ARHGAP8 (NM_001198726) Human Tagged ORF Clone

CAT#: RC231149

ARHGAP8 (Myc-DDK-tagged)-Human Rho GTPase activating protein 8 (ARHGAP8), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP8
• Synonyms: BPGAP1; PP610
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231149 representing NM_001198726
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACATGGCATTCTGC
  AGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCACCCTCCCA
  CGAGCTGGACCACCAGCGGCTGCTGGAGTATTTGAAGTACACACTGGACCAATACGTTGAGAACGATTAT
  ACCATCGTCTATTTCCACTACGGGCTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCAT
  ACAAGGAGTTCGATAGGAAGTACAAGAAGAACTTGAAGGCCCTCTACGTGGTGCACCCCACCAGCTTCAT
  CAAGGTCCTGTGGAACATCTTGAAGCCCCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAAC
  TACCTGAGTGAGCTCCACGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACG
  ATGAGAAGCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCCCCC
  GCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGGCGAACTCATCCCC
  CCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCGCACCGAGGGCCTGTTCCGGAGAT
  CCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCTCTACAACCAAGGGAAGCCCGTGAACTTTGACGA
  CTACGGGGACATTCACATCCCTGCCGTGATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTG
  ACCTTCCAGGCCTACGAGCAGATTCTCGGGATCACCTGTGTCCCGGGAGAGCATCTTCAACAAAATGAAC
  AGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231149 representing NM_001198726
  Red=Cloning site Green=Tags(s)
  
  MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDY
  TIVYFHYGLNSRNKPSLGWLQSAYKEFDRKYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFN
  YLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIP
  PVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLL
  TFQAYEQILGITCVPGEHLQQNEQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198726
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198726.1, NP_001185655.1
• RefSeq ORF: 918
• Locus ID: 23779
• MW: 36.1 kDa
• Gene Summary: This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]