCAAGCCTACCCATCCCTTCTAGAAGCCCAAGAAGATGTCCTCCTGCTAGA
  CGCGCAGGCCCAGGCTAAGGACTCAGAAGATGCAGCCAAAGTGGAAGCAAAAGAACCCCCATCTCAGAGT
  CCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGTCCAGCTGGGAAAATCCTCCTGGATCTAGAGC
  AAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTCAAGCCACTCCTGCTGCAGCTGCAGCGGCAGCC
  GCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACTCCGGAGACTAACAGGACCCTGCACCTGACTGTG
  CTGAGAATCCTGAAGCAGCTGGTGGACTTCCCCGAGGCACTGCTGCTCCCCTGGCACGAGGCCGTGGATG
  CCTGCATGGCCTGCCTGCGGTCCCCAAACACTGATCGAGAGGTGCTCCAGGAACTGATTTTCTTCCTGCA
  CCGCCTGACCTCAGTGAGCAGGGACTATGCCGTGGTGCTGAATCAGCTGGGAGCAAGAGACGCTATCTCC
  AAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAGCTGGCTCAGGAGCTGCGGGACATGGTGTTCAAGTGTG
  AGAAGCATGCCCACCTCTACCGCAAACTCATCACCAACATCCTGGGAGGCTGCATCCAGATGGTGCTGGG
  CCAGATCGAAGACCACAGACGAACCCACCAACCCATCAACATCCCTTTCTTTGATGTGTTCCTCAGATAC
  CTGTGCCAGGGCTCCAGTGTGGAAGTGAAGGAGGACAAGTGCTGGGAGAAGGTGGAGGTGTCCTCCAACC
  CGCACCGGGCCAGCAAGCTGACGGACCACAACCCCAAGACCTATTGGGAGTCCAACGGCAGCGCCGGCTC
  CCACTACATCACCCTGCACATGCGCCGGGGCATCCTCATCAGGCAACTGACTCTGCTTGTGGCTAGTGAG
  GACTCGAGTTACATGCCGGCCCGAGTGGTGGTGTGCGGGGGTGATAGCACTAGCTCTCTTCACACGGAAC
  TCAACTCGGTGAATGTGATGCCCTCTGCCAGCCGGGTGATCCTCCTGGAGAACCTGACCCGCTTCTGGCC
  CATCATCCAGATCCGCATAAAGCGCTGCCAGCAGGGTGGCATTGATACGCGCATTCGGGGGTTAGAGATC
  CTAGGCCCCAAGCCCACGTTCTGGCCAGTGTTCCGGGAGCAGCTCTGTCGTCACACACGCCTCTTCTACA
  TGGTTCGGGCACAGGCCTGGAGCCAGGACATGGCAGAGGACCGCAGGAGCCTCCTGCACCTGAGTTCTAG
  ACTCAACGGTGCTCTGCGCCAGGAGCAGAATTTTGCTGACCGCTTCCTCCCTGATGACGAGGCTGCCCAA
  GCTCTGGGCAAGACCTGCTGGGAGGCCCTGGTCAGCCCCGTGGTGCAGAACATCACCTCCCCTGATGAGG
  ATGGCATTAGCCCCCTGGGTTGGCTGCTGGACCAGTACCTGGAGTGTCAGGAAGCTGTCTTCAACCCCCA
  GAGCCGCGGCCCAGCTTTCTTCTCGCGGGTGCGCCGTCTCACTCACCTGCTGGTGCATGTCGAGCCCTGT
  GAGGCACCCCCTCCTGTGGTGGCCACTCCTCGGCCCAAAGGCAGAAACAGAAGCCACGACTGGAGCTCCT
  TGGCTACCCGGGGCCTTCCAAGCAGCATCATGAGAAACCTGACGCGCTGTTGGCGGGCCGTGGTGGAGAA
  GCAGGTGAACAATTTTCTGACCTCATCCTGGCGGGATGATGACTTTGTGCCACGCTACTGTGAGCACTTT
  AATATTCTGCAGAACTCAAGCTCTGAACTGTTTGGGCCTCGGGCAGCCTTCTTGCTGGCGCTGCAAAATG
  GCTGTGCGGGAGCCTTGCTGAAGCTCCCTTTTCTCAAAGCTGCCCACGTGAGTGAGCAGTTCGCCCGGCA
  CATTGACCAGCAGATCCAGGGCAGCCGGATCGGTGGAGCCCAGGAAATGGAGAGGCTGGCACAGCTGCAG
  CAATGCCTGCAAGCTGTCCTGATTTTCTCCGGCTTGGAGATAGCCACCACTTTTGAGCATTATTACCAGC
  ACTACATGGCGGACCGTCTCCTGGGCGTGGTCTCGAGCTGGCTGGAGGGGGCCGTGCTGGAGCAGATCGG
  TCCCTGCTTCCCCAACCGCCTCCCCCAGCAGATGTTGCAGAGCCTGAGCACCTCTAAGGAGCTGCAGCGC
  CAGTTCCACGTCTACCAGCTCCAGCAGCTGGATCAGGAACTCCTGAAGCTGGAGGATACAGAGAAGAAAA
  TACAGGTGGGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCGAGAAGGAAGAGGAAGCTGGGGCAGCAGC
  AGTGGTGGATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAGAATGAGGACCTCTACTATGAAGGGGCAATG
  CCAGAAGTGTCTGTGCTTGTCCTGTCCCGACACTCCTGGCCTGTTGCCTCAATCTGCCACACACTGAACC
  CCAGAACCTGCCTGCCCTCCTACCTGAGGGGCACTTTGAACAGATACTCCAACTTCTACAACAAGAGTCA
  GAGCCACCCTGCCCTTGAGCGAGGCTCACAGAGGCGACTGCAGTGGACGTGGCTGGGCTGGGCTGAGCTG
  CAGTTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATGTGGCTACTGCTGTATCTCAACGACCTGA
  AGGCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCTCCGCAGACATGCTCAATCAGGCGATTGG
  GCCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAGCAAAAGGATATACCAGGAGGGGTCCTCAAG
  ATTCGAGATGGCAGCAAGGAACCCAGGTCGAGATGGGACATTGTGCGGCTCATCCCACCTCAGACGTACC
  TGCAAGCTGAGGGTGAAGACGGCCAGAACTTGGAGAAGAGACGGAATCTTCTGAACTGCCTCATCGTCCG
  AATCCTCAAGGCCCATGGAGATGAGGGGCTGCACATTGACCAGCTTGTCTGTCTGGTGCTGGAGGCTTGG
  CAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTTGGTAAGGGGTCTGCATGCAGCAGCACTG
  ACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGCTGAGACGCCATGACGACCGGCCCCAGGT
  GCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCACACTGAGTCCCTGAACCCAGGCTCCTCAGGC
  CCCAACCCACCCCTCACCTTCCATACCCTACAGATTCGCTCCCGGGGTGTGCCCTATGCCTCCTGCACTG
  CCACCCAGAGCTTCTCTACCTTCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230735 representing NM_001168370
  Red=Cloning site Green=Tags(s)
  
  MSRGFWLAEPLAGTGPHPAPVAADSRGCSSVPRRHAPSRLSVSTPSRGPGARMVGELRYREFRVPLGPGL
  HAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQVDCKAEHILLWMSKDEIYANCHKMLGEDGQV
  IGPSQESAGEVGALDKSVLEEMETDVKSLIQRALRQLEECVGTIPPAPLLHTVHVLSAYASIEPLTGVFK
  DPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGEGQCGEEGKAGEGLGRLRDSQDTVAGASDLIRTR
  TQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQ
  LNDSAAEPGAQNTSAPEELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLA
  DVSPGLPAAQAQPSFRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEFRQSNNG
  VPPVQVFWESTGRTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRALPAWRWRPMTELYAVPYV
  LPEDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQILQENLDGEILDDEILAELAVPIELAQDLLLT
  LPQRLNDSALRDLINCHVYKKYGPEALAGNQAYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQS
  PNTPLQRLVEGYGPAGKILLDLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTV
  LRILKQLVDFPEALLLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAIS
  KALEKHLGKLELAQELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHQPINIPFFDVFLRY
  LCQGSSVEVKEDKCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYITLHMRRGILIRQLTLLVASE
  DSSYMPARVVVCGGDSTSSLHTELNSVNVMPSASRVILLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEI
  LGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQ
  ALGKTCWEALVSPVVQNITSPDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPC
  EAPPPVVATPRPKGRNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHF
  NILQNSSSELFGPRAAFLLALQNGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQ
  QCLQAVLIFSGLEIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQR
  QFHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEENEDLYYEGAM
  PEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAEL
  QFGNQTLHVSTVQMWLLLYLNDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLK
  IRDGSKEPRSRWDIVRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAW
  QKGPCPPRGLVSSLGKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSG
  PNPPLTFHTLQIRSRGVPYASCTATQSFSTFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168370
• ORF Size: 5346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168370.1, NP_001161842.1
• RefSeq ORF: 5349
• Locus ID: 9820
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 200.2 kDa
• Gene Summary: The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]