CACNA1C (NM_001167624) Human Tagged ORF Clone

CAT#: RC230745

CACNA1C (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant 22

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1C
• Synonyms: CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1; LQT8; TS; TS. LQT8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230745 representing NM_001167624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAATGAGAATACGAGGATGTACATTCCAGAGGAAAACCACCAAGGTTCCAACTATGGGAGCCCAC
  GCCCCGCCCATGCCAACATGAATGCCAATGCGGCAGCGGGGCTGGCCCCTGAGCACATCCCCACCCCGGG
  GGCTGCCCTGTCGTGGCAGGCGGCCATCGACGCAGCCCGGCAGGCTAAGCTGATGGGCAGCGCTGGCAAT
  GCGACCATCTCCACAGTCAGCTCCACGCAGCGGAAGCGGCAGCAATATGGGAAACCCAAGAAGCAGGGCA
  GCACCACGGCCACACGCCCGCCCCGAGCCCTGCTCTGCCTGACCCTGAAGAACCCCATCCGGAGGGCCTG
  CATCAGCATTGTCGAATGGAAACCATTTGAAATAATTATTTTACTGACTATTTTTGCCAATTGTGTGGCC
  TTAGCGATCTATATTCCCTTTCCAGAAGATGATTCCAACGCCACCAATTCCAACCTGGAACGAGTGGAAT
  ATCTCTTTCTCATAATTTTTACGGTGGAAGCGTTTTTAAAAGTAATCGCCTATGGACTCCTCTTTCACCC
  CAATGCCTACCTCCGCAACGGCTGGAACCTACTAGATTTTATAATTGTGGTTGTGGGGCTTTTTAGTGCA
  ATTTTAGAACAAGCAACCAAAGCAGATGGGGCAAACGCTCTCGGAGGGAAAGGGGCCGGATTTGATGTGA
  AGGCGCTGAGGGCCTTCCGCGTGCTGCGCCCCCTGCGGCTGGTGTCCGGAGTCCCAAGTCTCCAGGTGGT
  CCTGAATTCCATCATCAAGGCCATGGTCCCCCTGCTGCACATCGCCCTGCTTGTGCTGTTTGTCATCATC
  ATCTACGCCATCATCGGCTTGGAGCTCTTCATGGGGAAGATGCACAAGACCTGCTACAACCAGGAGGGCA
  TAGCAGATGTTCCAGCAGAAGATGACCCTTCCCCTTGTGCGCTGGAAACGGGCCACGGGCGGCAGTGCCA
  GAACGGCACGGTGTGCAAGCCCGGCTGGGATGGTCCCAAGCACGGCATCACCAACTTTGACAACTTTGCC
  TTCGCCATGCTCACGGTGTTCCAGTGCATCACCATGGAGGGCTGGACGGACGTGCTGTACTGGATGCAGG
  ACGCTATGGGCTATGAGTTACCCTGGGTGTATTTTGTCAGTCTGGTCATCTTTGGATCCTTTTTCGTTCT
  AAATCTGGTTCTCGGTGTGTTGAGCGGAGAGTTTTCCAAAGAGAGGGAGAAGGCCAAGGCCCGGGGAGAT
  TTCCAGAAGCTGCGGGAGAAGCAGCAGCTAGAAGAGGATCTCAAAGGCTACCTGGATTGGATCACTCAGG
  CCGAAGACATCGATCCTGAGAATGAGGACGAAGGCATGGATGAGGAGAAGCCCCGAAACATGAGCATGCC
  CACCAGTGAGACCGAGTCCGTCAACACCGAAAACGTGGCTGGAGGTGACATCGAGGGAGAAAACTGCGGG
  GCCAGGCTGGCCCACCGGATCTCCAAGTCAAAGTTCAGCCGCTACTGGCGCCGGTGGAATCGGTTCTGCA
  GAAGGAAGTGCCGCGCCGCAGTCAAGTCTAATGTCTTCTACTGGCTGGTGATTTTCCTGGTGTTCCTCAA
  CACGCTCACCATTGCCTCTGAGCACTACAACCAGCCCAACTGGCTCACAGAAGTCCAAGACACGGCAAAC
  AAGGCCCTGCTGGCCCTGTTCACGGCAGAGATGCTCCTGAAGATGTACAGCCTGGGCCTGCAGGCCTACT
  TCGTGTCCCTCTTCAACCGCTTTGACTGCTTCGTCGTGTGTGGCGGCATCCTGGAGACCATCCTGGTGGA
  GACCAAGATCATGTCCCCACTGGGCATCTCCGTGCTCAGATGCGTCCGGCTGCTGAGGATTTTCAAGATC
  ACGAGGTACTGGAACTCCTTGAGCAACCTGGTGGCATCCTTGCTGAACTCTGTGCGCTCCATCGCCTCCC
  TGCTCCTTCTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATGCAGCTCTTTGGAGGAAAGTT
  CAACTTTGATGAGATGCAGACCCGGAGGAGCACATTCGATAACTTCCCCCAGTCCCTCCTCACTGTGTTT
  CAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCATGGCTTATGGCGGCCCCTCTT
  TTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGAAACTATATCCTACTGAATGT
  GTTCTTGGCCATTGCTGTGGACAACCTGGCTGATGCTGAGAGCCTCACATCTGCCCAAAAGGAGGAGGAA
  GAGGAGAAGGAGAGAAAGAAGCTGGCCAGGACTGCCAGCCCAGAGAAGAAACAAGAGTTGGTGGAGAAGC
  CGGCAGTGGGGGAATCCAAGGAGGAGAAGATTGAGCTGAAATCCATCACGGCTGACGGAGAGTCTCCACC
  CGCCACCAAGATCAACATGGATGACCTCCAGCCCAATGAAAATGAGGATAAGAGCCCCTACCCCAACCCA
  GAAACTACAGGAGAAGAGGATGAGGAGGAGCCAGAGATGCCTGTCGGCCCTCGCCCACGACCACTCTCTG
  AGCTTCACCTTAAGGAAAAGGCAGTGCCCATGCCAGAAGCCAGCGCGTTTTTCATCTTCAGCTCTAACAA
  CAGGTTTCGCCTCCAGTGCCACCGCATTGTCAATGACACGATCTTCACCAACCTGATCCTCTTCTTCATT
  CTGCTCAGCAGCATTTCCCTGGCTGCTGAGGACCCGGTCCAGCACACCTCCTTCAGGAACCATATTCTGT
  TTTATTTTGATATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTGAAGATGACTGCTTATGGGGC
  TTTCTTGCACAAGGGTTCTTTCTGCCGGAACTACTTCAACATCCTGGACCTGCTGGTGGTCAGCGTGTCC
  CTCATCTCCTTTGGCATCCAGTCCAGTGCAATCAATGTCGTGAAGATCTTGCGAGTCCTGCGAGTACTCA
  GGCCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCATGTGGTTCAGTGTGTGTTTGTCGCCATCCG
  GACCATCGGGAACATCGTGATTGTCACCACCCTGCTGCAGTTCATGTTTGCCTGCATCGGGGTCCAGCTC
  TTCAAGGGAAAGCTGTACACCTGTTCAGACAGTTCCAAGCAGACAGAGGCGGAATGCAAGGGCAACTACA
  TCACGTACAAAGACGGGGAGGTTGACCACCCCATCATCCAACCCCGCAGCTGGGAGAACAGCAAGTTTGA
  CTTTGACAATGTTCTGGCAGCCATGATGGCCCTCTTCACCGTCTCCACCTTCGAAGGGTGGCCAGAGCTG
  CTGTACCGCTCCATCGACTCCCACACGGAAGACAAGGGCCCCATCTACAACTACCGTGTGGAGATCTCCA
  TCTTCTTCATCATCTACATCATCATCATCGCCTTCTTCATGATGAACATCTTCGTGGGCTTCGTCATCGT
  CACCTTTCAGGAGCAGGGGGAGCAGGAGTACAAGAACTGTGAGCTGGACAAGAACCAGCGACAGTGCGTG
  GAATACGCCCTCAAGGCCCGGCCCCTGCGGAGGTACATCCCCAAGAACCAGCACCAGTACAAAGTGTGGT
  ACGTGGTCAACTCCACCTACTTCGAGTACCTGATGTTCGTCCTCATCCTGCTCAACACCATCTGCCTGGC
  CATGCAGCACTACGGCCAGAGCTGCCTGTTCAAAATCGCCATGAACATCCTCAACATGCTCTTCACTGGC
  CTCTTCACCGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGGGTTACTTTAGTGATCCCTGGA
  ATGTTTTTGACTTCCTCATCGTAATTGGCAGCATAATTGACGTCATTCTCAGTGAGACTAATCCAGCTGA
  ACATACCCAATGCTCTCCCTCTATGAACGCAGAGGAAAACTCCCGCATCTCCATCACCTTCTTCCGCCTG
  TTCCGGGTCATGCGTCTGGTGAAGCTGCTGAGCCGTGGGGAGGGCATCCGGACGCTGCTGTGGACCTTCA
  TCAAGTCCTTCCAGGCCCTGCCCTATGTGGCCCTCCTGATCGTGATGCTGTTCTTCATCTACGCGGTGAT
  CGGGATGCAGGTGTTTGGGAAAATTGCCCTGAATGATACCACAGAGATCAACCGGAACAACAACTTTCAG
  ACCTTCCCCCAGGCCGTGCTGCTCCTCTTCAGGTGTGCCACCGGGGAGGCCTGGCAGGACATCATGCTGG
