ALF (GTF2A1L) (NM_001193487) Human Tagged ORF Clone

CAT#: RC231236

GTF2A1L (Myc-DDK-tagged)-Human general transcription factor IIA, 1-like (GTF2A1L), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2A1L
• Synonyms: ALF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231236 representing NM_001193487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCCTCAACCCGGTGCTCTGGGAAACCAAGGTTTTGCAGTCTAAAGCAACAGAAGACTTCTTCA
  GAAATAGCATCCAATCACCTCTGTTTACTCTTCAGTTGCCGCACAGCTTGCACCAAACATTGCAATCGTC
  AACAGCATCATTAGTTATTCCTGCTGGTAGAACTCTTCCAAGTTTTACCACAGCAGAACTGGGCACTTCA
  AACTCCAGTGCAAACTTTACTTTTCCTGGTTATCCCATTCATGTACCAGCAGGTGTGACACTACAGACTG
  TATCTGGTCACCTTTATAAAGTCAATGTACCAATTATGGTGACAGAGACTTCTGGAAGAGCAGGTATTCT
  TCAGCATCCAATTCAGCAAGTATTTCAACAGCTTGGCCAGCCTTCAGTAATACAAACTAGTGTTCCACAA
  TTGAATCCATGGTCTCTTCAAGCAACTACTGAAAAATCACAGAGAATTGAAACCGTGCTACAGCAACCCG
  CAATTCTACCTTCTGGGCCAGTAGATAGGAAACACTTAGAAAATGCCACCAGTGATATACTTGTATCTCC
  TGGAAATGAGCATAAAATCGTGCCTGAAGCTTTGTTGTGTCATCAGGAAAGTTCTCACTATATCAGTCTT
  CCAGGTGTTGTATTTTCTCCACAGGTCTCTCAAACAAATTCTAATGTGGAGTCAGTGCTCAGTGGTTCAG
  CTAGCATGGCTCAAAATCTGCATGATGAGTCCCTCTCCACAAGCCCTCATGGGGCTCTCCACCAGCACGT
  GACTGATATTCAGCTTCATATTCTTAAAAATAGGATGTATGGATGTGATTCTGTAAAGCAACCAAGAAAT
  ATAGAGGAACCCAGCAACATACCTGTATCAGAGAAGGATTCTAATTCTCAGGTGGATTTAAGCATTCGGG
  TTACTGATGATGATATTGGTGAAATAATTCAAGTAGATGGAAGCGGTGATACATCTTCCAATGAAGAAAT
  AGGAAGTACAAGAGATGCAGATGAGAATGAATTTCTAGGGAATATTGACGGGGGAGATCTGAAGGTACCT
  GAAGAAGAAGCTGACAGTATTTCAAATGAGGATTCAGCCACAAACAGTAGTGATAATGAAGACCCTCAAG
  TAAACATTGTAGAAGAGGACCCTTTAAATTCTGGAGATGATGTTAGTGAACAGGATGTGCCAGACCTGTT
  TGACACGGATAATGTTATTGTCTGTCAGTATGATAAGATTCATCGAAGCAAGAACAAATGGAAATTCTAT
  TTGAAAGATGGTGTTATGTGTTTTGGAGGGAGAGACTATGTATTTGCAAAAGCCATTGGTGATGCAGAGT
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231236 representing NM_001193487
  Red=Cloning site Green=Tags(s)
  
  MACLNPVLWETKVLQSKATEDFFRNSIQSPLFTLQLPHSLHQTLQSSTASLVIPAGRTLPSFTTAELGTS
  NSSANFTFPGYPIHVPAGVTLQTVSGHLYKVNVPIMVTETSGRAGILQHPIQQVFQQLGQPSVIQTSVPQ
  LNPWSLQATTEKSQRIETVLQQPAILPSGPVDRKHLENATSDILVSPGNEHKIVPEALLCHQESSHYISL
  PGVVFSPQVSQTNSNVESVLSGSASMAQNLHDESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRN
  IEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEIIQVDGSGDTSSNEEIGSTRDADENEFLGNIDGGDLKVP
  EEEADSISNEDSATNSSDNEDPQVNIVEEDPLNSGDDVSEQDVPDLFDTDNVIVCQYDKIHRSKNKWKFY
  LKDGVMCFGGRDYVFAKAIGDAEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193487
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001193487.1, NM_001193487.2, NP_001180416.1
• RefSeq ORF: 1335
• Locus ID: 11036
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 49 kDa
• Gene Summary: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]