LIMS1 (NM_001193483) Human Tagged ORF Clone
CAT#: RC231171
- TrueORF®
LIMS1 (Myc-DDK-tagged)-Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 5
"NM_001193483" in other vectors (3)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LIMS1 |
Synonyms | PINCH; PINCH-1; PINCH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC231171 representing NM_001193483
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCGTGGCGGCCGGGATGACCCACAGCAACATGGCCAACGCCCTGGCCAGCGCCACTTGCGAGC GCTGCAAGGGCGGCTTTGCGCCCGCTGAGAAGATCGTGAACAGTAATGGGGAGCTGTACCATGAGCAGTG TTTCGTGTGCGCTCAGTGCTTCCAGCAGTTCCCAGAAGGACTCTTCTATGAGTTTGAAGGAAGAAAGTAC TGTGAACATGACTTTCAGATGCTCTTTGCCCCTTGCTGTCATCAGTGTGGTGAATTCATCATTGGCCGAG TTATCAAAGCCATGAATAACAGCTGGCATCCGGAGTGCTTCCGCTGTGACCTCTGCCAGGAAGTTCTGGC AGATATCGGGTTTGTCAAGAATGCTGGGAGACACCTGTGTCGCCCCTGTCATAATCGTGAGAAAGCCAGA GGCCTTGGGAAATACATCTGCCAGAAATGCCATGCTATCATCGATGAGCAGCCTCTGATATTCAAGAACG ACCCCTACCATCCAGACCATTTCAACTGCGCCAACTGCGGGAAGGAGCTGACTGCCGATGCACGGGAGCT GAAAGGGGAGCTATACTGCCTCCCATGCCATGATAAAATGGGGGTCCCCATCTGTGGTGCTTGCCGACGG CCCATCGAAGGGCGCGTGGTGAACGCTATGGGCAAGCAGTGGCATGTGGAGCATTTTGTTTGTGCCAAGT GTGAGAAACCCTTTCTTGGACATCGCCATTATGAGAGGAAAGGCCTGGCATATTGTGAAACTCACTATAA CCAGCTATTTGGTGATGTTTGCTTCCACTGCAATCGTGTTATAGAAGGTGATGTGGTCTCTGCTCTTAAT AAGGCCTGGTGCGTGAACTGCTTTGCCTGTTCTACCTGCAACACTAAATTAACACTCAAGAATAAGTTTG TGGAGTTTGACATGAAGCCAGTCTGTAAGAAGTGCTATGAGAAATTTCCATTGGAGCTGAAGAAAAGACT TAAGAAACTAGCTGAGACCTTAGGAAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231171 representing NM_001193483
Red=Cloning site Green=Tags(s) MLGVAAGMTHSNMANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKY CEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKAR GLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRR PIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALN KAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001193483 |
ORF Size | 1011 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001193483.1, NM_001193483.2, NP_001180412.1 |
RefSeq ORF | 1014 bp |
Locus ID | 3987 |
Cytogenetics | 2q12.3 |
Protein Families | Druggable Genome |
MW | 38.9 kDa |
Gene Summary | 'The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG231171 | LIMS1 (GFP-tagged) - Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 5 |
USD 460.00 |
|
RC231171L3 | Lenti-ORF clone of LIMS1 (Myc-DDK-tagged)-Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 5 |
USD 620.00 |
|
RC231171L4 | Lenti-ORF clone of LIMS1 (mGFP-tagged)-Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 5 |
USD 620.00 |
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