  CCTGCATGCCAGGCAAGAAGTGTGCCCCAGAGTCCGAGCCCAGCAACAGCACGGAGGGTGAAACACCCTG
  TGGTAGCAGCTTTGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTGCCTTCCTGATCATCAACCTC
  TTTGTAGCTGTCATCATGGACAACTTTGACTACCTGACAAGGGACTGGTCCATCCTTGGTCCCCACCACC
  TGGATGAGTTTAAAAGAATCTGGGCAGAGTATGACCCTGAAGCCAAGGGTCGTATCAAACACCTGGATGT
  GGTGACCCTCCTCCGGCGGATTCAGCCGCCACTAGGTTTTGGGAAGCTGTGCCCTCACCGCGTGGCTTGC
  AAACGCCTGGTCTCCATGAACATGCCTCTGAACAGCGACGGGACAGTCATGTTCAATGCCACCCTGTTTG
  CCCTGGTCAGGACGGCCCTGAGGATCAAAACAGAAGGGAACCTAGAACAAGCCAATGAGGAGCTGCGGGC
  GATCATCAAGAAGATCTGGAAGCGGACCAGCATGAAGCTGCTGGACCAGGTGGTGCCCCCTGCAGGTGAT
  GATGAGGTCACCGTTGGCAAGTTCTACGCCACGTTCCTGATCCAGGAGTACTTCCGGAAGTTCAAGAAGC
  GCAAAGAGCAGGGCCTTGTGGGCAAGCCCTCCCAGAGGAACGCGCTGTCTCTGCAGGCTGGCTTGCGCAC
  ACTGCATGACATCGGGCCTGAGATCCGACGGGCCATCTCTGGAGATCTCACCGCTGAGGAGGAGCTGGAC
  AAGGCCATGAAGGAGGCTGTGTCCGCTGCTTCTGAAGATGACATCTTCAGGAGGGCCGGTGGCCTGTTCG
  GCAACCACGTCAGCTACTACCAAAGCGACGGCCGGAGCGCCTTCCCCCAGACCTTCACCACTCAGCGCCC
  GCTGCACATCAACAAGGCGGGCAGCAGCCAGGGCGACACTGAGTCGCCATCCCACGAGAAGCTGGTGGAC
  TCCACCTTCACCCCGAGCAGCTACTCGTCCACCGGCTCCAACGCCAACATCAACAACGCCAACAACACCG
  CCCTGGGTCGCCTCCCTCGCCCCGCCGGCTACCCCAGCACGGTCAGCACTGTGGAGGGCCACGGGCCCCC
  CTTGTCCCCTGCCATCCGGGTGCAGGAGGTGGCGTGGAAGCTCAGCTCCAACAGGGAAAGGCACGTTCCG
  ATGTGTGAGGATCTGGAGCTCAGGAGGGATTCAGGCTCAGCAGGGACTCAGGCTCACTGCCTTCTGCTCA
  GGAAAGCAAACCCCTCTAGGTGCCACTCCCGGGAGAGCCAGGCAGCCATGGCGGGTCAGGAGGAGACGTC
  TCAGGATGAGACCTATGAAGTGAAGATGAACCATGACACGGAGGCCTGCAGTGAGCCCAGCCTGCTCTCC
  ACAGAGATGCTCTCCTACCAGGATGACGAAAATCGGCAACTGACGCTCCCAGAGGAGGACAAGAGGGACA
  TCCGGCAATCTCCGAAGAGGGGTTTCCTCCGCTCTGCCTCACTAGGTCGAAGGGCCTCCTTCCACCTGGA
  ATGTCTGAAGCGACAGAAGGACCGAGGGGGAGACATCTCTCAGAAGACAGTCCTGCCCTTGCATCTGGTT
  CATCATCAGGCATTGGCAGTGGCAGGCCTGAGCCCCCTCCTCCAGAGAAGCCATTCCCCTGCCTCATTCC
  CTAGGCCTTTTGCCACCCCACCAGCCACACCTGGCAGCCGAGGCTGGCCCCCACAGCCCGTCCCCACCCT
  GCGGCTTGAGGGGGTCGAGTCCAGTGAGAAACTCAACAGCAGCTTCCCATCCATCCACTGCGGCTCCTGG
  GCTGAGACCACCCCCGGTGGCGGGGGCAGCAGCGCCGCCCGGAGAGTCCGGCCCGTCTCCCTCATGGTGC
  CCAGCCAGGCTGGGGCCCCAGGGAGGCAGTTCCACGGCAGTGCCAGCAGCCTGGTGGAAGCGGTCTTGAT
  TTCAGAAGGACTGGGGCAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACCCAGGAGCTGGCCGAC
  GCCTGCGACATGACCATAGAGGAGATGGAGAGCGCGGCCGACAACATCCTCAGCGGGGGCGCCCCACAGA
  GCCCCAATGGCGCCCTCTTACCCTTTGTGAACTGCAGGGACGCGGGGCAGGACCGAGCCGGGGGCGAAGA
  GGACGCGGGCTGTGTGCGCGCGCGGGGTCGACCGAGTGAGGAGGAGCTCCAGGACAGCAGGGTCTACGTC
  AGCAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230745 representing NM_001167624
  Red=Cloning site Green=Tags(s)
  
  MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGN
  ATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVA
  LAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSA
  ILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVII
  IYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFA
  FAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGD
  FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCG
  ARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTAN
  KALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKI
  TRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVF
  QILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEE
  EEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNP
  ETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFI
  LLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVS
  LISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQL
  FKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPEL
  LYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCV
  EYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTG
  LFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRL
  FRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQ
  TFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINL
  FVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVAC
  KRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGD
  DEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELD
  KAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVD
  STFTPSSYSSTGSNANINNANNTALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRERHVP
  MCEDLELRRDSGSAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETYEVKMNHDTEACSEPSLLS
  TEMLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQKTVLPLHLV
  HHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGVESSEKLNSSFPSIHCGSW
  AETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELAD
  ACDMTIEEMESAADNILSGGAPQSPNGALLPFVNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYV
  SSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167624
• ORF Size: 6519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167624.1, NM_001167624.2, NP_001161096.1
• RefSeq ORF: 6522 bp
• Locus ID: 775
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), Long-term potentiation, MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction
• MW: 244.1 kDa
• Gene Summary: 'This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012]'