DOCK8 (NM_001190458) Human Tagged ORF Clone

CAT#: RC230900

DOCK8 (Myc-DDK-tagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DOCK8
• Synonyms: HEL-205; MRD2; ZIR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230900 representing NM_001190458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGG
  ACGTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGA
  CCCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA
  AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAGA
  CGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTGCT
  GTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGACAAG
  CGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGGAGGA
  CCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATACGTCC
  AGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAGTTCGAG
  ATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATCTCAGAAA
  ATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCAGTGGCCGC
  ATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTGGTAGTCAAG
  ATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATCAAAGAAAGTG
  ATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGCCAGCGTTTGGG
  GAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAGTGTCTCCACCCTTGAG
  AGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGGACATTGGCCCAAT
  CTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAACTTCAAAACCTCCAC
  TCTGAGCGTTAGCATCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGACTTATTCAAGTTTTTA
  GCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGCTTGCTAAGACTGGAGA
  TTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCCGTGAAACCCTTTCCTGA
  AAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTATATGTCCCTCACACTGTG
  TACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAACTAGCATCAGCCCGGAACA
  TTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATGCCGGTCATCTTTGGAAAGTC
  CAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCATAATAAGTCTCCTGACTTTTAT
  GAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCACCTCCTGTTCACCTTCTACCATA
  TCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGATATTCATGGCTGCCAATTCTCTT
  AAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTGGAAAAATTGCCACCCAACTACTCC
  ATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAGTGGGCTGAAGGACATAAGGGAGTAT
  TTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGACAACCACCTGGAGAAGTTCTTCACCCT
  CTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTGCTGGATCAGAAAATCAGCGAGATGGCG
  CTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTCAACTCCTCCCGCCTGGAGCCGCTCGTGCTCTTCC
  TGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAGCCCATGGTCATCGCTGGCCAGACAGCCAA
  CTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCCAACAGTCTGCACAACAGCAAGGACCTGAGC
  AAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTACGTGCACTACGTCTTCCGCCTGCCAGAGGTGC
  AAAGGGATGTGCCCAAGTCAGGCGCTCCCACTGCCCTCCTAGACCCTCGGAGCTACCACACGTATGGCCG
  CACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAGGCCCGGGTGATGAGCAGCAGTAACCCAGACCTC
  GCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAGAACATCATGTCTTCAAAGCATTTCCATGAGGAGC
  TTGCCCTTCAGATGGTGGTCAGCACTGGAATGGTGAGAGAAACAGTCTTCAAGTATGCCTGGTTCTTCTT
  TGAGCTTCTGGTGAAAAGCATGGCCCAGCACGTACATAACATGGACAAACGGGACAGTTTTCGGAGGACT
  CGTTTTTCTGACCGTTTCATGGATGACATAACTACTATTGTTAATGTGGTCACCTCGGAAATTGCAGCCC
  TTTTAGTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATGAACATCAGCCTGGCTTTCTTCTTGTA
  TGACCTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCATCAGACATTATTGCAGCCAGCTGTCA
  GCCAAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGAGTTCCTGAGAATCCTCTGTAGCCATG
  AGCATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACTGCTCCAACATCTCCTTGTCCTTCCAT
  ATCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGAAGATCGCCAGCATGTTCGATCTGACT
  TCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCACAGAACTGGCTGCTGCCCTGGATGCCG
  AAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCAATTCACAGCCTGCTAAGTTCTCACGA
  CCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCGCCGCCCTTTACCTACCTTTAGTTGGC
  ATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGCAGATACTCGCAGATACCGCACCAGTG
  GCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAATGTGGCTCTGGCCATAGCAGGGAATAA
  TTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCTATAAGCAGTACAACATGCTGAACGCG
  GACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAAAAATGCTGATCAGAGCCTCATTAGGA
  AGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTAGATCTACTTTTCATCTGTGTGTTATG
  TTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCCAAGTCCTGCAGAAGTCAAGGGATGTC
  AAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAGAGGGGAGATGATGCGCCGCCGGGCTC
  CAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGGAAGAAAGAGCAGACACATTGGCGGCA
  AGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAGAAGCCTTGATCAGTGGCAATCTGGCT
  ACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTATCCAGGCGAGCTCGGCTCTGGACTGTA
  AAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCTCTGAACTGTGATCAGAGTACCACCTA
  CCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGTTTGGAGACTTACTCTTCGAAGAGGAG
  GTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTGCAGCAGCAGCATGGATGTCACCCGGA
  GCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTTGGAGCCACCAGTAATTTTGCAAGAGT
  AAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAGCACCAGACTTTAATGAAGAGCACCTG
  AGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACACAGCCATGCAGATGACTCCTTTTCCCA
  CCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATATGACACAGTGAAAATGAGGGAATTTCA
  GGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCAAGAGTTACCAGGCATCTCCTGATCTG
  CGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAAGAAGTGCTACACGGAGGCTGCCATGT
  GCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATGCTGGAGGACCACAGCTACCTGCCCGT
  GGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGGAGTCTGTGGTCTCTGAGGACACCCTG
  TCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGAGAGTGGCCTGGTAGGCCTCCTGGAGC
  AGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTTAATGAGGTCTACAAGCTGGTCATCCC
  CATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACTCACAGCAAGCTGCAGAGAGCCTTCGAC
  AGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTACTTCCGAGTTGGTTTCTTTGGATCCAAAT
  TTGGGGATTTGGATGAACAGGAATTTGTCTACAAAGAGCCTGCAATTACCAAGCTTCCTGAGATCTCACA
  TAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAATTTGTGGAAGTGATTAAAGACTCCACTCCT
  GTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATACAGATCACTTTTGTGGAGCCCTACTTTGATG
  AGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAGAATTTCAACCTCCGGAGGTTCATGTACACCAC
  CCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTGCATGAGCAGTACAGAAGGAACACAGTCCTGACC
  ACTATGCACGCCTTCCCCTTCATCAAGACCAGGATCAGCGTCATCCAGAAGGAGGAGTTTGTTTTGACAC
  CGATTGAAGTTGCCATTGAAGACATGAAGAAGAAGACCCTGCAGTTAGCAGTTGCCATTAACCAGGAGCC
  GCCTGATGCAAAGATGCTTCAGATGGTGCTGCAAGGCTCTGTGGGAGCTACTGTAAATCAGGGACCACTG
  GAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCTGCTGATCCAAAACTCTATCGACATCACAACAAGTTGA
  GGTTATGCTTTAAGGAATTCATCATGAGATGTGGTGAAGCTGTAGAGAAAAACAAGCGTCTCATCACGGC
  AGACCAGAGGGAATATCAGCAGGAACTCAAAAAGAACTATAACAAGCTAAAAGAGAACCTCAGGCCAATG
  ATCGAGCGGAAAATTCCAGAACTGTACAAGCCAATATTCAGAGTTGAGAGTCAAAAGAGGGACTCCTTCC
  ACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230900 representing NM_001190458
  Red=Cloning site Green=Tags(s)
  
  MTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQG
  SPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDK
  RLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFE
  IEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVK
  IEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFSVSTLE
  REVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSIFFKQEGDRLSDEDLFKFL
  ADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTV
  YRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFY
  EEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYS
  MHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMA
  LEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLS
  KDQHGRNCLLASYVHYVFRLPEVQRDVPKSGAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDL
  AGTHSAADEEVKNIMSSKHFHEELALQMVVSTGMVRETVFKYAWFFFELLVKSMAQHVHNMDKRDSFRRT
  RFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKMNISLAFFLYDLLSLMDRGFVFNLIRHYCSQLS
  AKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADTAPTSPCPSISSQNSSSCSSFQDQKIASMFDLT
  SEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSAIHSLLSSHDLDPRCVKPEVKVKIAALYLPLVG
  IILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQNVALAIAGNNFNLKTSGIVLSSLPYKQYNMLNA
  DTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRILDLLFICVLCFEYKGKQSSDKVSTQVLQKSRDV
  KARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRWKKEQTHWRQANEKLDKTKAELDQEALISGNLA
  TEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLNCDQSTTYLTHCFATLRALIAKFGDLLFEEE
  VEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMSLASLVGRAPDFNEEHL
  RRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDL
  RLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESVVSEDTL
  SPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHREFRKLTLTHSKLQRAFD
  SIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPAITKLPEISHRLEAFYGQCFGAEFVEVIKDSTP
  VDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQYRRNTVLT
  TMHAFPFIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLAVAINQEPPDAKMLQMVLQGSVGATVNQGPL
  EVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPM
  IERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190458
• ORF Size: 5997 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190458.1, NP_001177387.1
• RefSeq ORF: 6000
• Locus ID: 81704
• MW: 227.9 kDa
• Gene Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]