PRSS55 (NM_001197020) Human Tagged ORF Clone

CAT#: RC231117

PRSS55 (Myc-DDK-tagged)-Human protease, serine, 55 (PRSS55), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY434116) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231117 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRSS55 protein (Cat# TP331117). The protein was produced from HEK293T cells transfected with PRSS55 cDNA clone (Cat# RC231117) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRSS55
• Synonyms: CT153; T-SP1; TSP1; UNQ9391
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231117 representing NM_001197020
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGTTCTCAGTGTTGCTGCTCCTGTCCCTGGTCACGGGAACTCAGCTCGGTCCACGGACTCCTC
  TCCCAGAGGCTGGAGTGGCTATCCTAGGCAGGGCTAGGGGAGCCCACCGCCCTCAGCCCCCTCATCCCCC
  CAGCCCAGTCAGTGAATGTGGTGACAGATCTATTTTCGAGGGAAGAACTCGGTATTCCAGAATCACAGGG
  GGGATGGAGGCGGAGGTGGGTGAGTTTCCGTGGCAGGTGAGTATTCAGGCAAGAAGTGAACCTTTCTGTG
  GCGGCTCCATCCTCAACAAGTGGTGGATTCTCACTGCGGCTCACTGCTTATATTCCGAGGAGCTGTTTCC
  AGAAGAACTGAGTGTCGTGCTGGGGACCAACGACTTAACTAGCCCATCCATGGAAATAAAGGAGGTCGCC
  AGCATCATTCTTCACAAAGACTTTAAGAGAGCCAACATGGACAATGACATTGCCTTGCTGCTGCTGGCTT
  CGCCCATCAAGCTCGATGACCTGAAGGTGCCCATCTGCCTCCCCACGCAGCCCGGCCCTGCCACATGGCG
  CGAATGCTGGGTGGCAGGTTGGGGCCAGACCAATGCTGCTGACAAAAACTCTGTGAAAACGGATCTGATG
  AAAGCGCCAATGGTCATCATGGACTGGGAGGAGTGTTCAAAGATGTTTCCAAAACTTACCAAAAATATGC
  TGTGTGCCGGATACAAGAATGAGAGCTATGATGCCTGCAAGCAAAGCTATTTTCCCACTTTACAAAGAAT
  GAACACCGGAAGCTCTCAAACAAAGCCGCCTGGGTCTCACACCTTTCATCTGCAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231117 representing NM_001197020
  Red=Cloning site Green=Tags(s)
  
  MLLFSVLLLLSLVTGTQLGPRTPLPEAGVAILGRARGAHRPQPPHPPSPVSECGDRSIFEGRTRYSRITG
  GMEAEVGEFPWQVSIQARSEPFCGGSILNKWWILTAAHCLYSEELFPEELSVVLGTNDLTSPSMEIKEVA
  SIILHKDFKRANMDNDIALLLLASPIKLDDLKVPICLPTQPGPATWRECWVAGWGQTNAADKNSVKTDLM
  KAPMVIMDWEECSKMFPKLTKNMLCAGYKNESYDACKQSYFPTLQRMNTGSSQTKPPGSHTFHLQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001197020
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197020.1, NP_001183949.1
• RefSeq ORF: 831
• Locus ID: 203074
• Protein Families: Druggable Genome
• MW: 31 kDa
• Gene Summary: This gene encodes a member of a group of membrane-anchored chymotrypsin (S1)-like serine proteases. The enocoded protein is primarily expressed in the Leydig and Sertoli cells of the testis and may be involved in male fertility. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]